[NMR paper] Packing Arrangements and Inter-Sheet Interaction of Alanine Oligopeptides as Revealed by Relaxation Parameters Obtained From High-Resolution (13)C Solid-State NMR.
Packing Arrangements and Inter-Sheet Interaction of Alanine Oligopeptides as Revealed by Relaxation Parameters Obtained From High-Resolution (13)C Solid-State NMR.
Related ArticlesPacking Arrangements and Inter-Sheet Interaction of Alanine Oligopeptides as Revealed by Relaxation Parameters Obtained From High-Resolution (13)C Solid-State NMR.
J Phys Chem B. 2017 Sep 05;:
Authors: Naito A, Tasei Y, Nishimura A, Asakura T
Abstract
Alanine oligopeptides provide a key structure of the crystalline domains of the silks from spiders and wild silkworm, and also the sequences included in proteins such as antifreeze proteins and amyloids. In this paper, the local dynamics of alanine oligopeptides, (Ala)3, (Ala)4 and (Ala)6 were examined by high-resolution (13)C solid-state NMR. The (13)C spin-lattice relaxation times (T1's) for the C?4 carbons of antiparallel (AP)-?-sheet (Ala)4 significantly prolonged and the correlation time was estimated as 3.6 x 10(-11) sec which was shorter than those of other carbons in the AP-?-sheet (Ala)4 (2.8 x 10(-10) sec). The T1 values for the C? carbons of (Ala)6 showed significantly longer correlation time (8.8 x 10(-9) sec) than those of AP-?-sheet (Ala)4. It is thus revealed that AP-?-sheet (Ala)6 exhibited stronger inter-sheet interaction than those of AP-?-sheet (Ala)4. The (13)C spin-spin relaxation times (T2's) for the C?4 carbons showed longer than those of the other C?1-3 carbons of AP-?-sheet (Ala)4. T2 values of C? carbons reflect the slow time-scale (~70 kHz) backbone motions. The C-terminal forms strong hydrogen bonds with water molecules and thus the backbone motion is slower than ~70 kHz, while the central backbone motions are faster than ~70 kHz in AP-?-sheet (Ala)4.
PMID: 28872862 [PubMed - as supplied by publisher]
[NMR paper] Synergic Effect of Active Sites in Zinc-Modified ZSM-5 Zeolites as Revealed by High-Field Solid-State NMR Spectroscopy
Synergic Effect of Active Sites in Zinc-Modified ZSM-5 Zeolites as Revealed by High-Field Solid-State NMR Spectroscopy
Understanding the nature of active sites in metal-supported catalysts is of great importance towards establishing their structure–property relationships. The outstanding catalytic performance of metal-supported catalysts is frequently ascribed to the synergic effect of different active sites, which is however not well spectroscopically characterized. Herein, we report the direct detection of surface Zn species and 1H–67Zn internuclear interaction between Zn2+ ions and...
nmrlearner
Journal club
0
11-19-2016 08:35 PM
[NMR paper] Difference in the structures of alanine tri- and tetra-peptides with antiparallel ?-sheet assessed by X-ray diffraction, solid-state NMR and chemical shift calculations by GIPAW.
Difference in the structures of alanine tri- and tetra-peptides with antiparallel ?-sheet assessed by X-ray diffraction, solid-state NMR and chemical shift calculations by GIPAW.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Difference in the structures of alanine tri- and tetra-peptides with antiparallel ?-sheet assessed by X-ray diffraction, solid-state NMR and chemical shift calculations by GIPAW.
Biopolymers. 2014 Jan;101(1):13-20
Authors: ...
nmrlearner
Journal club
0
04-23-2016 09:24 PM
[NMR paper] Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement.
Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement.
Related Articles Ligand orientation in a membrane-embedded receptor site revealed by solid-state NMR with paramagnetic relaxation enhancement.
Org Biomol Chem. 2015 Jan 13;
Authors: Whittaker CA, Patching SG, Esmann M, Middleton DA
Abstract
NMR relaxation enhancement by paramagnetic metals provides powerful restraints on the three-dimensional structures of proteins in solution, and this approach has...
nmrlearner
Journal club
0
01-15-2015 06:10 PM
[NMR paper] Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.
Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Covariance spectroscopy in high-resolution multi-dimensional solid-state NMR.
J Magn Reson. 2014 Feb;239:57-60
Authors: Lin EC, Opella SJ
Abstract
Covariance spectroscopy (COV), a statistical method that provides increased sensitivity, can be applied to two-dimensional high-resolution solid-state NMR...
nmrlearner
Journal club
0
10-07-2014 02:31 PM
[NMR paper] High-resolution solid-state MAS NMR of proteins-Crh as an example.
High-resolution solid-state MAS NMR of proteins-Crh as an example.
Related Articles High-resolution solid-state MAS NMR of proteins-Crh as an example.
Magn Reson Chem. 2007 Dec;45 Suppl 1:S24-31
Authors: Böckmann A
Abstract
Solid-state NMR spectroscopy provides unique possibilities for the structural investigation of insoluble molecules at the atomic level. Recent efforts aim at solving the complete structures of biological macromolecules using high-resolution magic angle spinning NMR. Structurally homogenous samples of -labeled...
nmrlearner
Journal club
0
04-03-2013 08:22 PM
[NMR paper] Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.rsc.org-images-entities-char_z_RSClogo.gif Related Articles Determination of accurate 1H positions of an alanine tripeptide with anti-parallel and parallel ?-sheet structures by high resolution 1H solid state NMR and GIPAW chemical shift calculation.
Chem Commun (Camb). 2012 Nov 25;48(91):11199-201
...
nmrlearner
Journal club
0
03-30-2013 12:59 PM
[NMR paper] A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
Related Articles A General Method for Constructing Atomic-Resolution RNA Ensembles using NMR Residual Dipolar Couplings: The Basis for Inter-helical Motions Revealed.
J Am Chem Soc. 2013 Mar 8;
Authors: Salmon L, Bascom GD, Andricioaei I, Al-Hashimi HM
Abstract
The ability to modulate alignment and measure multiple independent sets of NMR residual dipolar couplings (RDCs) has made it possible...
nmrlearner
Journal club
0
03-12-2013 07:09 PM
[NMR paper] Towards high-resolution solid-state NMR on large uniformly 15N- and [13C,15N]-labeled
Towards high-resolution solid-state NMR on large uniformly 15N- and -labeled membrane proteins in oriented lipid bilayers.
Related Articles Towards high-resolution solid-state NMR on large uniformly 15N- and -labeled membrane proteins in oriented lipid bilayers.
J Biomol NMR. 2002 Mar;22(3):225-47
Authors: Vosegaard T, Nielsen NC
Based on exact numerical simulations, taking into account isotropic and conformation-dependent anisotropic nuclear spin interactions, we systematically analyse the prospects for high-resolution solid-state NMR on...
Packing Arrangements and Inter-Sheet Interaction of Alanine Oligopeptides as Revealed by Relaxation Parameters Obtained From High-Resolution (13)C Solid-State NMR.
Linear Mode
