Related ArticlesNucleotide sequence and analysis of NMR, a negative-acting regulatory gene in the nitrogen circuit of Neurospora crassa.
Mol Gen Genet. 1990 Jun;222(1):120-8
Authors: Young JL, Jarai G, Fu YH, Marzluf GA
In Neurospora the expression of a set of unlinked structural genes, which allows utilization of various nitrogen-containing compounds, is controlled by the positive-acting nit-2 gene and the negative-acting nmr gene. The nucleotide sequence of the nmr gene has been determined and a long open reading frame which encodes a putative protein of 54854 daltons has been identified. A full-length cDNA clone was obtained and its the sequence revealed that the nmr gene contains no introns. The transcriptional start and stop sites have been mapped by S1 nuclease and primer extension. Site-directed mutagenesis was used to introduce stop codons at various locations in the nmr coding region. Transformation assays showed that the proteins lacking up to 16% of the carboxyl-terminus were still functional. Homology searches showed that the nmr protein is homologous to the yeast arginine regulatory gene AR-GRII.
[NMR paper] NMR study of nucleotide-induced changes in the nucleotide binding domain of Thermus t
NMR study of nucleotide-induced changes in the nucleotide binding domain of Thermus thermophilus Hsp70 chaperone DnaK: implications for the allosteric mechanism.
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J Biol Chem. 2004 Aug 6;279(32):33958-67
Authors: Revington M, Holder TM, Zuiderweg ER
We present an NMR investigation of the nucleotide-dependent conformational properties of a 44-kDa nucleotide binding...
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[NMR paper] Improved initial yields in C-terminal sequence analysis by thiohydantoin chemistry us
Improved initial yields in C-terminal sequence analysis by thiohydantoin chemistry using purified diphenylphosphoryl isothiocyanate: NMR evidence for a reaction intermediate in the coupling reaction.
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Anal Biochem. 2002 Aug 15;307(2):202-11
Authors: Graham K, Shively JE
A thiohydantoin method for C-terminal sequence analysis of...
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[NMR paper] The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits
The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein NIT2.
Related Articles The negative-acting NMR regulatory protein of Neurospora crassa binds to and inhibits the DNA-binding activity of the positive-acting nitrogen regulatory protein NIT2.
Biochemistry. 1995 Jul 11;34(27):8861-8
Authors: Xiao X, Fu YH, Marzluf GA
Structural genes of the nitrogen regulatory circuit of the filamentous fungus Neurospora crassa are under the control of...
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[NMR paper] Nucleotide binding and GTP hydrolysis by the 21-kDa product of the c-H-ras gene as mo
Nucleotide binding and GTP hydrolysis by the 21-kDa product of the c-H-ras gene as monitored by proton-NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Nucleotide binding and GTP hydrolysis by the 21-kDa product of the c-H-ras gene as monitored by proton-NMR spectroscopy.
Eur J Biochem. 1993 Apr 15;213(2):781-8
Authors: Löw A, Sprinzl M, Limmer S
Proton-NMR signals in the downfield region (below approximately 10...
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[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora
Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Related Articles Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Biochem Genet. 1991 Oct;29(9-10):447-59
Authors: Young JL, Marzluf GA
In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...
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[NMR paper] Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora
Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Related Articles Molecular comparison of the negative-acting nitrogen control gene, nmr, in Neurospora crassa and other Neurospora and fungal species.
Biochem Genet. 1991 Oct;29(9-10):447-59
Authors: Young JL, Marzluf GA
In Neurospora crassa, the expression of unlinked structural genes which encode nitrogen catabolic enzymes is subject to genetic and metabolic regulation. The negative-acting nmr regulatory gene...
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[NMR paper] Analysis of conventional and in vitro generated mutants of nmr, the negatively acting
Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa.
Related Articles Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa.
Mol Gen Genet. 1990 Jul;222(2-3):233-40
Authors: Jarai G, Marzluf GA
The nmr gene is the major negative regulatory gene in the nitrogen control circuit of Neurospora crassa, which, together with positive regulatory genes, governs the expression of multiple unlinked...