Optimized selection of the slow-relaxing components of single-quantum 13C magnetization in 13CH3 methyl groups of proteins using acute (
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Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins - SelectScience
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Application Note: Isotope Labeling of Alanine Methyl Groups for NMR Studies of High-Molecular-Weight Proteins
SelectScience
Labeling schemes commonly employed for NMR investigations of high-molecular-weight proteins utilize selective incorporation of protons and 13C isotopes into methyl groups of Ileδ1, Leuδ and Valγ side-chains in a highly deuterated environment (commonly ...
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08-08-2017 10:10 AM
Longitudinal relaxation optimized amide 1 H-CEST experiments for studying slow chemical exchange processes in fully protonated proteins
Longitudinal relaxation optimized amide 1 H-CEST experiments for studying slow chemical exchange processes in fully protonated proteins
Abstract
Chemical Exchange Saturation Transfer (CEST) experiments are increasingly used to study slow timescale exchange processes in biomolecules. Although 15N- and 13C-CEST have been the approaches of choice, the development of spin state selective 1H-CEST pulse sequences that separate the effects of chemical and dipolar exchange significantly increases the utility of 1H-based experiments. Pulse schemes have been...
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03-30-2017 06:42 PM
Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection
Off-resonance rotating-frame relaxation dispersion experiment for 13C in aromatic side chains using L-optimized TROSY-selection
Abstract
Protein dynamics on the microsecondâ??millisecond time scales often play a critical role in biological function. NMR relaxation dispersion experiments are powerful approaches for investigating biologically relevant dynamics with site-specific resolution, as shown by a growing number of publications on enzyme catalysis, protein folding, ligand binding, and allostery. To date, the majority of studies has probed the...
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06-19-2014 10:21 PM
Observation and Relaxation Properties of Individual Fast-Relaxing Proton Transitions in [13CH3]-Methyl-Labeled, Deuterated Proteins
Observation and Relaxation Properties of Individual Fast-Relaxing Proton Transitions in -Methyl-Labeled, Deuterated Proteins
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Hechao Sun, Vitali Tugarinov</br>
A pair of NMR experiments is developed for separation of individual fast-relaxing transitions in 13CH3 methyl groups of methyl-protonated, highly deuterated proteins, and the measurement of their relaxation rates. Intra-methyl 1H-1H/1H-13C dipole-dipole cross-correlated spin relaxation that differentiates the rates of the fast-relaxing transitions...
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03-09-2012 09:16 AM
Observation and Relaxation Properties of Individual Fast-Relaxing Proton Transitions in [CH3]-Methyl-Labeled, Deuterated Proteins
Observation and Relaxation Properties of Individual Fast-Relaxing Proton Transitions in -Methyl-Labeled, Deuterated Proteins
Publication year: 2012
Source: Journal of Magnetic Resonance, Available online 2 March 2012</br>
Hechao*Sun, Vitali*Tugarinov</br>
A pair of NMR experiments is developed for separation of individual fast-relaxing transitions inCH3methyl groups of methyl-protonated, highly deuterated proteins, and the measurement of their relaxation rates. Intra-methylH-H/H-C dipole-dipole cross-correlated spin relaxation that differentiates the rates of the fast-relaxing...
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03-06-2012 06:04 AM
An optimized isotopic labelling strategy of isoleucine-?(2) methyl groups for solution NMR studies of high molecular weight proteins.
An optimized isotopic labelling strategy of isoleucine-?(2) methyl groups for solution NMR studies of high molecular weight proteins.
An optimized isotopic labelling strategy of isoleucine-?(2) methyl groups for solution NMR studies of high molecular weight proteins.
Chem Commun (Camb). 2011 Jul 26;
Authors: Ayala I, Hamelin O, Amero C, Pessey O, Plevin MJ, Gans P, Boisbouvier J
An efficient synthetic route is proposed to produce 2-hydroxy-2-ethyl-3-oxobutanoate for the specific labelling of Ile methyl-?(2) groups in proteins. The (2)H,...
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07-28-2011 10:51 AM
[NMR paper] Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic aci
Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids.
Related Articles Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids.
J Am Chem Soc. 2004 Sep 1;126(34):10560-70
Authors: Miclet E, Williams Jr DC, Clore GM, Bryce DL, Boisbouvier J, Bax A
A large fraction of hydrogens in proteins and nucleic acids is of the methylene type. Their detailed study, however, in terms of structure and dynamics by NMR spectroscopy is hampered by their fast relaxation properties, which give...
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11-24-2010 10:01 PM
[NMR paper] Slow internal dynamics in proteins: application of NMR relaxation dispersion spectros
Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme.
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J Am Chem Soc. 2002 Feb 20;124(7):1443-51
Authors: Mulder FA, Hon B, Mittermaier A, Dahlquist FW, Kay LE
Recently developed carbon transverse relaxation dispersion experiments (Skrynnikov, N. R.; et al. J. Am. Chem. Soc. 2001, 123, 4556-4566) were...
Optimized selection of slow-relaxing 13 C transitions in methyl groups of proteins: application to relaxation dispersion
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