[NMR paper] Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.
Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.
Related ArticlesPacking Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.
Biomacromolecules. 2019 10 14;20(10):3884-3894
Authors: Asakura T, Nishimura A, Aoki A, Naito A
Abstract
Packing structures of polyalanine regions, which are considered to be the reason for the extremely high strength of spider dragline silks, were studied using a series of sequential peptides: (Glu)4GlyGlyLeuGlyGlyGlnGlyAlaGly(Ala)nGlyGlyAlaGl yGlnGlyGlyTyrGlyGly(Glu)4 (n = 3-8) using 13C solid-state NMR spectroscopy. The conformations of (Ala)n in the freeze-dried peptides changed gradually with increasing n from random coils to ?-helices with partial antiparallel ?-sheet (AP-?) structures. Conversely, all the insolubilized peptides, n = 6-8 after low-pH treatment and n = 4-8 after formic acid/methanol treatment, formed AP-? structures with significant amounts of staggered packing arrangements. These results are different from previously obtained results for pure alanine oligopeptides, that is, AP-? (Ala)n formed rectangular packing for less than n = 6 but staggered packings for n >= 7. The 13C-labeled peptides were also used to confirm the staggered packing arrangements from NMR dynamics. Furthermore, a MD simulation supported the observed results.
[NMR paper] Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Packing arrangement of 13C selectively labeled sequence model peptides of Samia cynthia ricini silk fibroin fibers studied by solid-state NMR.
Phys Chem Chem Phys. 2017 May 24;19(20):13379-13386
Authors: Asakura T, Miyazawa K, Tasei Y, Kametani S, Nakazawa Y, Aoki A, Naito A...
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[NMR paper] Distinct solvent- and temperature-dependent packing arrangements of anti-parallel ?-sheet polyalanines studied with solid-state 13C NMR and MD simulation.
Distinct solvent- and temperature-dependent packing arrangements of anti-parallel ?-sheet polyalanines studied with solid-state 13C NMR and MD simulation.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Distinct solvent- and temperature-dependent packing arrangements of anti-parallel ?-sheet polyalanines studied with solid-state 13C NMR and MD simulation.
Phys Chem Chem Phys. 2017 Aug 09;19(31):20829-20838
Authors: Kametani S, Tasei Y, Nishimura A, Asakura T
...
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[NMR paper] Solid-state NMR spectroscopy of the HIV gp41 membrane fusion protein supports intermolecular antiparallel ? sheet fusion peptide structure in the final six-helix bundle state.
Solid-state NMR spectroscopy of the HIV gp41 membrane fusion protein supports intermolecular antiparallel ? sheet fusion peptide structure in the final six-helix bundle state.
Related Articles Solid-state NMR spectroscopy of the HIV gp41 membrane fusion protein supports intermolecular antiparallel ? sheet fusion peptide structure in the final six-helix bundle state.
J Mol Biol. 2013 Nov 15;
Authors: Sackett K, Nethercott MJ, Zheng Z, Weliky DP
Abstract
The HIV gp41 protein catalyzes fusion between viral and target cell membranes. Although...
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Solid-state NMR spectroscopy of the HIV gp41 membrane fusion protein supports intermolecular antiparallel ? sheet fusion peptide structure in the final six-helix bundle state
Solid-state NMR spectroscopy of the HIV gp41 membrane fusion protein supports intermolecular antiparallel ? sheet fusion peptide structure in the final six-helix bundle state
Publication date: Available online 16 November 2013
Source:Journal of Molecular Biology</br>
Author(s): Kelly Sackett , Matthew J. Nethercott , Zhaoxiong Zheng , David P. Weliky</br>
The HIV gp41 protein catalyzes fusion between viral and target cell membranes. Although the ~20-residue N-terminal fusion peptide (FP) region is critical for fusion, the structure of this region is not...
[NMR paper] NMR characterization of native liquid spider dragline silk from Nephila edulis.
NMR characterization of native liquid spider dragline silk from Nephila edulis.
Related Articles NMR characterization of native liquid spider dragline silk from Nephila edulis.
Biomacromolecules. 2004 May-Jun;5(3):834-9
Authors: Hronska M, van Beek JD, Williamson PT, Vollrath F, Meier BH
Solid spider dragline silk is well-known for its mechanical properties. Nonetheless a detailed picture of the spinning process is lacking. Here we report NMR studies on the liquid silk within the wide sac of the major ampullate (m.a.) gland from the spider...
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[NMR paper] 13C NMR of Nephila clavipes major ampullate silk gland.
13C NMR of Nephila clavipes major ampullate silk gland.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles 13C NMR of Nephila clavipes major ampullate silk gland.
Biophys J. 1996 Dec;71(6):3442-7
Authors: Hijirida DH, Do KG, Michal C, Wong S, Zax D, Jelinski LW
The major ampullate glands of the spider Nephila clavipes contain approximately 0.2 microliter each...
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[NMR paper] Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Supercontracted spider dragline silk: a solid-state NMR study of the local structure.
Int J Biol Macromol. 1999 Mar-Apr;24(2-3):173-8
Authors: van Beek JD, Kümmerlen J, Vollrath F, Meier BH
The local structure of supercontracted dragline silk from the spider Nephila madagascariensis was investigated by solid-state nuclear magnetic...
Packing Structure of Antiparallel ?-Sheet Polyalanine Region in a Sequential Model Peptide of Nephila clavipes Dragline Silk Studied Using 13C Solid-State NMR and MD Simulation.
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