Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system
Methyl-selective isotope labeling using α-ketoisovalerate for the yeast Pichia pastoris recombinant protein expression system
Abstract
Methyl-detected NMR spectroscopy is a useful tool for investigating the structures and interactions of large macromolecules such as membrane proteins. The procedures for preparation of methyl-specific isotopically-labeled proteins were established for the Escherichia coli (E. coli) expression system, but typically it is not feasible to express eukaryotic proteins using E. coli. The Pichia pastoris (P. pastoris)...
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06-05-2018 01:31 PM
[ASAP] Single-Molecule Imaging Reveals Conformational Manipulation of Holliday Junction DNA by the Junction Processing Protein RuvA
Single-Molecule Imaging Reveals Conformational Manipulation of Holliday Junction DNA by the Junction Processing Protein RuvA
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00404/20180524/images/medium/bi-2018-00404a_0006.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00404
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05-25-2018 12:07 PM
Deuteration and selective labeling of alanine methyl groups of β 2 -adrenergic receptor expressed in a baculovirus-insect cell expression system
Deuteration and selective labeling of alanine methyl groups of β 2 -adrenergic receptor expressed in a baculovirus-insect cell expression system
Abstract
G protein-coupled receptors (GPCRs) exist in equilibrium between multiple conformations, and their populations and exchange rates determine their functions. However, analyses of the conformational dynamics of GPCRs in lipid bilayers are still challenging, because methods for observations of NMR signals of large proteins expressed in a baculovirus-insect cell expression system (BVES) are limited....
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03-08-2018 01:24 PM
A quasi-optical and corrugated waveguide microwave transmission system for simultaneous dynamic nuclear polarization NMR on two separate 14.1 T spectrometers #DNPNMR
From The DNP-NMR Blog:
A quasi-optical and corrugated waveguide microwave transmission system for simultaneous dynamic nuclear polarization NMR on two separate 14.1 T spectrometers #DNPNMR
Gyrotrons typically generate much more microwave power than needed in a DNP-NMR experiment. This article describes a very nice way how to share the microwave power generated by a single gyrotron between two NMR experiments.
Dubroca, T., et al., A quasi-optical and corrugated waveguide microwave transmission system for simultaneous dynamic nuclear polarization NMR on two separate 14.1 T...
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02-22-2018 02:13 AM
Mechanisms of Protein Translocation on DNA Are DifferentiallyResponsive to Water Activity
Mechanisms of Protein Translocation on DNA Are DifferentiallyResponsive to Water Activity
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.6b00872/20161205/images/medium/bi-2016-008725_0004.gif
Biochemistry
DOI: 10.1021/acs.biochem.6b00872
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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12-06-2016 04:10 AM
[NMR paper] Non-covalent spin labeling of riboswitch RNAs to obtain long-range structural NMR restraints.
Non-covalent spin labeling of riboswitch RNAs to obtain long-range structural NMR restraints.
Related Articles Non-covalent spin labeling of riboswitch RNAs to obtain long-range structural NMR restraints.
ACS Chem Biol. 2014 Mar 27;
Authors: Helmling C, Bessi I, Wacker A, Schnorr KA, Jonker HR, Richter C, Wagner D, Kreibich M, Schwalbe H
Abstract
Paramagnetic relaxation enhancement (PRE) NMR is a powerful method to study structure, dynamics and function of proteins. Up to now, the application of PRE NMR on RNAs is a significant challenge...
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03-29-2014 01:00 PM
[NMR paper] Functional manipulation of a calcium binding protein from E. histolytica guided by paramagnetic NMR.
Functional manipulation of a calcium binding protein from E. histolytica guided by paramagnetic NMR.
Functional manipulation of a calcium binding protein from E. histolytica guided by paramagnetic NMR.
J Biol Chem. 2013 Jun 19;
Authors: Rout AK, Patel S, Gupta S, Shukla M, Saraswathi D, Bhattacharya A, Chary KV
Abstract
EhCaBP1, one of the calcium binding proteins from E. histolytica, is a two-domain EF-hand protein. The two domains of EhCaBP1 are structurally and functionally different from each other. However, both the domains are...
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06-21-2013 01:10 PM
[NMR paper] In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in
In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles In vivo 133Cs-NMR a probe for studying subcellular compartmentation and ion uptake in maize root tissue.
Biochim Biophys Acta. 1990 Sep 1;1054(2):169-75
Authors: Pfeffer PE, Rolin DB, Brauer D, Tu SI, Kumosinski TF
Three 133Cs-NMR signals were observed in the spectra of CsCl-perfused and CsCl-grown maize seedling root...
Optical manipulation of subcellular protein translocation using a photoactivatable covalent labeling system
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