[NMR paper] Lipid-nanodiscs formed by paramagnetic metal chelated polymer for fast NMR data acquisition.
Lipid-nanodiscs formed by paramagnetic metal chelated polymer for fast NMR data acquisition.
Related Articles Lipid-nanodiscs formed by paramagnetic metal chelated polymer for fast NMR data acquisition.
Biochim Biophys Acta Biomembr. 2020 Apr 30;:183332
Authors: Di Mauro GM, Hardin NZ, Ramamoorthy A
Abstract
Lipid-nanodiscs have been shown to be an exciting innovation as a membrane-mimicking system for studies on membrane proteins by a variety of biophysical techniques, including NMR spectroscopy. Although NMR spectroscopy is...
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05-05-2020 04:08 AM
Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies
Joint non-uniform sampling of all incremented time delays for quicker acquisition in protein relaxation studies
Abstract
NMR relaxometry plays crucial role in studies of protein dynamics. The measurement of longitudinal and transverse relaxation rates of \(^{15}\) N is the main source of information on backbone motions. However, even the most basic approach exploiting a series of \(^{15}\) ...
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05-16-2017 06:53 AM
[NMR paper] Entire-Dataset Analysis of NMR Fast-Exchange Titration Spectra: A Mg(2+) Titration Analysis for HIV-1 Ribonuclease H Domain.
Entire-Dataset Analysis of NMR Fast-Exchange Titration Spectra: A Mg(2+) Titration Analysis for HIV-1 Ribonuclease H Domain.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Entire-Dataset Analysis of NMR Fast-Exchange Titration Spectra: A Mg(2+) Titration Analysis for HIV-1 Ribonuclease H Domain.
J Phys Chem B. 2016 Dec 15;120(49):12420-12431
Authors: Karki I, Christen MT, Spiriti J, Slack RL, Oda M, Kanaori K, Zuckerman DM, Ishima R
Abstract
This...
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12-17-2016 07:18 AM
[U. of Ottawa NMR Facility Blog] Non-uniform Sampling (NUS)
Non-uniform Sampling (NUS)
Collecting 2D or 3D NMR data can be very time consuming. The indirect dimension of a 2D experiment is sampled linearly via the t1 increments in the pulse sequence. An FID must be collected for every single linearly spaced t1 increment. In the interest in collecting 2D or 3D NMR data in a more time efficient manner, a great deal of effort is made towards faster data collection techniques. While some of these methods are based on spatial selectivity, others are based on sparse sampling techniques in the indirect dimensions of nD NMR sequences. One such sparse...
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05-11-2016 08:04 PM
[NMR paper] Non-uniform sampling of NMR relaxation data.
Non-uniform sampling of NMR relaxation data.
Related Articles Non-uniform sampling of NMR relaxation data.
J Biomol NMR. 2016 Feb 4;
Authors: Linnet TE, Teilum K
Abstract
The use of non-uniform sampling of NMR spectra may give significant reductions in the data acquisition time. For quantitative experiments such as the measurement of spin relaxation rates, non-uniform sampling is however not widely used as inaccuracies in peak intensities may lead to errors in the extracted dynamic parameters. By systematic reducing the coverage...
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02-06-2016 03:10 PM
Non-uniform sampling of NMR relaxation data
Non-uniform sampling of NMR relaxation data
Abstract
The use of non-uniform sampling of NMR spectra may give significant reductions in the data acquisition time. For quantitative experiments such as the measurement of spin relaxation rates, non-uniform sampling is however not widely used as inaccuracies in peak intensities may lead to errors in the extracted dynamic parameters. By systematic reducing the coverage of the Nyquist grid of 15N Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion datasets for four different proteins and performing a full...
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02-05-2016 02:38 PM
Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance.
Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance.
Fast NMR Data Acquisition From Bicelles Containing a Membrane-Associated Peptide at Natural-Abundance.
J Phys Chem B. 2011 Sep 22;
Authors: Yamamoto K, Vivekanandan S, Ramamoorthy A
Abstract
In spite of recent technological advances in NMR spectroscopy, its low sensitivity continues to be a major limitation particularly for the structural studies of membrane proteins. The need for a large quantity of a membrane protein and acquisition of...
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09-24-2011 04:11 PM
[Question from NMRWiki Q&A forum] How to process and analyze serial 1D data nmrPipe?
How to process and analyze serial 1D data nmrPipe?
Hi all,I am very new to nmrPipe. I want to achieve the following steps. It would be very kind if somebody helps.
I have a 2D bruker data (t2= 4k points, t1= 32 points) and I want to do Fourier transform in the F2 dimension,phase etc. (2) and then extract the 32 spectra as 1Ds and also convert these 32 spectra in ascii format.(3) fit the peaks with a gaussian or Lorentzian function.(4) integrate the peak and save the integrals and time points as an ascii file.
I only managed till the 1st step.Please help..!!
Non-stationary complementary non-uniform sampling (NOSCO NUS) for fast acquisition of serial 2D NMR titration data
Linear Mode
