Related ArticlesProbing Protein Quinary Interactions by in-cell NMR.
Biochemistry. 2015 Apr 20;
Authors: Majumder S, Xue J, DeMott CM, Reverdatto S, Burz DS, Shekhtman A
Abstract
Historically introduced by McConkey to explain the slow mutation rate of highly abundant proteins, protein weak (quinary) interactions are an emergent property of living cells. The protein complexes that result from quinary interactions are transient and thus difficult to study biochemically in vitro. Cross-correlated relaxation induced polarization transfer (CRIPT) based in-cell NMR allows the characterization of protein quinary interactions with atomic resolution inside live prokaryotic and eu-karyotic cells. We show that RNAs are an important component of protein quinary interactions. Protein quinary interactionss are unique to the target protein and affect physicochemical properties, protein ac-tivity, and interactions with drugs.
PMID: 25894651 [PubMed - as supplied by publisher]
[NMR paper] Using Singular Value Decomposition to Characterize Protein-Protein Interactions by In-cell NMR Spectroscopy.
Using Singular Value Decomposition to Characterize Protein-Protein Interactions by In-cell NMR Spectroscopy.
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Chembiochem. 2014 Apr 1;
Authors: Majumder S, Demott CM, Burz DS, Shekhtman A
Abstract
Distinct differences between how model proteins interact in-cell and in vitro suggest that the cytosol might have a profound effect in modulating protein-protein and/or protein-ligand interactions that are...
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[NMR paper] Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions.
Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions.
Related Articles Cell-free protein synthesis in an autoinduction system for NMR studies of protein-protein interactions.
J Biomol NMR. 2005 Jul;32(3):235-41
Authors: Ozawa K, Jergic S, Crowther JA, Thompson PR, Wijffels G, Otting G, Dixon NA
Cell-free protein synthesis systems provide facile access to proteins in a nascent state that enables formation of soluble, native protein-protein complexes even if one of the protein components is prone...
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[NMR paper] Probing the binding entropy of ligand-protein interactions by NMR.
Probing the binding entropy of ligand-protein interactions by NMR.
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Chembiochem. 2005 Sep;6(9):1585-91
Authors: Homans SW
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[NMR paper] NMR probing of protein-protein interactions using reporter ligands and affinity tags.
NMR probing of protein-protein interactions using reporter ligands and affinity tags.
Related Articles NMR probing of protein-protein interactions using reporter ligands and affinity tags.
J Am Chem Soc. 2004 Feb 18;126(6):1636-7
Authors: Ludwiczek ML, Baminger B, Konrat R
A novel method is proposed for the detection and quantification of protein-protein interactions in solution. In this approach, one protein binding partner is tagged with a ligand binding domain, and protein-protein interaction is monitored via changes in the NMR relaxation...
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[NMR paper] Probing the kinetic landscape of transient peptide-protein interactions by use of pep
Probing the kinetic landscape of transient peptide-protein interactions by use of peptide (15)n NMR relaxation dispersion spectroscopy: binding of an antithrombin peptide to human prothrombin.
Related Articles Probing the kinetic landscape of transient peptide-protein interactions by use of peptide (15)n NMR relaxation dispersion spectroscopy: binding of an antithrombin peptide to human prothrombin.
J Am Chem Soc. 2003 Oct 15;125(41):12432-42
Authors: Tolkatchev D, Xu P, Ni F
Protein-ligand interactions may lead to the formation of multiple...
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11-24-2010 09:16 PM
[NMR paper] Probing residual interactions in unfolded protein states using NMR spin relaxation te
Probing residual interactions in unfolded protein states using NMR spin relaxation techniques: an application to delta131delta.
Related Articles Probing residual interactions in unfolded protein states using NMR spin relaxation techniques: an application to delta131delta.
J Am Chem Soc. 2003 Oct 1;125(39):11988-92
Authors: Choy WY, Kay LE
Residual interactions in delta131delta, a large disordered fragment of staphylococcal nuclease, have been probed at two different pHs using backbone (15)N and side-chain methyl (2)H NMR spin relaxation...
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[NMR paper] Probing site-specific interactions in protein-DNA complexes using heteronuclear NMR s
Probing site-specific interactions in protein-DNA complexes using heteronuclear NMR spectroscopy and molecular modeling: binding of Cro repressor to OR3.
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J Biomol Struct Dyn. 1998 Aug;16(1):13-20
Authors: Edwards CA, Tung CS, Silks LA, Gatewood JM, Fee JA, Mariappan SV
In this paper, a general method is developed to study site-specific interactions in DNA-protein complexes...
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[NMR paper] Probing carbohydrate-protein interactions by high-resolution NMR spectroscopy.
Probing carbohydrate-protein interactions by high-resolution NMR spectroscopy.
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Adv Exp Med Biol. 1998;435:29-38
Authors: Homans SW, Field RA, Milton MJ, Probert M, Richardson JM
Probing Protein Quinary Interactions by in-cell NMR.
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