Solid-state Nuclear Magnetic Resonance (NMR) in combination with magnetically aligned discoidal lipid mimics allows for studying the conformations of membrane proteins in planar, lipid-rich bilayer environments and at the physiological temperature. We have recently demonstrated the general applicability of macrodiscs composed of DMPC lipids and peptoid belts, which yield magnetic alignment and NMR spectroscopic resolution comparable or superior to detergent-containing bicelles. Here we report on...
[NMR paper] Aligned peptoid-based macrodiscs for structural studies of membrane proteins by oriented-sample nmr
ALIGNED PEPTOID-BASED MACRODISCS FOR STRUCTURAL STUDIES OF MEMBRANE PROTEINS BY ORIENTED-SAMPLE NMR
Development of a robust, uniform, and magnetically orientable lipid mimetics will undoubtedly advance solid-state NMR of macroscopically aligned membrane proteins. Here we report on a novel lipid membrane mimetic based on peptoid belts. The peptoids composed of 15 residues were synthesized by alternating N-(2-carboxyethyl)glycine with N-(2-phenethyl)glycine residues at the 2:1 molar ratio. The chemically synthesized peptoids possess much lower degree of polydispersity as compared to...
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08-04-2022 01:03 AM
[NMR paper] Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Related Articles Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins.
Biophys J. 2018 Jun 15;:
Authors: Radoicic J, Park SH, Opella SJ
Abstract
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30*nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer...
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06-20-2018 08:56 PM
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins
Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins
Publication date: Available online 15 June 2018
Source:Biophysical Journal</br>
Author(s): Jasmina Radoicic, Sang Ho Park, Stanley J. Opella</br>
Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30*nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under...
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06-16-2018 07:20 AM
[NMR paper] Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Related Articles Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase.
Biophys J. 2014 Apr 15;106(8):1559-69
Authors: Murray DT, Li C, Gao FP, Qin H, Cross TA
Abstract
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same...
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04-18-2014 01:35 PM
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Membrane Protein Structural Validation by Oriented Sample Solid-State NMR: Diacylglycerol Kinase
Publication date: 15 April 2014
Source:Biophysical Journal, Volume 106, Issue 8</br>
Author(s): Dylan*T. Murray , Conggang Li , F.*Philip Gao , Huajun Qin , Timothy*A. Cross</br>
The validation of protein structures through functional assays has been the norm for many years. Functional assays perform this validation for water-soluble proteins very well, but they need to be performed in the same environment as that used for the structural analysis. This is difficult...
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
Long-Term-Stable Ether-Lipid vs Conventional Ester-Lipid Bicelles in Oriented Solid-State NMR: Altered Structural Information in Studies of Antimicrobial Peptides.
J Phys Chem B. 2011 Feb 10;
Authors: Bertelsen K, Vad B, Nielsen EH, Hansen SK, Skrydstrup T, Otzen DE, Vosegaard T, Nielsen NC
Recently, ether lipids have been introduced as long-term stable alternatives to the more natural,...
Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR
Linear Mode
