Related ArticlesProbing Medin Monomer Structure and its Amyloid Nucleation Using (13)C-Direct Detection NMR in Combination with Structural Bioinformatics.
Sci Rep. 2017 Mar 22;7:45224
Authors: Davies HA, Rigden DJ, Phelan MM, Madine J
Abstract
Aortic medial amyloid is the most prevalent amyloid found to date, but remarkably little is known about it. It is characterised by aberrant deposition of a 5.4 kDa protein called medin within the medial layer of large arteries. Here we employ a combined approach of ab initio protein modelling and (13)C-direct detection NMR to generate a model for soluble monomeric medin comprising a stable core of three ?-strands and shorter more labile strands at the termini. Molecular dynamics simulations suggested that detachment of the short, C-terminal ?-strand from the soluble fold exposes key amyloidogenic regions as a potential site of nucleation enabling dimerisation and subsequent fibril formation. This mechanism resembles models proposed for several other amyloidogenic proteins suggesting that despite variations in sequence and protomer structure these proteins may share a common pathway for amyloid nucleation and subsequent protofibril and fibril formation.
[NMR paper] Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Eur Biophys J. 2013 Dec;42(11-12):803-10
Authors: Usachev KS, Filippov AV,...
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07-06-2014 08:28 PM
Speeding up direct 15N detection: hCaN 2D NMR experiment
Speeding up direct 15N detection: hCaN 2D NMR experiment
Abstract Experiments detecting low gyromagnetic nuclei have recently been proposed to utilize the relatively slow relaxation properties of these nuclei in comparison to 1H. Here we present a new type of 15N direct-detection experiment. Like the previously proposed CaN experiment (Takeuchi et al. in J Biomol NMR 47:271â??282, 2010), the hCaN experiment described here sequentially connects amide 15N resonances, but utilizes the initial high polarization and the faster recovery of the 1H nucleus to shorten the recycling delay. This...
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11-14-2011 08:45 AM
Probing water-accessibility in HET-s(218-289) amyloid fibrils by solid-state NMR.
Probing water-accessibility in HET-s(218-289) amyloid fibrils by solid-state NMR.
Probing water-accessibility in HET-s(218-289) amyloid fibrils by solid-state NMR.
J Mol Biol. 2010 Nov 18;
Authors: Van Melckebeke H, Schanda P, Gath J, Wasmer C, Verel R, Lange A, Meier BH, Böckmann A
Despite its importance in the context of conformational diseases, structural information is still sparse for protein fibrils. Hydrogen/deuterium exchange measurements of backbone amides allow to identify hydrogen-bonding patterns and reveal pertinent information about...
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11-26-2010 05:32 PM
[NMR paper] Structural-functional bioinformatics: knowledge-based NMR interpretation.
Structural-functional bioinformatics: knowledge-based NMR interpretation.
Related Articles Structural-functional bioinformatics: knowledge-based NMR interpretation.
Stud Health Technol Inform. 1998;52 Pt 1:365-6
Authors: Kulikowski CA, Zimmerman D, Montelione G, Anderson S
This paper describes a knowledge-based approach to a problem of structural-functional bioinformatics, specifically the determination of protein structure through the automated analysis of NMR data. Highly successful results in carrying out sequence-specific assignments of...
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11-17-2010 11:06 PM
Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy.
Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy.
Related Articles Direct observation of minimum-sized amyloid fibrils using solution NMR spectroscopy.
Protein Sci. 2010 Oct 8;
Authors: Yoshimura Y, Sakurai K, Lee YH, Ikegami T, Chatani E, Naiki H, Goto Y
It is challenging to investigate the structure and dynamics of amyloid fibrils at the residue and atomic resolution due to their high molecular weight and heterogeneous properties. Here, we employed solution nuclear magnetic resonance (NMR) spectroscopy to...
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10-12-2010 02:52 PM
[Question from NMRWiki Q&A forum] How to set up 15N direct detection on UnityInova?
How to set up 15N direct detection on UnityInova?
Has anyone recorded directly detected 15N spectra on UnityInova? We have a triple resonance HCN probe. The sample I've used was natural abundance 1M NaNO3 - sodium nitrate.
I've tried to set up cable analogously to 13C detection - through the second preamp, but could not see any signal at all.
Check if somebody has answered this question on NMRWiki QA forum
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08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] What has been done with direct detection of Deuterium?
What has been done with direct detection of Deuterium?
Hello, I am trying to investigate what has been done in NMR using direct detection of deuterium in liquids. - Could you suggest any citations?
What kind of sensitivity gain there is in using broadband probe vs. lock channel?
Has anything been done/can be done to detect scalar couplings with protons like in COSY or TOCSY? Are HD couplings other than geminal detectable?
Thanks.
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08-22-2010 02:30 AM
NMR in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste
Nuclear Magnetic Resonance in Structural Biology - Alessandro Pintar, Protein Structure and Bioinformatics, ICGEB, Trieste
Thanks to advancements both in the theory and in the instrumentation, Nuclear Magnetic Resonance (NMR) has widened its use from small organic molecules to oligosaccharides, peptides, proteins, and nucleic acids. Together with X-ray crystallography, NMR is the only technique that can provide structural information at the atomic level. However, applications of NMR are not limited to 3D structure calculation: it can be used to study flexible biomolecules (peptides,...
Probing Medin Monomer Structure and its Amyloid Nucleation Using (13)C-Direct Detection NMR in Combination with Structural Bioinformatics.
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