Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 7 January 2011
S. Bastien, Morin
Protein NMR - A Practical Guide - Solution NMR Assignment - Practice
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Assignment - Practice (for Analysis 2.1)
This section describes how the assignment principles described under Assignment - Theory can be but put into practice using the CCCPNmr Analysis software. There are several ways in which triple resonance backbone assignment, in particular, can be approached...
markber
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11-07-2011 01:25 AM
Protein NMR - A Practical Guide - Solution NMR Assignment Theory
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Assignment - Theory
The most simple and straight forward method of backbone resonance assignment involves the use of 15N,13C labelled protein and the measurement of CBCANNH and CBCA(CO)NNH spectra.
Large Proteins
Large proteins give worse NMR spectra, because they tumble more slowly. For this reason the CBCANNH and CBCA(CO)NNH spectra of larger proteins (> 150...
markber
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11-06-2011 09:42 PM
Protein NMR - A Practical Guide - Solution NMR Spectra
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Spectrum Descriptions
This page contains a list of the solution NMR experiments most commonly used in protein NMR assignment and structure calculation. For each experiment there is an illustration showing which atoms are observed (pink) and through which atoms magnetisation flows (light blue). An example spectrum shows what it should look like and particular features and...
markber
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11-06-2011 08:45 PM
Protein NMR - A Practical Guide
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Most books on Protein NMR focus on theoretical aspects and pulse sequences with only little space devoted to resonance assignment and structure calculations. At the same time many software manuals provide detailed information on how to use the software, but assume prior knowledge of the concepts of assignment and structure calculation. This has produced a gap in this area which these webpages aim to bridge by describing the ...
markber
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11-06-2011 08:22 PM
[Nature network NMR forum] Practical guide to protein NMR assignment (1 reply)
Practical guide to protein NMR assignment (1 reply)
There is a new website guiding new spectroscopists through the basics of assigning their favorite protein – click here
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