The lateral pressure profile constitutes an important physical property of lipid bilayers, influencing the binding, insertion, and function of membrane-active peptides, such as antimicrobial peptides. In this study, we demonstrate that the lateral pressure profile can be manipulated using the peptides residing in different regions of the bilayer. A ^(19)F-labeled analogue of the amphiphilic peptide PGLa was used to probe the lateral pressure at different depths in the membrane. To evaluate the...
[NMR paper] Clustering of tetrameric influenza M2 peptides in lipid bilayers investigated by (19)F solid-state NMR
Clustering of tetrameric influenza M2 peptides in lipid bilayers investigated by (19)F solid-state NMR
The influenza M2 protein forms a drug-targeted tetrameric proton channel to mediate virus uncoating and carries out membrane scission to mediate virus release. While the proton channel function of M2 has been extensively studied, the mechanism by which M2 conducts membrane scission is still not well understood. Previous fluorescence and electron microscopy studies indicated that M2 tetramers concentrate at the neck of the budding virus in the host plasma membrane. However, molecular...
nmrlearner
Journal club
0
03-21-2022 04:01 AM
[NMR paper] Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Related Articles Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR.
Biomol NMR Assign. 2020 Jun 30;:
Authors: Schubeis T, Schwarzer TS, Le Marchand T, Stanek J, Movellan KT, Castiglione K, Pintacuda G, Andreas LB
Abstract
Most commonly small outer membrane proteins, possessing between 8 and 12 ?-strands, are not involved in transport but fulfill diverse functions...
nmrlearner
Journal club
0
07-02-2020 11:18 AM
[NMR paper] Assessing Interactions Between a Polytopic Membrane Protein and Lipid Bilayers Using Differential Scanning Calorimetry and Solid-State NMR.
Assessing Interactions Between a Polytopic Membrane Protein and Lipid Bilayers Using Differential Scanning Calorimetry and Solid-State NMR.
Related Articles Assessing Interactions Between a Polytopic Membrane Protein and Lipid Bilayers Using Differential Scanning Calorimetry and Solid-State NMR.
J Phys Chem B. 2018 Feb 19;:
Authors: Banigan JR, Leninger M, Her AS, Traaseth NJ
Abstract
It is known that the lipid composition within a cellular membrane can influence membrane protein structure and function. In this Article, we...
nmrlearner
Journal club
0
02-21-2018 12:45 AM
[NMR paper] Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Related Articles Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Magn Reson Chem. 2017 Feb 23;:
Authors: Zhang R, Sahu ID, Comer RG, Maltsev S, Dabney-Smith C, Lorigan GA
Abstract
KCNE1 is known to modulate the voltage-gated potassium channel ? subunit KCNQ1 to generate slowly activating potassium currents. This potassium channel is essential...
nmrlearner
Journal club
0
02-27-2017 09:45 AM
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Progression of NMR studies of membrane-active peptides from lipid bilayers to live cells
Publication date: Available online 27 December 2014
Source:Journal of Magnetic Resonance</br>
Author(s): M.-A. Sani , F. Separovic</br>
Understanding the structure of membrane-active peptides faces many challenges associated with the development of appropriate model membrane systems as the peptide structure depends strongly on the lipid environment. This perspective provides a brief overview of the approach taken to study antimicrobial and amyloid peptides in phospholipid...
nmrlearner
Journal club
0
12-28-2014 10:29 PM
[NMR paper] Investigation of the mechanism of action of novel amphipathic peptides: Insights from solid-state NMR studies of oriented lipid bilayers.
Investigation of the mechanism of action of novel amphipathic peptides: Insights from solid-state NMR studies of oriented lipid bilayers.
Related Articles Investigation of the mechanism of action of novel amphipathic peptides: Insights from solid-state NMR studies of oriented lipid bilayers.
Biochim Biophys Acta. 2014 Feb 6;
Authors: Fillion M, Noël M, Lorin A, Voyer N, Auger M
Abstract
We have investigated in the present study the effect of both non-selective and selective cationic 14-mer peptides on the lipid orientation of DMPC bilayers...
nmrlearner
Journal club
0
02-11-2014 09:58 PM
[NMR paper] Paramagnetic doping of a 7TM membrane protein in lipid bilayers by Gd(3+)-complexes for solid-state NMR spectroscopy.
Paramagnetic doping of a 7TM membrane protein in lipid bilayers by Gd(3+)-complexes for solid-state NMR spectroscopy.
Related Articles Paramagnetic doping of a 7TM membrane protein in lipid bilayers by Gd(3+)-complexes for solid-state NMR spectroscopy.
J Biomol NMR. 2013 Dec 4;
Authors: Ullrich SJ, Hölper S, Glaubitz C
Abstract
A considerable limitation of NMR spectroscopy is its inherent low sensitivity. Approximately 90*% of the measuring time is used by the spin system to return to its Boltzmann equilibrium after excitation, which is...
Probing and Manipulating the Lateral Pressure Profile in Lipid Bilayers Using Membrane-Active Peptides-A Solid-State 19F NMR Study
Linear Mode
