NMR paper A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra

		BioNMR > Educational resources > Journal club
[NMR paper] A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-17-2010, 11:15 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,698

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra

A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra and suppresses without destroying the water magnetization.

Related Articles A novel composite 90 degrees pulse sequence which provides distortionless NMR spectra and suppresses without destroying the water magnetization.

J Magn Reson. 1998 Jul;133(1):194-9

Authors: Sodano P, Landon C, Ptak M

A novel 90 degrees composite pulse sequence which allows one to record 1D and 2D NMR spectra without disturbing the water magnetization is described. A home-written program was used to optimize the pulse angles for which the pulse sequence response fitted best the desired excitation profile, producing a neat and distortionless spectrum with a broad null excitation at the carrier frequency. The resulting pulse sequence was first evaluated using the simulation program "PENCIL" and then tested on two protein samples. A 3.5 degrees phase shift of the last pulse was required to cancel correctly the water signal. The pulse scheme was appended to a NOESY pulse sequence. Inspection of the water cross section revealed interactions between water and some protons of drosomycine, a small insect antifungal protein.

PMID: 9654486 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] what is best pulse sequence for c13 edit noesy on vnmrj ? what is best pulse sequence for c13 edit noesy on vnmrj ? Hi What is best pulse sequence in bio pack on vnmrj for C-13 edit NOESY ? I am working with 12 kd size protein which can exist as homo dimers ( i.e total size of homo-dimer is 24 kd ). Most frequent problems we are encountering as follows !line broadening , baseline , phase correction problems ( huge signal at 2.8 ppm )in first increments and f2f3 planes huge strips errors ! Thanking you in advance !	nmrlearner	News from other NMR forums	0	01-10-2012 03:38 PM
[Question from NMRWiki Q&A forum] CT COSY pulse sequence programming CT COSY pulse sequence programming Hi, I want to make a CT-COSY pulse sequence. But I'm bother by incremented delay. When I type in the BaseLevelRelation.h this: IN=-INand add in the microprogram the delay before and after my pi pulse,it didn't work... It seems like it calculate the d28 delay from the IN28 increment, but doesn't want to apply this delay in the pulse sequence....Any idea ? Perhaps it's about some incrementation declaration or something like that ?	nmrlearner	News from other NMR forums	0	05-04-2011 05:33 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues! Micropost: Pulse Sequence Blues! Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity: Pulse Sequence Blues! NMR can also be fun :-) https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com	nmrlearner	News from NMR blogs	0	02-23-2011 11:21 PM
[NMR analysis blog] Micropost: Pulse Sequence Blues! Micropost: Pulse Sequence Blues! Now that ENC is practically around the corner, here is a link that shows how far this conference encourages the participants’ creativity: Pulse Sequence Blues! NMR can also be fun :-) https://blogger.googleusercontent.com/tracker/6953549091784501422-8328472542836523761?l=nmr-analysis.blogspot.com	nmrlearner	News from NMR blogs	0	02-23-2011 11:18 PM
[NMR paper] A sequential HNCA NMR pulse sequence for protein backbone assignment. A sequential HNCA NMR pulse sequence for protein backbone assignment. Related Articles A sequential HNCA NMR pulse sequence for protein backbone assignment. J Magn Reson. 2001 May;150(1):100-4 Authors: Meissner A, Sørensen OW The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter- and intraresidue correlations because the one-bond and two-bond J(NC(alpha)) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum exhibiting...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
[Question from NMRWiki Q&A forum] Best pulse sequence for HSQC in Guanidine-HCl Best pulse sequence for HSQC in Guanidine-HCl Hello, I would like to acquire a series of HSQC spectra of an 15N-labeled 12 kDa protein unfolding (over the course of hours) in 3M GuaHCl. I have access to a Varian 600 spectrometer with a conventional probe. Due to the high ionic strength of the sample, tuning and shimming is a little difficult. However, I can record a sort-of-decent HSQC using a WaterGate gradient enhanced WGgNhsqc sequence. I am limited to using a protein concentration of about 250 uM. I've determined the pw90 to be 12.125 at a tpwr of 63. Using WGgNhsqc, the protein...	nmrlearner	News from other NMR forums	0	10-23-2010 07:42 AM
[NMRwiki tweet] nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ? nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ? http://qa.nmrwiki.org/question/159/ nmrwiki: how to write #nmr pulse sequence with different carrier freqs in F1 and F3 ? http://qa.nmrwiki.org/question/159/ Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	08-22-2010 01:49 AM
[NMRwiki tweet] nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/w nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/wiki/index.php?title=HZQC/HDQC_for_Bruker_(hdqc_Zex.rp) nmrwiki: Download HZQC #NMR pulse sequence for Chemical Exchange http://nmrwiki.org/wiki/index.php?title=HZQC/HDQC_for_Bruker_(hdqc_Zex.rp) Source: NMRWiki tweets	nmrlearner	Twitter NMR	0	08-22-2010 01:49 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off