[NMR paper] Positional specifity of acetylxylan esterases on natural polysaccharide: an NMR study.

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Positional specifity of acetylxylan esterases on natural polysaccharide: an NMR study.

Related Articles Positional specifity of acetylxylan esterases on natural polysaccharide: an NMR study.

Biochim Biophys Acta. 2013 Jun;1830(6):3365-72

Authors: Uhliariková I, Vršanská M, McCleary BV, Biely P

Abstract
BACKGROUND: Microbial degradation of acetylated plant hemicelluloses involves besides enzymes cleaving the glycosidic linkages also deacetylating enzymes. A detailed knowledge of the mode of action of these enzymes is important in view of the development of efficient bioconversion of plant materials that did not undergo alkaline pretreatment leading to hydrolysis of ester linkages.
METHODS: In this work deacetylation of hardwood acetylglucuronoxylan by acetylxylan esterases from Streptomyces lividans (carbohydrate esterase family 4) and Orpinomyces sp. (carbohydrate esterase family 6) was monitored by (1)H-NMR spectroscopy.
RESULTS: The (1)H-NMR resonances of all acetyl groups in the polysaccharide were fully assigned. The targets of both enzymes are 2- and 3-monoacetylated xylopyranosyl residues and, in the case of the Orpinomyces sp. enzyme, also the 2,3-di-O-acetylated xylopyranosyl residues. Both enzymes do not recognize as a substrate the 3-O-acetyl group on xylopyranosyl residues ?-1,2-substituted with 4-O-methyl-d-glucuronic acid.
CONCLUSIONS: The (1)H-NMR spectroscopy approach to study positional and substrate specificity of AcXEs outlined in this work appears to be a simple way to characterize catalytic properties of enzymes belonging to various CE families.
SIGNIFICANCE: The results contribute to development of efficient and environmentally friendly procedures for enzymatic degradation of plant biomass.


PMID: 23375723 [PubMed - indexed for MEDLINE]



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