Related ArticlesPhospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: the effects of a peripheral protein on collective lipid fluctuations.
Solid State Nucl Magn Reson. 1997 Mar;8(1):55-64
Authors: Pinheiro TJ, Duer MJ, Watts A
The dynamics of the glycerol headgroup of dioleoylphosphatidylglycerol (DOPG) in hydrated bilayers were studied by 2H and 31P NMR spectroscopy, and the effects of binding a peripheral protein, cytochrome c, were evaluated. The fast headgroup segmental motions (tau c, 10(-10)-(-13) s) of DOPG in fully hydrated bilayers were not affected upon binding of cytochrome c, as evaluated by the spin-lattice (T1) relaxation of deuterons in the DOPG glycerol headgroup. In contrast, the spin-spin (T2e) relaxation is strongly affected, indicating that slow cooperative bilayer motions (tau c, 10(-3)-10(-6) s) are enhanced upon the interaction with cytochrome c, 2H and 31P NMR spectral lineshape analysis reveal details of the nature of these motions. The importance of these effects are discussed in terms of a possible mechanism for modulating membrane-associated processes.
[NMR paper] High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
Related Articles High-resolution 31p field cycling NMR as a probe of phospholipid dynamics.
J Am Chem Soc. 2004 Oct 27;126(42):13765-77
Authors: Roberts MF, Redfield AG
We have used high-resolution field-cycling 31P NMR spectroscopy to measure spin-lattice relaxation rates (R1 = 1/T1) of multicomponent phospholipid vesicle and micelle samples over a large field range, from 0.1 to 11.7 T. The shape of the curve for R1 as a function of field and a model-free analysis were...
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[NMR paper] The stability of the cytochrome c scaffold as revealed by NMR spectroscopy.
The stability of the cytochrome c scaffold as revealed by NMR spectroscopy.
Related Articles The stability of the cytochrome c scaffold as revealed by NMR spectroscopy.
J Inorg Biochem. 2004 May;98(5):814-23
Authors: Berners-Price SJ, Bertini I, Gray HB, Spyroulias GA, Turano P
NMR spectroscopy was used to study the effect of guanidinium chloride on the unfolding of horse heart and yeast iso-1 cytochrome c under mild alkaline conditions. The structural changes on the horse heart protein were detected through NOESY (Nuclear Overhauser Effect...
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[NMR paper] Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NM
Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments.
Related Articles Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments.
BMC Biochem. 2003 Dec 23;4:18
Authors: Fischer M, Schott AK, Kemter K, Feicht R, Richter G, Illarionov B, Eisenreich W, Gerhardt S, Cushman M, Steinbacher S, Huber R, Bacher A
BACKGROUND: Riboflavin synthase catalyzes the transformation of 6,7-dimethyl-8-ribityllumazine into riboflavin in...
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[NMR paper] Conformation and backbone dynamics of bacteriorhodopsin revealed by (13)C-NMR.
Conformation and backbone dynamics of bacteriorhodopsin revealed by (13)C-NMR.
Related Articles Conformation and backbone dynamics of bacteriorhodopsin revealed by (13)C-NMR.
Biochim Biophys Acta. 2000 Aug 30;1460(1):39-48
Authors: Saitô H, Tuzi S, Yamaguchi S, Tanio M, Naito A
It is demonstrated here how the secondary structure and dynamics of transmembrane helices, as well as surface residues, such as interhelical loops and N- or C-terminus of bacteriorhodopsin (bR) in purple membrane, can be determined at ambient temperature based on very...
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[NMR paper] Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H a
Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: the effects of a peripheral protein on collective lipid fluctuations.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: the effects of a peripheral protein on collective lipid fluctuations.
Solid State Nucl Magn Reson. 1997 Mar;8(1):55-64
Authors: Pinheiro TJ, Duer MJ, Watts A
The...
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[NMR paper] Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilaye
Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Related Articles Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Biochemistry. 1994 Mar 8;33(9):2451-8
Authors: Pinheiro TJ, Watts A
Phosphorus-31 NMR has been used to investigate the interaction of cytochrome c with bilayers of the anionic lipids dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylserine (DOPS), and diacylphosphatidylinositol...
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[NMR paper] Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilaye
Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Related Articles Lipid specificity in the interaction of cytochrome c with anionic phospholipid bilayers revealed by solid-state 31P NMR.
Biochemistry. 1994 Mar 8;33(9):2451-8
Authors: Pinheiro TJ, Watts A
Phosphorus-31 NMR has been used to investigate the interaction of cytochrome c with bilayers of the anionic lipids dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylserine (DOPS), and diacylphosphatidylinositol...
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[NMR paper] Phospholipid phase transitions as revealed by NMR.
Phospholipid phase transitions as revealed by NMR.
Related Articles Phospholipid phase transitions as revealed by NMR.
Chem Phys Lipids. 1991 Mar;57(2-3):195-211
Authors: Watts A, Spooner PJ
Aqueous dispersions of phospholipids can adopt a range of polymorphic phases which include bilayer and non-bilayer forms. Within the bilayer form, laterally separated phases may be induced as a result of surface electrostatic associations, thermotropic behaviour, lipid-protein interactions or because of molecular mismatch between chemically distinct...
Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H a
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