In drug development programmes, multiple assays are needed for the determination of protein-compound interactions and evaluation of potential use in assays with protein-protein interactions. In this protocol we describe the waterLOGSY NMR method for confirming protein-ligand binding events.
[NMR paper] Domain-specific interactions between MLN8237 and human serum albumin estimated by STD and WaterLOGSY NMR, ITC, spectroscopic, and docking techniques.
Domain-specific interactions between MLN8237 and human serum albumin estimated by STD and WaterLOGSY NMR, ITC, spectroscopic, and docking techniques.
Related Articles Domain-specific interactions between MLN8237 and human serum albumin estimated by STD and WaterLOGSY NMR, ITC, spectroscopic, and docking techniques.
Sci Rep. 2017 Mar 30;7:45514
Authors: Yang H, Liu J, Huang Y, Gao R, Tang B, Li S, He J, Li H
Abstract
Alisertib (MLN8237) is an orally administered inhibitor of Aurora A kinase. This small-molecule inhibitor is under...
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03-31-2017 12:43 PM
[NMR paper] Investigations into the binding of jadomycin DS to human topoisomerase II? by WaterLOGSY NMR spectroscopy.
Investigations into the binding of jadomycin DS to human topoisomerase II? by WaterLOGSY NMR spectroscopy.
Related Articles Investigations into the binding of jadomycin DS to human topoisomerase II? by WaterLOGSY NMR spectroscopy.
Org Biomol Chem. 2015 Aug 26;
Authors: Martinez-Farina CF, McCormick N, Robertson AW, Clement H, Jee A, Ampaw A, Chan NL, Syvitski RT, Jakeman DL
Abstract
The jadomycins are a family of secondary metabolites produced by S. venezuelae ISP5230. Specific jadomycins have been shown to possess a variety of...
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08-27-2015 12:37 PM
[NMR paper] Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments.
J Biomol NMR. 2014 Sep;60(1):37-44
Authors: Antanasijevic A, Ramirez B, Caffrey M
Abstract
The WaterLOGSY (WL) and saturation transfer difference (STD) NMR experiments have proven...
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05-13-2015 02:01 PM
[Question from NMRWiki Q&A forum] WaterLOGSY pulse program for bruker
WaterLOGSY pulse program for bruker
Hello,
I was wondering if there was a waterLOGSY sequence among the bruker sequences. If not, could someone tell me where I could find one?
There's something called ePHOGSY but I don't think that's the right sequence.
Thanks,
nmrlearner
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03-11-2015 09:59 PM
waterLOGSY experiment
Is there any step by step description of how to set up waterlogsy experiment for people with no big experience?
Thanks.
zc902
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01-05-2015 07:22 AM
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments
Comparison of the sensitivities of WaterLOGSY and saturation transfer difference NMR experiments
Abstract
The WaterLOGSY (WL) and saturation transfer difference (STD) NMR experiments have proven to be extremely useful techniques to characterize interactions between small molecules and large biomolecules. In this work we compare the relative sensitivities of WL and STD NMR using 3 experimental systems: ketoprofen (KET)â??bovine serum albumin (BSA), tert-butyl hydroquinone (TBHQ)â??hemagglutinin (HA), and chloramphenicol (CAM)â??ribosome (70S). In all...
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07-12-2014 06:07 PM
[NMR paper] Use of (1)h-nmr std, waterlogsy and langmuir monolayer techniques for characterization of drug- zein protein complexes.
Use of (1)h-nmr std, waterlogsy and langmuir monolayer techniques for characterization of drug- zein protein complexes.
Related Articles Use of (1)h-nmr std, waterlogsy and langmuir monolayer techniques for characterization of drug- zein protein complexes.
Eur J Pharm Biopharm. 2013 Jul 24;
Authors: Sousa FF, Luzardo-Álvarez A, Blanco-Méndez J, Otero-Espinar FJ, Martín-Pastor M, Sandez Macho I
Abstract
Zein is a protein based natural biopolymer containing a large amount of nonpolar amino acids, which has shown the ability to form...
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07-31-2013 12:00 PM
Use of 1h-nmr std, waterlogsy and langmuir monolayer techniques for characterization of drug- zein protein complexes
Use of 1h-nmr std, waterlogsy and langmuir monolayer techniques for characterization of drug- zein protein complexes
Publication date: Available online 24 July 2013
Source:European Journal of Pharmaceutics and Biopharmaceutics</br>
Author(s): F.F.O. Sousa , A. Luzardo-Álvarez , J. Blanco-Méndez , F.J. Otero-Espinar , M. Martín-Pastor , I. Sandez Macho</br>
Zein is a protein based natural biopolymer containing a large amount of nonpolar amino acids, which has shown the ability to form aggregates and entrap solutes, such as drugs and amino acids to form stable...
NMR waterLOGSY as An Assay in Drug Development Programmes for Detecting Protein-Ligand Interactions-NMR waterLOGSY
Linear Mode
