Related ArticlesPopulating the equilibrium molten globule state of apomyoglobin under conditions suitable for structural characterization by NMR.
FEBS Lett. 1997 Nov 3;417(1):92-6
Authors: Eliezer D, Jennings PA, Dyson HJ, Wright PE
Conditions have been determined under which the equilibrium molten globule state of apomyoglobin is stable and remains monomeric for periods of time sufficient for the application of three-dimensional heteronuclear NMR experiments. The quality of initial two-dimensional NMR spectra suggests that sequence-specific assignments can be made for a majority of the protein resonances under these conditions. A pH titration of the protein followed using two-dimensional 1H-15N correlation experiments indicates that the equilibrium intermediate undergoes fast exchange on the chemical shift time scale with the unfolded state and intermediate time scale exchange with the native state, and suggests a strategy to assist with backbone resonance assignments. The conditions and techniques described may be applicable to the characterization of other equilibrium folding intermediates.
[NMR paper] Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NM
Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NMR spin-diffusion study.
Related Articles Water-protein interactions in the molten-globule state of carbonic anhydrase b: an NMR spin-diffusion study.
Protein Sci. 2000 Aug;9(8):1540-7
Authors: Kutyshenko VP, Cortijo M
We have used the homonuclear Overhauser effect (NOE) to characterize a model protein: carbonic anhydrase B. We have obtained NOE difference spectra for this protein, centering the on-resonance signals either at the methyl-proton or at the...
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[NMR paper] Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumi
Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumin: a (19)F-NMR study.
Related Articles Side chain accessibility and dynamics in the molten globule state of alpha-lactalbumin: a (19)F-NMR study.
Biochemistry. 2000 Jan 18;39(2):372-80
Authors: Bai P, Luo L, Peng Z
The molten globule state of alpha-lactalbumin (alpha-LA) has been considered a prototype of partially folded proteins. Despite the importance of molten globules in understanding the mechanisms of protein folding and its relevance to some biological...
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[NMR paper] Structural characterization of the molten globule and native states of ovalbumin: a 1
Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study.
Related Articles Structural characterization of the molten globule and native states of ovalbumin: a 1H NMR study.
J Pept Res. 1997 Dec;50(6):465-74
Authors: Sogami M, Era S, Koseki T, Nagai N
Molecular characteristics of ovalbumin (OVA) in the acidic (pD 3.08, the E-form) and neutral regions were studied by measuring effective radii, 1H NMR spectra, spin-echo 1H NMR spectra and cross-relaxation times (TIS) from irradiated to observed protein...
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[NMR paper] A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Related Articles A residue-specific NMR view of the non-cooperative unfolding of a molten globule.
Nat Struct Biol. 1997 Aug;4(8):630-4
Authors: Schulman BA, Kim PS, Dobson CM, Redfield C
Molten globules are partially folded forms of proteins that are thought to be general intermediates in protein folding. Nonetheless, there is limited structural information about such species because they possess conformational heterogeneity and complex dynamical properties that...
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[NMR paper] The native state of apomyoglobin described by proton NMR spectroscopy: the A-B-G-H in
The native state of apomyoglobin described by proton NMR spectroscopy: the A-B-G-H interface of wild-type sperm whale apomyoglobin.
Related Articles The native state of apomyoglobin described by proton NMR spectroscopy: the A-B-G-H interface of wild-type sperm whale apomyoglobin.
Proteins. 1996 Jul;25(3):267-85
Authors: Lecomte JT, Kao YH, Cocco MJ
Proton nuclear magnetic resonance spectroscopy was applied to sperm whale apomyoglobin to describe the conformation adopted by the protein under native conditions. The study focused on the A-B-G-H...
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[NMR paper] Is apomyoglobin a molten globule? Structural characterization by NMR.
Is apomyoglobin a molten globule? Structural characterization by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Is apomyoglobin a molten globule? Structural characterization by NMR.
J Mol Biol. 1996 Nov 8;263(4):531-8
Authors: Eliezer D, Wright PE
Multi-dimensional heteronuclear NMR spectroscopy has been used to obtain structural information on isotopically labeled recombinant sperm whale apomyoglobin in the native state at pH 6.1. Assignments for backbone...
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[NMR paper] Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deut
Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Stability of alpha-helices in a molten globule state of cytochrome c by hydrogen-deuterium exchange and two-dimensional NMR spectroscopy.
J Mol Biol. 1995 Apr 7;247(4):682-8
Authors: Kuroda Y, Endo S, Nagayama K, Wada A
To distinguish between intrinsically stable helices and those...
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[NMR paper] Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumi
Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumin: a two-dimensional NMR study.
Related Articles Structure and dynamics of the acid-denatured molten globule state of alpha-lactalbumin: a two-dimensional NMR study.
Biochemistry. 1993 Feb 23;32(7):1707-18
Authors: Alexandrescu AT, Evans PA, Pitkeathly M, Baum J, Dobson CM
Two-dimensional 1H-NMR spectroscopy has been used to study the acid-denatured molten globule (A-state) of alpha-lactalbumin. The NMR spectra show that chemical shift dispersion is limited...