Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction.
Structure. 2011 Jan 12;19(1):26-36
Authors: de Paula VS, Razzera G, Barreto-Bergter E, Almeida FC, Valente AP
Defensins are essentially ancient natural antibiotics with potent activity extending from lower organisms to humans. Sd5 is a recently described antifungal defensin that appears to be the result of a recent gain of function. We reported here the solution NMR*structure of Sd5 and characterized the backbone dynamics in the free state and in the presence of*membrane models. (15)N relaxation dispersion measurements indicate intrinsic conformational exchange processes, showing two clear distinct k(ex), 490 and 1800 s(-1). These multiple motions may be related to transient twisting or breathing of the ? helix and ? sheet. The stages of membrane recognition and disruption by Sd5 over a large timescale range were mapped and demonstrated that Sd5 in solution sampled an ensemble of different conformations, of which a subset is selected upon membrane binding. Defensins share similar structures, but we demonstrated here that their dynamics can be extremely diverse.
[NMR paper] Investigating the dynamic properties of the transmembrane segment of phospholamban in
Investigating the dynamic properties of the transmembrane segment of phospholamban incorporated into phospholipid bilayers utilizing 2H and 15N solid-state NMR spectroscopy.
Related Articles Investigating the dynamic properties of the transmembrane segment of phospholamban incorporated into phospholipid bilayers utilizing 2H and 15N solid-state NMR spectroscopy.
Biochemistry. 2004 Nov 9;43(44):13899-909
Authors: Tiburu EK, Karp ES, Dave PC, Damodaran K, Lorigan GA
(2)H and (15)N solid-state NMR spectroscopic techniques were used to investigate...
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[NMR paper] Multiple quantum filtered NMR studies of the interaction between collagen and water i
Multiple quantum filtered NMR studies of the interaction between collagen and water in the tendon.
Related Articles Multiple quantum filtered NMR studies of the interaction between collagen and water in the tendon.
J Am Chem Soc. 2002 Mar 27;124(12):3125-32
Authors: Eliav U, Navon G
We studied the physical processes and the chemical reactions involved in magnetization transfer between water and large proteins, such as collagen, in bovine Achilles tendon. Since the NMR spectrum for such proteins is broadened by very large dipolar interactions,...
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[NMR paper] Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensin
Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action.
Related Articles Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action.
J Mol Biol. 2002 Jan 25;315(4):749-57
Authors: Almeida MS, Cabral KM, Kurtenbach E, Almeida FC, Valente AP
Pisum sativum defensin 1 (Psd1) is a 46 amino acid residue plant defensin isolated from seeds of pea. The three-dimensional structure in...
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[NMR paper] Essential dynamics from NMR clusters: dynamic properties of the Myb DNA-binding domai
Essential dynamics from NMR clusters: dynamic properties of the Myb DNA-binding domain and a hinge-bending enhancing variant.
Related Articles Essential dynamics from NMR clusters: dynamic properties of the Myb DNA-binding domain and a hinge-bending enhancing variant.
Methods. 1998 Mar;14(3):318-28
Authors: van Aalten DM, Grotewold E, Joshua-Tor L
Application of the "essential dynamics" method to the NMR cluster of structures for the R2R3 DNA-binding domain of the mouse c-Myb transcriptional activator is described. Using this method, large...
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[NMR paper] The dynamic properties of the M121H azurin metal site as studied by NMR of the parama
The dynamic properties of the M121H azurin metal site as studied by NMR of the paramagnetic Cu(II) and Co(II) metalloderivatives.
Related Articles The dynamic properties of the M121H azurin metal site as studied by NMR of the paramagnetic Cu(II) and Co(II) metalloderivatives.
J Biol Chem. 1998 Jan 2;273(1):177-85
Authors: Salgado J, Kroes SJ, Berg A, Moratal JM, Canters GW
The M121H azurin mutant in solution presents various species in equilibrium that can be detected and studied by 1H NMR of the Cu(II) and Co(II) paramagnetic...
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[NMR paper] Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P N
Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P NMR.
Related Articles Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P NMR.
J Biochem. 1994 Feb;115(2):270-8
Authors: Odahara T, Nishimoto S, Katsutani N, Kyogoku Y, Morimoto Y, Matsushiro A, Akutsu H
The dynamic properties of nucleic acids in five different types of intact supramolecular systems, namely, chicken erythrocyte chromatin, the wild type and a deletion mutant of the lambda phage, lipid-containing phage PM2, and...
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[NMR paper] Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P N
Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P NMR.
Related Articles Dynamic properties of nucleic acids in biosupramolecular systems, as studied by 31P NMR.
J Biochem. 1994 Feb;115(2):270-8
Authors: Odahara T, Nishimoto S, Katsutani N, Kyogoku Y, Morimoto Y, Matsushiro A, Akutsu H
The dynamic properties of nucleic acids in five different types of intact supramolecular systems, namely, chicken erythrocyte chromatin, the wild type and a deletion mutant of the lambda phage, lipid-containing phage PM2, and...
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[NMR paper] Dynamic properties of proteins from NMR spectroscopy.
Dynamic properties of proteins from NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Dynamic properties of proteins from NMR spectroscopy.
Curr Opin Biotechnol. 1993 Aug;4(4):385-91
Authors: Palmer AG
Two-dimensional proton-detected heteronuclear nuclear magnetic resonance spectroscopy has been used to measure 13C and 15N spin-relaxation rate constants for several proteins. Generalized order parameters and effective internal correlation times have been...
Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction.
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