Designing probes for real-time imaging of dynamic processes in living cells is a continual challenge. Herein, a novel near-infrared photoluminescence probe with long lifetime was exploited for photoluminescence lifetime imaging (PLIM) based on an Iridium-alkyne complex. This probe offers benefits of desirable deep-red to NIR emission, long stokes shift, excellent cell penetration, low cytotoxicity and good resistance to photobleaching. To the best of our knowledge this is the first PLIM probe applicable to click reaction of Cu(I)-catalysed azide-alkyne cycloaddition with remarkable lifetime shifts of 414 ns before and after click reaction. The approach fully eliminates the background interference and well distinguishes the reacted probes from the unreacted probes, thus enabling the wash-free imaging of the newly synthesized proteins in single living cells. Based on the unique properties of the Iridium complexes, it is anticipated to be applied in more important issues in living cells.
[NMR paper] A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells.
J Biomol NMR. 2016 Sep 8;
Authors: Hikone Y, Hirai G, Mishima M, Inomata K, Ikeya T, Arai S, Shirakawa M, Sodeoka M, Ito Y
Abstract
Structural analyses of...
nmrlearner
Journal club
0
09-22-2016 06:31 AM
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells
A new carbamidemethyl-linked lanthanoid chelating tag for PCS NMR spectroscopy of proteins in living HeLa cells
Abstract
Structural analyses of proteins under macromolecular crowding inside human cultured cells by in-cell NMR spectroscopy are crucial not only for explicit understanding of their cellular functions but also for applications in medical and pharmaceutical sciences. In-cell NMR experiments using human cultured cells however suffer from low sensitivity, thus pseudocontact shifts from protein-tagged paramagnetic lanthanoid ions, analysed...
nmrlearner
Journal club
0
09-22-2016 06:26 AM
Hyperpolarized choline as an MR imaging molecular probe: feasibility of in vivo imaging in a rat model
From The DNP-NMR Blog:
Hyperpolarized choline as an MR imaging molecular probe: feasibility of in vivo imaging in a rat model
Friesen-Waldner, L.J., et al., Hyperpolarized choline as an MR imaging molecular probe: feasibility of in vivo imaging in a rat model. J Magn Reson Imaging, 2015. 41(4): p. 917-23.
http://www.ncbi.nlm.nih.gov/pubmed/24862837
nmrlearner
News from NMR blogs
0
10-22-2015 03:29 AM
[NMR paper] Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy.
Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy.
Non-invasive Analysis of Proteins in Living Cells Using NMR Spectroscopy.
Yakugaku Zasshi. 2015;135(3):391-8
Authors: Tochio H, Murayama S, Inomata K, Morimoto D, Ohno A, Shirakawa M
Abstract
**NMR spectroscopy enables structural analyses of proteins and has been widely used in the structural biology field in recent decades. NMR spectroscopy can be applied to proteins inside living cells, allowing characterization of their structures and dynamics in...
nmrlearner
Journal club
0
03-12-2015 10:33 AM
[NMR paper] (19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
(19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
(19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
Chemistry. 2013 Aug 6;
Authors: Ye Y, Liu X, Zhang Z, Wu Q, Jiang B, Jiang L, Zhang X, Liu M, Pielak GJ, Li C
Abstract
Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging....
nmrlearner
Journal club
0
08-08-2013 03:46 PM
[NMR paper] High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System.
J Am Chem Soc. 2013 Jan 27;
Authors: Hamatsu J, O'Donovan D, Tanaka T, Shirai T, Hourai Y, Mikawa T, Ikeya T, Mishima M, Boucher W, Smith BO, Laue ED, Shirakawa M, Ito Y
...
nmrlearner
Journal club
0
02-03-2013 10:19 AM
High-Resolution HeteronuclearMultidimensional NMRof Proteins in Living Insect Cells Using a Baculovirus Protein ExpressionSystem
High-Resolution HeteronuclearMultidimensional NMRof Proteins in Living Insect Cells Using a Baculovirus Protein ExpressionSystem
Jumpei Hamatsu, Daniel O’Donovan, Takashi Tanaka, Takahiro Shirai, Yuichiro Hourai, Tsutomu Mikawa, Teppei Ikeya, Masaki Mishima, Wayne Boucher, Brian O. Smith, Ernest D. Laue, Masahiro Shirakawa and Yutaka Ito
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja310928u/aop/images/medium/ja-2012-10928u_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/ja310928u...
nmrlearner
Journal club
0
01-27-2013 11:41 PM
On-Cell MAS NMR: PhysiologicalClues from Living Cells
On-Cell MAS NMR: PhysiologicalClues from Living Cells
Giorgia Zandomeneghi, Karin Ilg, Markus Aebi and Beat H. Meier
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307467p/aop/images/medium/ja-2012-07467p_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja307467p
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/PBl2HVolQkY
Photoluminescence Lifetime Imaging of Newly Synthesized Proteins in Living Cells with Iridium-alkyne Probe
Linear Mode
