[NMR paper] Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.

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Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.

Related Articles Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.

Angew Chem Int Ed Engl. 2015 Dec 21;

Authors: Wang Y, Roose BW, Philbin JP, Doman JL, Dmochowski IJ

Abstract
A supramolecular strategy for detecting specific proteins in complex media by using hyperpolarized (129) Xe NMR is reported. A cucurbit[6]uril (CB[6])-based molecular relay was programmed for three sequential equilibrium conditions by designing a two-faced guest (TFG) that initially binds CB[6] and blocks the CB[6]-Xe interaction. The protein analyte recruits the TFG and frees CB[6] for Xe binding. TFGs containing CB[6]- and carbonic anhydrase II (CAII)-binding domains were synthesized in one or two steps. X-ray crystallography confirmed TFG binding to Zn(2+) in the deep CAII active-site cleft, which precludes simultaneous CB[6] binding. The molecular relay was reprogrammed to detect avidin by using a different TFG. Finally, Xe binding by CB[6] was detected in buffer and in E. coli cultures expressing CAII through ultrasensitive (129) Xe NMR spectroscopy.


PMID: 26692420 [PubMed - as supplied by publisher]



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