NMR paper Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motion

		BioNMR > Educational resources > Journal club
[NMR paper] Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motion

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-24-2010, 07:36 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motion

Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motions.

Off-resonance rf fields in heteronuclear NMR: Application to the study of slow motions.

J Biomol NMR. 1997 Dec;10(4):363-72

Authors: Zinn-Justin S, Berthault P, Guenneugues M, Desvaux H

The advantages of using off-resonance rf fields in heteronuclear self-relaxation experiments are explored on a fully (15)N-enriched protein. It is firstly shown that in the absence of slow motions the longitudinal and transverse (15)N self-relaxation rate values derived with this method are in agreement with the ones measured by the classical inversion-recovery and Carr-Purcell-Meiboom-Gill (CPMG) sequences, respectively. Secondly, by comparing the (15)N transverse self-relaxation rates obtained by the proposed off-resonance sequence and by the CPMG sequence, 11 residues out of the 61 of toxin α are shown to exhibit a chemical exchange phenomenon in water on a time scale ranging from 1 µs to 100 ms. By varying the effective field amplitude, chemical exchange processes involving these residues are measured and the corresponding correlation times are evaluated without having assumed any motion model. Similar, though less precise, results are given by the analysis of the (15)N off-resonance self-relaxation rates on the basis of the Lipari-Szabo model to describe the fast internal dynamics of toxin α.

PMID: 20859782 [PubMed - in process]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Dynamic Nuclear Polarization at High Magnetic Fields in Liquids Publication year: 2011 Source: Progress in Nuclear Magnetic Resonance Spectroscopy, Available online 4 November 2011</br> C.Griesinger, M.Bennati, H.M.Vieth, C.Luchinat, G.*Parigi, ...</br> Highlights ? High field Dynamic Nuclear Polarization spectrometers for liquid samples have been constructed, working at 7, 9.2 and 14 T, respectively. ? The field dependence of the Overhauser DNP efficiency has been measured experimentally for the first time up to a field of 9.2 T and compared with experimental results from...	nmrlearner	Journal club	0	11-05-2011 09:21 AM
[NMR paper] Strategy for the study of paramagnetic proteins with slow electronic relaxation rates Strategy for the study of paramagnetic proteins with slow electronic relaxation rates by nmr spectroscopy: application to oxidized human ferredoxin. Related Articles Strategy for the study of paramagnetic proteins with slow electronic relaxation rates by nmr spectroscopy: application to oxidized human ferredoxin. J Am Chem Soc. 2004 May 5;126(17):5413-26 Authors: Machonkin TE, Westler WM, Markley JL NMR studies of paramagnetic proteins are hampered by the rapid relaxation of nuclei near the paramagnetic center, which prevents the application...	nmrlearner	Journal club	0	11-24-2010 09:51 PM
[NMR paper] Slow internal dynamics in proteins: application of NMR relaxation dispersion spectros Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme. Related Articles Slow internal dynamics in proteins: application of NMR relaxation dispersion spectroscopy to methyl groups in a cavity mutant of T4 lysozyme. J Am Chem Soc. 2002 Feb 20;124(7):1443-51 Authors: Mulder FA, Hon B, Mittermaier A, Dahlquist FW, Kay LE Recently developed carbon transverse relaxation dispersion experiments (Skrynnikov, N. R.; et al. J. Am. Chem. Soc. 2001, 123, 4556-4566) were...	nmrlearner	Journal club	0	11-24-2010 08:49 PM
[NMR paper] Analysis of slow interdomain motion of macromolecules using NMR relaxation data. Analysis of slow interdomain motion of macromolecules using NMR relaxation data. Related Articles Analysis of slow interdomain motion of macromolecules using NMR relaxation data. J Am Chem Soc. 2001 May 2;123(17):3953-9 Authors: Baber JL, Szabo A, Tjandra N The interpretation of NMR relaxation data for macromolecules possessing slow interdomain motions is considered. It is shown how the "extended model-free approach" can be used to analyze (15)N backbone relaxation data acquired at three different field strengths for Xenopus Ca(2+)-ligated...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[NMR paper] Protein NMR extends into new fields of structural biology. Protein NMR extends into new fields of structural biology. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein NMR extends into new fields of structural biology. Curr Opin Chem Biol. 1997 Oct;1(3):359-64 Authors: Cooke RM Advances in protein NMR have opened new doors for the understanding of macromolecular structure and interactions. Isotope-labelling approaches have extended the size limit for structure determination, the mapping of protein-ligand interactions is...	nmrlearner	Journal club	0	08-22-2010 05:08 PM
[NMR paper] Escherichia coli diacylglycerol kinase: a case study in the application of solution N Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein. Biophys J. 1997 Jun;72(6):2688-701 Authors: Vinogradova O,...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Escherichia coli diacylglycerol kinase: a case study in the application of solution N Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Escherichia coli diacylglycerol kinase: a case study in the application of solution NMR methods to an integral membrane protein. Biophys J. 1997 Jun;72(6):2688-701 Authors: Vinogradova O,...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] NMR experiments for the study of photointermediates: application to the photoactive y NMR experiments for the study of photointermediates: application to the photoactive yellow protein. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR experiments for the study of photointermediates: application to the photoactive yellow protein. J Magn Reson. 1999 Apr;137(2):443-7 Authors: Rubinstenn G, Vuister GW, Zwanenburg N, Hellingwerf KJ, Boelens R, Kaptein R	nmrlearner	Journal club	0	08-21-2010 04:03 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off