Related ArticlesPhysiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
Langmuir. 2020 May 16;:
Authors: Meikle T, Keizer DW, Babon JJ, Drummond CJ, Separovic F, Conn CE, Yao S
Abstract
Lipidic inverse bicontinuous cubic phases (LCPs), formed via the spontaneous self-assembly of lipids such as monoolein, have found increasing applications in the stabilization and crystallization of integral membrane proteins for structural characterization using X-ray crystallography. Their use as effective drug release matrices has also been demonstrated. Nuclear magnetic resonance (NMR) spectroscopy, both solution and solid-state, has previously been employed for the characterization of LCPs and related systems. Herein, we report a number of novel features of solution NMR for probing the fundamental composition and structural properties of monoolein-based LCPs. These include: (1) more complete assignments of both 1H and 13C chemical shifts, (2) direct quantification of hydration level in LCPs using 1D 1H NMR, and (3) monitoring longer term stability of LCPs and evaluating alterations introduced into standard LCPs at the sub-molecular level.
PMID: 32418433 [PubMed - as supplied by publisher]
Cubic three-dimensional hybrid silica solids for nuclear hyperpolarization
From The DNP-NMR Blog:
Cubic three-dimensional hybrid silica solids for nuclear hyperpolarization
Baudouin, D., et al., Cubic three-dimensional hybrid silica solids for nuclear hyperpolarization. Chemical Science, 2016.
http://dx.doi.org/10.1039/C6SC02055K
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University of Lille: Postdoc: Characterization of the condensed and gaseous phases of flame retarted materials upon bu...
University of Lille: Postdoc: Characterization of the condensed and gaseous phases of flame retarted materials upon bu...
University of Lille:The objectives of this postdoc deals with the development of methods to characterize degraded materials by physico-chemical analytical methods (methods ex-situ). It will particularly focus on solid state NMR methodology and analytical methods (e.g. FTIRFrance
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09-03-2015 09:53 PM
University of Lille: Postdoc: Characterization of the condensed and gaseous phases of flame retarted materials upon burning
University of Lille: Postdoc: Characterization of the condensed and gaseous phases of flame retarted materials upon burning
University of Lille:The objectives of this postdoc deals with the development of methods to characterize degraded materials by physico-chemical analytical methods (methods ex-situ). It will particularly focus on solid state NMR methodology and analytical methods (e.g. FTIRFrance
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08-26-2015 11:52 PM
[NMR paper] Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
Distinguishing Bicontinuous Lipid Cubic Phases from Isotropic Membrane Morphologies Using (31)P Solid-State NMR Spectroscopy.
J Phys Chem B. 2015 Mar 27;
Authors: Yang Y, Yao H, Hong M
Abstract
Nonlamellar lipid membranes are frequently induced by proteins that fuse, bend, and cut membranes. Understanding the mechanism of action of these proteins requires the elucidation of the membrane morphologies...
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03-31-2015 07:17 PM
[NMR paper] Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach.
Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach.
Related Articles Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach.
J Biomol NMR. 2015 Jan 8;
Authors: Sinnige T, Houben K, Pritisanac I, Renault M, Boelens R, Baldus M
Abstract
The ?-barrel assembly machinery (BAM) is involved in folding and insertion of outer membrane proteins in Gram-negative bacteria, a process that is still poorly...
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01-09-2015 03:58 PM
Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach
Insight into the conformational stability of membrane-embedded BamA using a combined solution and solid-state NMR approach
Abstract
The β-barrel assembly machinery (BAM) is involved in folding and insertion of outer membrane proteins in Gram-negative bacteria, a process that is still poorly understood. With its 790 residues, BamA presents a challenge to current NMR methods. We utilized a â??divide and conquerâ?? approach in which we first obtained resonance assignments for BamAâ??s periplasmic POTRA domains 4 and 5 by solution NMR. Comparison of...
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[NMR paper] Solution and high-pressure NMR studies of the structure, dynamics and stability of the cross-reactive allergenic cod parvalbumin Gad m 1.
Solution and high-pressure NMR studies of the structure, dynamics and stability of the cross-reactive allergenic cod parvalbumin Gad m 1.
Related Articles Solution and high-pressure NMR studies of the structure, dynamics and stability of the cross-reactive allergenic cod parvalbumin Gad m 1.
Proteins. 2014 Aug 12;
Authors: Moraes AH, Ackerbauer D, Kostadinova M, Bublin M, de Oliveira GA, Ferreira F, Almeida FC, Breiteneder H, Valente AP
Abstract
Beta-parvalbumins from different fish species have been identified as the main...
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08-15-2014 12:53 PM
[NMR paper] Native like structure and stability of apo AI in a n-propanol/water solution as deter
Native like structure and stability of apo AI in a n-propanol/water solution as determined by 13C NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Native like structure and stability of apo AI in a n-propanol/water solution as determined by 13C NMR.
FEBS Lett. 1995 Mar 13;361(1):29-34
Authors: Leroy A, Lippens G, Wieruszeski JM, Parra HJ, Fruchart JC
To elucidate the molecular details of the conformation of apolipoprotein AI (apo AI), we have developed an approach...
Physiochemical Characterization and Stability of Lipidic Cubic Phases by Solution NMR.
Linear Mode
