Knowledge of how effectors interact with RAS GTPases is key to understanding how these switch-like proteins function in cells. Effectors bind specifically to GTP-loaded RAS using RAS association (RA) or RAS binding domains (RBDs) that show wide-ranging affinities and thermodynamic characteristics. Both normal development and RAS-induced tumorigenesis depend on multiple distinct effector proteins that are frequently co-expressed and co-localized, suggesting an antagonistic nature to signaling...
[NMR paper] Molecular motions and interactions in aqueous solutions of thymosin-?4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Molecular motions and interactions in aqueous solutions of thymosin-?4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Related Articles Molecular motions and interactions in aqueous solutions of thymosin-?4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Chemphyschem. 2020 May 29;:
Authors: Bokor M, Tantos Á, Mészáros A, Jenei B, Haminda R, Tompa P, Tompa K
Abstract
Wide-line 1 H NMR measurements were extended and all results were interpreted in a thermodynamics* based new approach on...
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[NMR paper] Molecular motions and interactions in aqueous solutions of thymosin-ß4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Molecular motions and interactions in aqueous solutions of thymosin-ß4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Related Articles Molecular motions and interactions in aqueous solutions of thymosin-ß4, stabilin CTD and their 1:1 complex, studied by 1H-NMR spectroscopy.
Chemphyschem. 2017 Dec 23;:
Authors: Bokor M, Tantos Á, Mészáros A, Jenei B, Haminda R, Tompa P, Tompa K
Abstract
Wide-line 1H NMR measurements were extended and all results were reinterpreted in a thermodynamics based new approach on...
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[NMR paper] Monitoring the Interactions of a Ternary Complex Using NMR Spectroscopy: The Case of Sugars, Polyphenols, and Proteins.
Monitoring the Interactions of a Ternary Complex Using NMR Spectroscopy: The Case of Sugars, Polyphenols, and Proteins.
Monitoring the Interactions of a Ternary Complex Using NMR Spectroscopy: The Case of Sugars, Polyphenols, and Proteins.
Anal Chem. 2016 Dec 20;88(24):12470-12478
Authors: Faurie B, Dufourc EJ, Laguerre M, Pianet I
Abstract
Gaining insight into intermolecular interactions between multiple species is possible at an atomic level by looking at different parameters using different NMR techniques. In the specific...
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Role of Hydrophobic Interactions in the EncounterComplex Formation of the Plastocyanin and Cytochrome f Complex Revealed by Paramagnetic NMR Spectroscopy
Role of Hydrophobic Interactions in the EncounterComplex Formation of the Plastocyanin and Cytochrome f Complex Revealed by Paramagnetic NMR Spectroscopy
Sandra Scanu, Johannes M. Foerster, G. Matthias Ullmann and Marcellus Ubbink
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja4015452/aop/images/medium/ja-2013-015452_0009.gif
Journal of the American Chemical Society
DOI: 10.1021/ja4015452
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/I9ARcKlvRs8
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[NMR paper] Role of hydrophobic interactions in the encounter complex formation of plastocyanin and cytochrome f complex revealed by paramagnetic NMR spectroscopy.
Role of hydrophobic interactions in the encounter complex formation of plastocyanin and cytochrome f complex revealed by paramagnetic NMR spectroscopy.
Role of hydrophobic interactions in the encounter complex formation of plastocyanin and cytochrome f complex revealed by paramagnetic NMR spectroscopy.
J Am Chem Soc. 2013 Apr 29;
Authors: Scanu S, Förster J, Ullmann GM, Ubbink M
Abstract
Protein complex formation is thought to be at least a two-step process, in which the active complex is preceded by the formation of an encounter complex....
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[NMR paper] Analysis of SNARE complex/Synaptotagmin-1 Interactions by One-dimensional NMR Spectroscopy.
Analysis of SNARE complex/Synaptotagmin-1 Interactions by One-dimensional NMR Spectroscopy.
Related Articles Analysis of SNARE complex/Synaptotagmin-1 Interactions by One-dimensional NMR Spectroscopy.
Biochemistry. 2013 Apr 25;
Authors: Zhou A, Brewer KD, Rizo J
Abstract
Neurotransmitter release depends critically on the Ca2+ sensor synaptotagmin-1 and the SNARE proteins syntaxin-1, synaptobrevin and SNAP-25, which mediate membrane fusion by forming tight SNARE complexes that bridge the synaptic vesicle and plasma membranes. Interactions...
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[NMR paper] Solid-state 31P NMR spectroscopy of microcrystals of the Ras protein and its effector
Solid-state 31P NMR spectroscopy of microcrystals of the Ras protein and its effector loop mutants: comparison between crystalline and solution state.
Related Articles Solid-state 31P NMR spectroscopy of microcrystals of the Ras protein and its effector loop mutants: comparison between crystalline and solution state.
J Mol Biol. 2004 Sep 17;342(3):1033-40
Authors: Iuga A, Spoerner M, Kalbitzer HR, Brunner E
Cycling between a GTP bound "on" state and a GDP bound "off" state, guanine nucleotide-binding (GNB) proteins act as molecular switches....
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[NMR paper] High-pressure NMR study of the complex of a GTPase Rap1A with its effector RalGDS. A
High-pressure NMR study of the complex of a GTPase Rap1A with its effector RalGDS. A conformational switch in RalGDS revealed from non-linear pressure shifts.
Related Articles High-pressure NMR study of the complex of a GTPase Rap1A with its effector RalGDS. A conformational switch in RalGDS revealed from non-linear pressure shifts.
FEBS Lett. 2001 Oct 12;506(3):180-4
Authors: Inoue K, Maurer T, Yamada H, Herrmann C, Horn G, Kalbitzer HR, Akasaka K
Unusually large non-linear 1H and 15N nuclear magnetic resonance chemical shifts against...