NMR paper Photo-CIDNP NMR methods for studying protein folding.

		BioNMR > Educational resources > Journal club
[NMR paper] Photo-CIDNP NMR methods for studying protein folding.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 10:01 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Photo-CIDNP NMR methods for studying protein folding.

Photo-CIDNP NMR methods for studying protein folding.

Related Articles Photo-CIDNP NMR methods for studying protein folding.

Methods. 2004 Sep;34(1):75-87

Authors: Mok KH, Hore PJ

Chemically induced dynamic nuclear polarization (CIDNP) is a nuclear magnetic resonance phenomenon that can be used to probe the solvent-accessibility of tryptophan, tyrosine, and histidine residues in proteins by means of laser-induced photochemical reactions, resulting in significant enhancement of NMR signals. CIDNP offers good sensitivity as a surface probe of protein structure and is particularly powerful in time-resolved NMR measurements. Real-time, rapid-injection protein refolding experiments permit the observation of changes in the accessibility of specific residues during the folding process. CIDNP pulse-labeling gives information on the accessibility of residues in partially structured proteins (e.g., molten globule states) whose NMR spectra are broad and poorly resolved. Heteronuclear two-dimensional (15)N-(1)H CIDNP techniques allow identification of surface-accessible residues with improved resolution and sensitivity. These methods offer residue-specific structural and kinetic information on transient folding intermediates and other partially folded states of proteins that are not readily available from more routine NMR techniques.

PMID: 15283917 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
(1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. (1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. (1)H-Detected (13)C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR. J Am Chem Soc. 2011 May 6; Authors: Lee JH, Sekhar A, Cavagnero S NMR is a powerful yet intrinsically insensitive technique. The applicability of NMR to chemical and biological systems would be substantially extended by new approaches going beyond current signal-to-noise capabilities. Here, we exploit the large enhancements arising from (13)C photochemically induced dynamic nuclear...	nmrlearner	Journal club	0	05-10-2011 05:11 PM
1H-Detected 13C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR 1H-Detected 13C Photo-CIDNP as a Sensitivity Enhancement Tool in Solution NMR Jung Ho Lee, Ashok Sekhar and Silvia Cavagnero http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja111613c/aop/images/medium/ja-2010-11613c_0005.gif Journal of the American Chemical Society DOI: 10.1021/ja111613c http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/nDw3hSNxR80	nmrlearner	Journal club	0	05-06-2011 05:57 PM
Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy. Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy. Solid-state photo-CIDNP effect observed in phototropin LOV1-C57S by (13)C magic-angle spinning NMR spectroscopy. J Am Chem Soc. 2010 Nov 10;132(44):15542-3 Authors: Thamarath SS, Heberle J, Hore PJ, Kottke T, Matysik J Until now, the solid-state photo-CIDNP effect, discovered in 1994 by Zysmilich and McDermott, has been observed selectively in photosynthetic systems. Here we present the first observation of this effect in a...	nmrlearner	Journal club	0	03-02-2011 11:54 AM
[NMR paper] Photo-CIDNP NMR spectroscopy of a heme-containing protein. Photo-CIDNP NMR spectroscopy of a heme-containing protein. Related Articles Photo-CIDNP NMR spectroscopy of a heme-containing protein. J Magn Reson. 2005 Aug;175(2):330-5 Authors: Day IJ, Wain R, Tozawa K, Smith LJ, Hore PJ There are relatively few examples of the application of photo-CIDNP NMR spectroscopy to chromophore-containing proteins. The most likely reason for this is that simultaneous absorption of light by the photosensitiser molecule and the protein chromophore reduces the effectiveness of the photochemical reaction that produces...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applica Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applications to real-time protein folding. Related Articles Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applications to real-time protein folding. J Am Chem Soc. 2003 Oct 15;125(41):12484-92 Authors: Mok KH, Nagashima T, Day IJ, Jones JA, Jones CJ, Dobson CM, Hore PJ We describe the development and application of a novel rapid sample-mixing technique for real-time NMR (nuclear magnetic resonance) spectroscopy. The apparatus consists...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
Ph.D. student in solid-state NMR: Studying protein folding and assembly at atomic sca Ph.D. student in solid-state NMR: Studying protein folding and assembly at atomic scale Description of the Faculty / Research group The Faculty of Science consists of six departments: Biology, Pharmaceutical Sciences, Information and Computing Sciences, Physics and Astronomy, Mathematics and Chemistry. The Faculty is home to 3500 students and nearly 2000 staff and is internationally renowned for the quality of its research. The NMR Research Group is part of the Chemistry Department (Universiteit Utrecht fac. Scheikunde) and belongs to the Bijvoet Center for Biomolecular...	markber	Job marketplace	0	09-11-2010 07:15 PM
[NMR paper] Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Related Articles Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Glycoconj J. 1997 Jun;14(4):531-4 Authors: Siebert HC, Kaptein R, Beintema JJ, Soedjanaatmadja UM, Wright CS, Rice A, Kleineidam RG, Kruse S, Schauer R, Pouwels PJ, Kamerling JP, Gabius HJ, Vliegenthart JF The side chains of tyrosine, tryptophan and histidine are able to produce CIDNP (Chemically Induced Dynamic Nuclear Polarization) signals after laser irradiation in the...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Related Articles Carbohydrate-protein interaction studies by laser photo CIDNP NMR methods. Glycoconj J. 1997 Jun;14(4):531-4 Authors: Siebert HC, Kaptein R, Beintema JJ, Soedjanaatmadja UM, Wright CS, Rice A, Kleineidam RG, Kruse S, Schauer R, Pouwels PJ, Kamerling JP, Gabius HJ, Vliegenthart JF The side chains of tyrosine, tryptophan and histidine are able to produce CIDNP (Chemically Induced Dynamic Nuclear Polarization) signals after laser irradiation in the...	nmrlearner	Journal club	0	08-22-2010 03:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off