The majority of base pairs in double-stranded DNA exist in the canonical Watson-Crick geometry. However, they can also adopt alternate Hoogsteen conformations in various complexes of DNA with proteins and small molecules, which are key for biological function and mechanism. While detection of Hoogsteen base pairs in large DNA complexes and assemblies poses considerable challenges for traditional structural biology techniques, we show here that multidimensional dynamic nuclear...
[NMR paper] Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Related Articles Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.
Biochem Soc Trans. 2020 May 07;:
Authors: Chakraborty A, Deligey F, Quach J, Mentink-Vigier F, Wang P, Wang T
Abstract
Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing...
Probing the surface of ?-Al2O3 by oxygen-17 dynamic nuclear polarization enhanced solid-state NMR spectroscopy #DNPNMR
From The DNP-NMR Blog:
Probing the surface of ?-Al2O3 by oxygen-17 dynamic nuclear polarization enhanced solid-state NMR spectroscopy #DNPNMR
Li, Wenzheng, Qiang Wang, Jun Xu, Fabien Aussenac, Guodong Qi, Xingling Zhao, Pan Gao, Chao Wang, and Feng Deng. “Probing the Surface of ?-Al2O3 by Oxygen-17 Dynamic Nuclear Polarization Enhanced Solid-State NMR Spectroscopy.” Physical Chemistry Chemical Physics 20, no. 25 (June 27, 2018): 17218–25.
https://doi.org/10.1039/C8CP03132K.
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11-25-2018 06:02 AM
Atomic structures of excited state Aâ??T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations
Atomic structures of excited state Aâ??T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations
Abstract
NMR relaxation dispersion studies indicate that in canonical duplex DNA, Watsonâ??Crick base pairs (bps) exist in dynamic equilibrium with short-lived low abundance excited state Hoogsteen bps. N1-methylated adenine (m1A) and guanine (m1G) are naturally occurring forms of damage that stabilize Hoogsteen bps in duplex DNA. NMR dynamic ensembles of DNA duplexes with m1Aâ??T Hoogsteen...
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04-19-2018 01:52 PM
DirectNMR Evidence that Transient Tautomeric andAnionic States in dG·dT Form Watson–Crick-like Base Pairs
DirectNMR Evidence that Transient Tautomeric andAnionic States in dG·dT Form Watson–Crick-like Base Pairs
Eric S. Szymanski, Isaac J. Kimsey and Hashim M. Al-Hashimi
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b01156/20170320/images/medium/ja-2017-01156m_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.7b01156
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/sMXmx3Kniac
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03-21-2017 08:29 AM
Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy
From The DNP-NMR Blog:
Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy
Smith, A.N. and J.R. Long, Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy. Analytical Chemistry, 2016. 88(1): p. 122-132.
http://dx.doi.org/10.1021/acs.analchem.5b04376
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02-01-2016 05:12 PM
[NMR paper] NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by
NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Related Articles NMR scalar couplings across Watson-Crick base pair hydrogen bonds in DNA observed by transverse relaxation-optimized spectroscopy.
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14147-51
Authors: Pervushin K, Ono A, Fernández C, Szyperski T, Kainosho M, Wüthrich K
This paper describes the NMR observation of 15N---15N and 1H---15N scalar couplings across the hydrogen bonds in Watson-Crick base pairs...
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11-17-2010 11:15 PM
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocry
Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Dynamic nuclear polarization-enhanced solid-state NMR spectroscopy of GNNQQNY nanocrystals and amyloid fibrils.
Phys Chem Chem Phys. 2010 Jun 14;12(22):5911-9
Authors: Debelouchina GT, Bayro MJ, van der Wel PC, Caporini MA, Barnes AB, Rosay M, Maas WE, Griffin RG
Dynamic nuclear polarization (DNP) utilizes the...
Probing Watson-Crick and Hoogsteen base pairing in duplex DNA using dynamic nuclear polarization solid-state NMR spectroscopy
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