NMR unveils an N-terminal interaction interface on acetylated-{alpha}-synuclein monomers for recruitment to fibrils [Biophysics and Computational Biology]

		BioNMR > Educational resources > Journal club
NMR unveils an N-terminal interaction interface on acetylated-{alpha}-synuclein monomers for recruitment to fibrils [Biophysics and Computational Biology]

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

04-27-2021, 10:40 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR unveils an N-terminal interaction interface on acetylated-{alpha}-synuclein monomers for recruitment to fibrils [Biophysics and Computational Biology]

NMR unveils an N-terminal interaction interface on acetylated-{alpha}-synuclein monomers for recruitment to fibrils [Biophysics and Computational Biology]

Xue Yang, Baifan Wang, Cody L. Hoop, Jonathan K. Williams, Jean Baum...
Date: 2021-04-26

Amyloid fibril formation of ?-synuclein (?S) is associated with multiple neurodegenerative diseases, including Parkinson’s disease (PD). Growing evidence suggests that progression of PD is linked to cell-to-cell propagation of ?S fibrils, which leads to seeding of endogenous intrinsically disordered monomer via templated elongation and secondary nucleation. A molecular understanding of... Read More

PNAS:
Number: 18
Volume: 118

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac [Biophysics and Computational Biology] Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac Monica N. Kinde, Vasyl Bondarenko, Daniele Granata, Weiming Bu, Kimberly C. Grasty, Patrick J. Loll, Vincenzo Carnevale, Michael L. Klein, Roderic G. Eckenhoff, Pei Tang, Yan Xu... Date: 2016-11-29 Voltage-gated sodium channels (NaV) play an important role in general anesthesia. Electrophysiology measurements suggest that volatile anesthetics such as isoflurane inhibit NaV by stabilizing the inactivated state or altering the inactivation kinetics. Recent computational...	nmrlearner	Journal club	0	11-29-2016 09:02 PM
HIV-1 leader RNA dimeric interface revealed by NMR [Biophysics and Computational Biology] HIV-1 leader RNA dimeric interface revealed by NMR Laura R. Ganser, Hashim M. Al-Hashimi... Date: 2016-11-22 The genomes of retroviruses, such as HIV, consist of two single-stranded RNAs held together as a dimer through noncovalent interactions near the 5?-end. Why retroviruses carry two copies of their genetic material when most other viruses carry a single copy is something of a mystery. The single-stranded genomic RNA (gRNA)... Read More PNAS: Number: 47	nmrlearner	Journal club	0	11-22-2016 08:26 PM
NMR detection of intermolecular interaction sites in the dimeric 5'-leader of the HIV-1 genome [Biophysics and Computational Biology] NMR detection of intermolecular interaction sites in the dimeric 5'-leader of the HIV-1 genome Sarah C. Keane, Verna Van, Heather M. Frank, Carly A. Sciandra, Sayo McCowin, Justin Santos, Xiao Heng, Michael F. Summers... Date: 2016-11-15 HIV type-1 (HIV-1) contains a pseudodiploid RNA genome that is selected for packaging and maintained in virions as a noncovalently linked dimer. Genome dimerization is mediated by conserved elements within the 5?-leader of the RNA, including a palindromic dimer initiation signal (DIS) that has been proposed to form kissing hairpin... Read More ...	nmrlearner	Journal club	0	11-19-2016 08:35 PM
Protein NMR structures at >=100-kHz MAS [Biophysics and Computational Biology] Protein NMR structures at >=100-kHz MAS Andreas, L. B., Jaudzems, K., Stanek, J., Lalli, D., Bertarello, A., Le Marchand, T., Cala-De Paepe, D., Kotelovica, S., Akopjana, I., Knott, B., Wegner, S., Engelke, F., Lesage, A., Emsley, L., Tars, K., Herrmann, T., Pintacuda, G.... Date: 2016-08-16 Protein structure determination by proton-detected magic-angle spinning (MAS) NMR has focused on highly deuterated samples, in which only a small number of protons are introduced and observation of signals from side chains is extremely limited. Here, we show in two fully protonated proteins that,...	nmrlearner	Journal club	0	08-16-2016 07:38 PM
Structure of CAP-Gly on microtubules by MAS NMR [Biophysics and Computational Biology] Structure of CAP-Gly on microtubules by MAS NMR Yan, S., Guo, C., Hou, G., Zhang, H., Lu, X., Williams, J. C., Polenova, T.... Date: 2015-11-24 Microtubules and their associated proteins perform a broad array of essential physiological functions, including mitosis, polarization and differentiation, cell migration, and vesicle and organelle transport. As such, they have been extensively studied at multiple levels of resolution (e.g., from structural biology to cell biology). Despite these efforts, there remain significant... Read More PNAS: Number: 47	nmrlearner	Journal club	0	11-24-2015 09:32 PM
DNP MAS NMR on channelrhodopsin-2 [Biophysics and Computational Biology] DNP MAS NMR on channelrhodopsin-2 Becker-Baldus, J., Bamann, C., Saxena, K., Gustmann, H., Brown, L. J., Brown, R. C. D., Reiter, C., Bamberg, E., Wachtveitl, J., Schwalbe, H., Glaubitz, C.... Date: 2015-08-11 Channelrhodopsin-2 from Chlamydomonas reinhardtii is a light-gated ion channel. Over recent years, this ion channel has attracted considerable interest because of its unparalleled role in optogenetic applications. However, despite considerable efforts, an understanding of how molecular events during the photocycle, including the retinal trans-cis isomerization and the...	nmrlearner	Journal club	0	08-12-2015 08:58 AM
Protein dynamics from X-ray and NMR [Biophysics and Computational Biology] Protein dynamics from X-ray and NMR Fenwick, R. B., van den Bedem, H., Fraser, J. S., Wright, P. E.... Date: 2014-01-28 Detailed descriptions of atomic coordinates and motions are required for an understanding of protein dynamics and their relation to molecular recognition, catalytic function, and allostery. Historically, NMR relaxation measurements have played a dominant role in the determination of the amplitudes and timescales (picosecond–nanosecond) of bond vector fluctuations, whereas high-resolution X-ray... Read More PNAS: Number: 4	nmrlearner	Journal club	0	01-29-2014 12:50 AM
[NMR paper] Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization. Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization. PLoS One. 2013;8(1):e53487 Authors: ...	nmrlearner	Journal club	0	02-03-2013 10:19 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off