Abstract
A simple method for rapid structure screening for RNA by NMR is proposed. Target RNA is transcribed in a NMR tube and its spectra are measured without purification. The proposed method, in NMR tube transcription or INTT, was applied for three RNAs for which NMR spectra have been measured by using the conventionally purified samples. By the real-time measuring, increase of imino proton signals and decrease of NTP signals can be observed. It was confirmed that INTT spectra are in general similar to those obtained by the conventional method. INTT can be used for the first-step screening of RNA folding.
Rapid NMR screening of RNA secondary structure and binding
Rapid NMR screening of RNA secondary structure and binding
Abstract
Determination of RNA secondary structures by NMR spectroscopy is a useful tool e.g. to elucidate RNA folding space or functional aspects of regulatory RNA elements. However, current approaches of RNA synthesis and preparation are usually time-consuming and do not provide analysis with single nucleotide precision when applied for a large number of different RNA sequences. Here, we significantly improve the yield and 3â?² end homogeneity of RNA preparation by in vitro transcription....
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07-19-2015 02:36 PM
Spin-Label CW Microwave Power Saturation and Rapid Passage with Triangular Non-Adiabatic Rapid Sweep (NARS) and Adiabatic Rapid Passage (ARP) EPR Spectroscopy
Spin-Label CW Microwave Power Saturation and Rapid Passage with Triangular Non-Adiabatic Rapid Sweep (NARS) and Adiabatic Rapid Passage (ARP) EPR Spectroscopy
Publication date: Available online 11 April 2015
Source:Journal of Magnetic Resonance</br>
Author(s): Aaron W. Kittell , James S. Hyde</br>
Non-adiabatic rapid passage (NARS) electron paramagnetic resonance (EPR) spectroscopy was introduced by Kittell, A.W., Camenisch, T.G., Ratke, J.J. Sidabras, J.W., Hyde, J.S., 2011 as a general purpose technique to collect the pure absorption response. The technique has...
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[NMR paper] Rapid screening and identification of ?-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR.
Rapid screening and identification of ?-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles Rapid screening and identification of ?-glucosidase inhibitors from mulberry leaves using enzyme-immobilized magnetic beads coupled with HPLC/MS and NMR.
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Authors: Tao Y, Zhang Y, Cheng Y, Wang Y
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Rapid acquisition of (1) H and (19) F NMR experiments for direct and competition ligand-based screening.
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Magn Reson Chem. 2011 Mar 9;
Authors: Dalvit C, Gossert AD, Coutant J, Piotto M
Direct and competition ligand-based NMR experiments are often used in the screening of chemical fragment libraries against a protein target due to the high relative sensitivity of NMR for protein-binding events. A plethora of NMR methods has been proposed...
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[NMR paper] A general NMR method for rapid, efficient, and reliable biochemical screening.
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High-throughput screening is usually the method of drug-lead discovery. It is now well accepted that, for a functional assay, quality is more important than quantity. The ligand-based or protein-based NMR screening methodologies for detecting compounds...
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Curr Protoc Protein Sci. 2003 May;Chapter 7:Unit 7.11
Authors: Gronenborn AM
Assessing whether a protein or protein complex is amenable to structural analysis is an important component in the structural genomics effort. In particular, if complete sets of structures for entire genomes are to be obtained within a reasonable time frame, high throughput methodologies for all steps along the way have to be developed....
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[NMR paper] Microdrop screening: a rapid method to optimize solvent conditions for NMR spectrosco
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Determining appropriate solvent conditions is a crucial first step for carrying out NMR spectroscopy of proteins, but rapid and efficient methods for doing so are currently lacking. Microdrop screening examines a large number of different solvent conditions using...
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11-17-2010 11:15 PM
Easy way to clean NMR tube
Easy way to clean NMR tube
This article demonstrate the easy way to clean NMR tubes. It's simple but quite useful for those working with NMR.
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In NMR tube transcription for rapid screening of RNA conformation.
Linear Mode
