[NMR paper] The Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
The Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
Related ArticlesThe Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
J Agric Food Chem. 2017 May 12;:
Authors: Zhao Y, Chen MX, Kongstad KT, Jager AK, Staerk D
Abstract
The Worldwide increasing incidence of type 2 diabetes has fuelled an intensified search for food and herbal remedies with preventive and/or therapeutic properties. Polygonum cuspidatum Siebold & Zucc. (Polygonaceae) is used as a functional food in Japan and South Korea, and it's also a well-known traditional antidiabetic herb used in China. In this study, dual high-resolution ?-glucosidase and protein-tyrosine phosphatase 1B (PTP1B) inhibition profiling was used for identification of individual antidiabetic constituents directly from the crude ethyl acetate extract and fractions of P. cuspidatum. Subsequent preparative-scale HPLC was used to isolate a series of ?-glucosidase inhibitors, which after HPLC-HRMS and NMR analysis were identified as procyanidin B2 3,3''-O-di-gallate (3) and (-)-epicatechin gallate (5) with IC50 values of 0.42 ± 0.02 and 0.48 ± 0.0004 ?M, respectively, as well as a series of stilbene analogues with IC50 value in the range from 6.05 ± 0.05 ?M to 116.10 ± 2.04 ?M. In addition, (trans)-emodin-physcion bianthrone (15b) and (cis)-emodin-physcion bianthrone (15c) were identified as potent PTP1B inhibitors with IC50 values of 2.77 ± 1.23 and 7.29 ± 2.32 ?M, respectively. These findings show that P. cuspidatum is a potential functional food for management of type 2 diabetes.
PMID: 28497962 [PubMed - as supplied by publisher]
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Antidiabetic effects of glucokinase regulatory protein small-molecule disruptors - Nature.com
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Antidiabetic effects of glucokinase regulatory protein small-molecule disruptors
Nature.com
In hepatocytes, GK regulates glucose uptake and glycogen synthesis, suppresses glucose production3, 5, and is subject to the endogenous inhibitor GK regulatory protein (GKRP)6, 7, 8. During fasting, GKRP binds, inactivates and ..... 1H-, 13C- and 19F ...
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The Potential of Polygonum cuspidatum Root as an Antidiabetic Food - Dual High-Resolution ?-Glucosidase and PTP1B Inhibition Profiling Combined with HPLC-HRMS and NMR for Identification of Antidiabetic Constituents.
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