Related ArticlesA peroxidative model of human erythrocyte intracellular Ca2+ changes with in vivo cell aging: measurement by 19F-NMR spectroscopy.
Biochim Biophys Acta. 1995 Jan 25;1270(1):52-7
Authors: Aiken NR, Galey WR, Satterlee JD
Numerous changes occur with human erythrocyte aging in vivo, including an increase in free ionic intracellular calcium concentration ([Ca2+]i) (N.R. Aiken et al. (1992) Biochim. Biophys. Acta 1136, 155-160). An attractive hypothesis of cell aging suggests that oxidative stress is responsible for many age-related changes. To determine whether oxidative stress leads to increased intracellular Ca2+ concentrations, we used the fluorinated calcium probe 5,5'-difluoroBAPTA and fluorine nuclear magnetic resonance spectroscopy (19F-NMR) to measure [Ca2+]i following mild hydrogen peroxide (H2O2) stress to young red cells. Cells were separated using density centrifugation, exposed to 815 microM H2O2, loaded with the calcium probe, and [Ca2+]i measured. Intracellular [Ca2+] increased from 62 nM (+/- 4, S.E.) in untreated young cells to 173 nM (+/- 11) in peroxide treated cohort young cells. This value approached our previously reported [Ca2+]i of 221 nM (+/- 25) in old human erythrocytes. Pretreatment of young cells with (a) cobalt, which blocks Ca2+ influx through calcium channels, or (b) carbon monoxide, which prevents methemoglobin formation, inhibited the peroxide-induced increase in ionic intracellular calcium. These findings are consistent with the hypothesis that oxidative stress of erythrocytes contributes to the increased [Ca2+]i found in senescent cells, and that this is due to increased membrane Ca2+ leak resulting from oxidatively induced methemoglobin-cytoskeletal protein crosslinking.
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Bacterial expression, purification, and model membrane reconstitution of the transmembrane and cytoplasmic domains of the human APP binding protein LR11/SorLA for NMR studies.
Protein Expr Purif. 2011 Feb 11;
Authors: Wang X, Gill Jr RL, Zhu Q, Tian F
LR11 (SorLA) is a recently identified neuronal protein that interacts with amyloid precursor protein (APP), a central player...
[NMR paper] NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: e
NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix.
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Biochemistry. 2002 Mar 19;41(11):3596-604
Authors: Chung DA, Zuiderweg ER, Fowler CB, Soyer OS, Mosberg HI, Neubig RR
A major, unresolved question in signal transduction by G protein coupled receptors (GPCRs) is to understand how, at atomic resolution, a GPCR activates a G protein. A...
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[NMR paper] A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of s
A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles A model for human cytochrome P450 2D6 based on homology modeling and NMR studies of substrate binding.
Biochemistry. 1996 Apr 9;35(14):4540-50
Authors: Modi S, Paine MJ, Sutcliffe MJ, Lian LY, Primrose WU, Wolf CR, Roberts GC
The cytochrome P450 responsible for the debrisoquine/sparteine polymorphism (P450 2D6) has been produced in large...
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[NMR paper] NMR study of the selective inhibition of water permeability of rat erythrocyte membra
NMR study of the selective inhibition of water permeability of rat erythrocyte membrane.
Related Articles NMR study of the selective inhibition of water permeability of rat erythrocyte membrane.
Biosci Rep. 1995 Feb;15(1):55-63
Authors: Petcu I, Lupu M, Grosescu R
The inhibition of water diffusion across the rat erythrocyte membrane was studied by NMR using two basically different types of inhibitory agents: PCMB and in vivo irradiation. The contribution of lipid and protein to water permeability revealed the inhibitory effect of each pathway....
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[NMR paper] A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport prot
A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles A 35Cl and 37Cl NMR study of chloride binding to the erythrocyte anion transport protein.
Biophys Chem. 1991 Jul;40(3):329-37
Authors: Price WS, Kuchel PW, Cornell BA
Band 3, the erythrocyte anion transport protein, mediates the one-for-one exchange of bicarbonate and chloride ions across the membrane and consequently plays an...
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[NMR paper] 19F-NMR study of the effect of lead on intracellular free calcium in human platelets.
19F-NMR study of the effect of lead on intracellular free calcium in human platelets.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles 19F-NMR study of the effect of lead on intracellular free calcium in human platelets.
Biochim Biophys Acta. 1991 May 17;1092(3):341-6
Authors: Dowd TL, Gupta RK
Lead has been shown to affect calcium homeostasis. However, there is no prior evidence to indicate an effect of low concentrations of lead in the environment (approximately 1...
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[NMR paper] 77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
Related Articles 77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
J Biol Chem. 1991 Mar 15;266(8):4804-9
Authors: Gettins P, Crews BC
Lambs, maintained on a selenium-deficient diet supplemented with 94 atom % Na2 27SeO3, have been used as a source of 77Se-enriched erythrocyte glutathione peroxidase. After 5 months on this diet, the percentage of selenium in the enzyme derived from the supplement had reached 88%. From each...
A peroxidative model of human erythrocyte intracellular Ca2+ changes with in vivo cel
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