A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
J Invertebr Pathol. 2014 Oct 14;124C:51-56
Authors: Liu PF, Liu QH, Wu Y, Jie H
Abstract
White spot syndrome virus, which was a pathogen first found in 1992, had emerged globally affecting shrimp populations in aquaculture. Here, we comprehensively analyzed the metabolic changes of hepatopancreas from Litopenaeus vannamei which were infected with white spot syndrome virus by (1)H nuclear magnetic resonance (NMR). Through the NOESYPR1D spectrum combined with multi-variate pattern recognition analysis, including principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) models, significantly metabolic changes were observed in WSSV-infected groups compared with the control groups. In the first 48h, ?-glucose and ?-glucose were higher in the WSSV-infected group. Meanwhile, acetate, lactate, N-acetyl glycoprotein signals, lysine, tyrosine and lipid were significantly decreased in the WSSV-infected group. These results suggest that WSSV caused absorption inhibition of amino acids and disturbed protein metabolism as well as cell metabolism in favor of its replication. Our findings could also contribute to further understanding of disease mechanisms.
PMID: 25450952 [PubMed - as supplied by publisher]
[NMR paper] Malignancy-associated metabolic profiling of human glioma cell lines using 1H NMR spectroscopy.
Malignancy-associated metabolic profiling of human glioma cell lines using 1H NMR spectroscopy.
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Mol Cancer. 2014 Aug 27;13(1):197
Authors: Shao W, Gu J, Huang C, Liu D, Huang H, Huang Z, Lin Z, Yang W, Liu K, Lin D, Ji T
Abstract
BACKGROUND: Ambiguity in malignant transformation of glioma has made prognostic diagnosis very challenging. Tumor malignant transformation is closely correlated with specific alterations...
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08-29-2014 05:36 PM
Journal Highlight: 1H NMR based metabolic profiling in Crohn's disease by random forest methodology
Journal Highlight: 1H NMR based metabolic profiling in Crohn's disease by random forest methodology
http://www.spectroscopynow.com/common/images/thumbnails/146ed5b84e7.jpg1H NMR spectroscopy of the serum metabolites of patients with Crohn's disease, supported by random forest methodology, showed that valine and isoleucine are differentiating metabolites for diagnosis.
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06-30-2014 11:39 PM
[NMR paper] Structure of hen egg-white lysozyme solvated in TFE/water: a molecular dynamics simulation study based on NMR data.
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J Biomol NMR. 2013 Mar 14;
Authors: Eichenberger AP, van Gunsteren WF, Smith LJ
Abstract
Various experimental studies of hen egg white lysozyme (HEWL) in water and TFE/water clearly indicate structural differences between the native state and TFE state of HEWL, e.g. the helical content of the...
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03-16-2013 03:18 PM
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics.
Ecotoxicology. 2011 May 15;
Authors: Liu X, Yang C, Zhang L, Li L, Liu S, Yu J, You L, Zhou D, Xia C, Zhao J, Wu H
Cadmium is a non-essential element to living organisms and has become the severe contaminant in both seawater and sediment in the intertidal zones of the Bohai Sea. The halophyte, Suaeda salsa...
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05-17-2011 06:21 PM
Metabolic profiling of vitamin C deficiency in Guloâ??/â?? mice using proton NMR spectroscopy
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Abstract Nutrient deficiencies are an ongoing problem in many populations and ascorbic acid is a key vitamin whose mild or acute absence leads to a number of conditions including the famously debilitating scurvy. As such, the biochemical effects of ascorbate deficiency merit ongoing scrutiny, and the Gulo knockout mouse provides a useful model for the metabolomic examination of vitamin C deficiency. Like humans, these animals are incapable of synthesizing ascorbic acid but with dietary...
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03-03-2011 02:06 AM
Metabolic characterization of Palatinate German white wines according to sensory attributes, varieties, and vintages using NMR spectroscopy and multivariate data analyses
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Abstract 1H NMR (nuclear magnetic resonance spectroscopy) has been used for metabolomic analysis of â??Rieslingâ?? and â??Mueller-Thurgauâ?? white wines from the German Palatinate region. Diverse two-dimensional NMR techniques have been applied for the identification of metabolites, including phenolics. It is shown that sensory analysis correlates with NMR-based metabolic profiles of wine. 1H NMR data in combination...
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03-03-2011 02:06 AM
1H NMR-based metabolic profiling reveals inherent biological variation in yeast and nematode model systems
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Abstract The application of metabolomics to human and animal model systems is poised to provide great insight into our understanding of disease etiology and the metabolic changes that are associated with these conditions. However, metabolomic studies have also revealed that there is significant, inherent biological variation in human samples and even in samples from animal model systems where the animals are housed under carefully controlled conditions. This inherent biological...
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Magn Reson Imaging. 2003 Nov;21(9):1039-43
Authors: Ramani A, Aliev AE, Barker GJ, Tofts PS
We report a new approach for the identification of an independent method of studying the semi-solid pool of protons, i.e., protons with constrained motion as a...
A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on (1)H NMR spectroscopy.
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