Labeling of biomolecules with a paramagnetic probe for nuclear magnetic resonance (NMR) spectroscopy enables determining long-range distance restraints, which are otherwise not accessible by classically used dipolar coupling-based NMR approaches. Distance restraints derived from paramagnetic relaxation enhancements (PREs) can facilitate the structure determination of large proteins and protein complexes. We herein present the site-directed labeling of the large oligomeric bacterial DnaB helicase...
[NMR paper] Paramagnetic Solid-state NMR to Localize the Metal-Ion Cofactor in an Oligomeric DnaB Helicase
Paramagnetic Solid-state NMR to Localize the Metal-Ion Cofactor in an Oligomeric DnaB Helicase
Paramagnetic metal ions can be inserted into ATP-fueled motor proteins by exchanging the diamagnetic Mg 2+ cofactor with Mn 2+ or Co 2+ . Then, paramagnetic relaxation enhancement (PRE) or pseudo-contact shifts (PCSs) can be measured to report on the localization of the metal ion within the protein. We determine the metal position in the oligomeric bacterial DnaB helicase from Helicobacter pylori complexed with the transition-state ATP-analogue ADP:AlF 4 - and single-stranded DNA using...
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04-07-2021 02:21 AM
[NMR paper] Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin?
Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin?
ConspectusMembrane proteins mediate a plethora of cellular functions and represent important targets for drug development. Unlike soluble proteins, membrane proteins require native-like environments to fold correctly and be active. Therefore, modern structural biology techniques have aimed to determine the structure and dynamics of these membrane proteins at physiological temperature and in liquid crystalline lipid bilayers. With the flourishing of new NMR methodologies and improvements in...
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03-04-2021 12:41 PM
New limits of sensitivity of site-directed spin labeling electron paramagnetic resonance for membrane proteins #DNPNMR
From The DNP-NMR Blog:
New limits of sensitivity of site-directed spin labeling electron paramagnetic resonance for membrane proteins #DNPNMR
A nice overview how spinlabels can be used for structural biology studies. This includes pulsed EPR techniques such as PELDOR (DEER) and ODNP spectroscopy.
Bordignon, Enrica, and Stephanie Bleicken. “New Limits of Sensitivity of Site-Directed Spin Labeling Electron Paramagnetic Resonance for Membrane Proteins.” Biochimica et Biophysica Acta (BBA) - Biomembranes 1860, no. 4 (April 2018): 841–53. https://doi.org/10.1016/j.bbamem.2017.12.009.
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03-24-2020 04:01 AM
Protein Displacement by Herpes Helicase-Primase andthe Key Role of UL42 during Helicase-Coupled DNA Synthesis by theHerpes Polymerase
Protein Displacement by Herpes Helicase-Primase andthe Key Role of UL42 during Helicase-Coupled DNA Synthesis by theHerpes Polymerase
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b01128/20170518/images/medium/bi-2016-011285_0014.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b01128
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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05-20-2017 07:21 AM
[NMR paper] Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.
Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.
Related Articles Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase.
Biomol NMR Assign. 2015 Aug 18;
Authors: Wiegand T, Gardiennet C, Ravotti F, Bazin A, Kunert B, Lacabanne D, Cadalbert R, Güntert P, Terradot L, Böckmann A, Meier BH
Abstract
We present solid-state NMR assignments of the N-terminal domain of the DnaB helicase from Helicobacter pylori (153 residues) in its microcrystalline form. We use a sequential...
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08-19-2015 03:24 PM
Spin-Transfer Pathwaysin Paramagnetic Lithium Transition-MetalPhosphates from Combined Broadband Isotropic Solid-State MAS NMR Spectroscopyand DFT Calculations
Spin-Transfer Pathwaysin Paramagnetic Lithium Transition-MetalPhosphates from Combined Broadband Isotropic Solid-State MAS NMR Spectroscopyand DFT Calculations
Raphae?le J. Cle?ment, Andrew J. Pell, Derek S. Middlemiss, Fiona C. Strobridge, Joel K. Miller, M. Stanley Whittingham, Lyndon Emsley, Clare P. Grey and Guido Pintacuda
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja306876u/aop/images/medium/ja-2012-06876u_0007.gif
Journal of the American Chemical Society
DOI: 10.1021/ja306876u...
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10-10-2012 03:14 PM
[NMR paper] Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectrosco
Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectroscopy.
Biochem Biophys Res Commun. 1997 Feb 3;231(1):126-30
Authors: Miles CS, Weigelt J, Stamford NP, Dammerova N, Otting G, Dixon NE
Two separate N-terminal fragments of the 470-amino-acid Escherichia coli DnaB helicase, comprising residues 1-142 and...
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08-22-2010 03:31 PM
[NMR paper] Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectrosco
Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Precise limits of the N-terminal domain of DnaB helicase determined by NMR spectroscopy.
Biochem Biophys Res Commun. 1997 Feb 3;231(1):126-30
Authors: Miles CS, Weigelt J, Stamford NP, Dammerova N, Otting G, Dixon NE
Two separate N-terminal fragments of the 470-amino-acid Escherichia coli DnaB helicase, comprising residues 1-142 and...