Polysorbate-80 (PS80) and histidine are common excipients in vaccine and therapeutic protein formulation. A simple quantitative NMR method to measure both PS80 and histidine in human papillomavirus (HPV) virus-like particle (VLP) vaccine for aqueous and alum-containing samples is described. The new NMR method is compared to current colorimetric methods for PS80 and RP HPLC for histidine. The new NMR method is comparable to current assays with an advantage of a simpler sample treatment for PS80....
[NMR paper] Protein dynamics in the solid-state from 2H NMR lineshape analysis. III. MOMD in the presence of Magic Angle Spinning.
Protein dynamics in the solid-state from 2H NMR lineshape analysis. III. MOMD in the presence of Magic Angle Spinning.
Related Articles Protein dynamics in the solid-state from 2H NMR lineshape analysis. III. MOMD in the presence of Magic Angle Spinning.
Solid State Nucl Magn Reson. 2017 Nov 21;:
Authors: Meirovitch E, Liang Z, Freed JH
Abstract
We report on a new approach to the analysis of dynamic NMR lineshapes from polycrystalline (i.e., macroscopically disordered) samples in the presence of Magic Angle Spinning (MAS). This...
nmrlearner
Journal club
0
12-07-2017 11:34 AM
[NMR paper] Protonation equilibria and pore-opening structure of the dual-histidine influenza B virus M2 transmembrane proton channel from solid-state NMR.
Protonation equilibria and pore-opening structure of the dual-histidine influenza B virus M2 transmembrane proton channel from solid-state NMR.
Related Articles Protonation equilibria and pore-opening structure of the dual-histidine influenza B virus M2 transmembrane proton channel from solid-state NMR.
J Biol Chem. 2017 Sep 11;:
Authors: Williams JK, Shcherbakov AA, Wang J, Hong M
Abstract
The influenza A and B viruses are the primary cause of seasonal flu epidemics. Common to both viruses is the M2 protein, a homo-tetrameric...
nmrlearner
Journal club
0
09-13-2017 08:48 PM
[NMR paper] Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
Effect of Polysorbate 20 and Polysorbate 80 on the Higher Order Structure of a Monoclonal Antibody and its Fab and Fc Fragments Probed Using 2D NMR.
J Pharm Sci. 2017 Aug 23;:
Authors: Singh SM, Bandi S, Jones DNM, Mallela KMG
Abstract
We examined how polysorbate 20 (PS20; Tween 20) and polysorbate 80 (PS80; Tween 80) affect the higher order structure of a monoclonal antibody (mAb)...
nmrlearner
Journal club
0
08-28-2017 10:59 AM
[NMR paper] Monitoring the Disassembly of Virus-like Particles by 19F-NMR.
Monitoring the Disassembly of Virus-like Particles by 19F-NMR.
Related Articles Monitoring the Disassembly of Virus-like Particles by 19F-NMR.
J Am Chem Soc. 2017 Mar 28;:
Authors: Leung RL, Robinson MD, Ajabali AA, Karunanithy G, Lyons B, Raj R, Raoufmoghaddam S, Mohammed S, Claridge TD, Baldwin AJ, Davis BG
Abstract
Virus-like particles (VLPs) are stable protein cages derived from virus coats. They have been used extensively as biomolecular platforms, e.g. nanocarriers or vaccines, but a convenient in situ technique is lacking...
nmrlearner
Journal club
0
03-30-2017 06:42 PM
[NMR paper] Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles.
Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Helical Conformation in the CA-SP1 Junction of the Immature HIV-1 Lattice Determined from Solid-State NMR of Virus-like Particles.
J Am Chem Soc. 2016 Sep 21;138(37):12029-12032
Authors: Bayro MJ, Ganser-Pornillos BK, Zadrozny KK, Yeager M, Tycko R
Abstract
Maturation of...
nmrlearner
Journal club
0
09-22-2016 06:31 AM
Application of virus-like particles (VLP) to NMR characterization of viral membrane protein interactions
Application of virus-like particles (VLP) to NMR characterization of viral membrane protein interactions
Abstract
The membrane proteins of viruses play critical roles in the virus life cycle and are attractive targets for therapeutic intervention. Virus-like particles (VLP) present the possibility to study the biochemical and biophysical properties of viral membrane proteins in their native environment. Specifically, the VLP constructs contain the entire protein sequence and are comprised of native membrane components including lipids, cholesterol,...
nmrlearner
Journal club
0
02-29-2016 04:37 PM
[NMR paper] Application of virus-like particles (VLP) to NMR characterization of viral membrane protein interactions.
Application of virus-like particles (VLP) to NMR characterization of viral membrane protein interactions.
Application of virus-like particles (VLP) to NMR characterization of viral membrane protein interactions.
J Biomol NMR. 2016 Feb 26;
Authors: Antanasijevic A, Kingsley C, Basu A, Bowlin TL, Rong L, Caffrey M
Abstract
The membrane proteins of viruses play critical roles in the virus life cycle and are attractive targets for therapeutic intervention. Virus-like particles (VLP) present the possibility to study the biochemical...
nmrlearner
Journal club
0
02-28-2016 03:10 PM
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products
Journal Highlight: Quantitative NMR: An applicable method for quantitative analysis of medicinal plant extracts and herbal products
http://www.spectroscopynow.com/common/images/thumbnails/13abcca009b.jpgA quantitative NMR method has been reported for quantitative analysis of three medicinal plant extracts and their herbal products without the need of authentic standards.
Source: Spectroscopynow.com