Related ArticlesNMR studies of peptides derived from the putative binding regions of cartilage proteins. No evidence for binding to hyaluronan.
J Biol Chem. 1994 Jan 21;269(3):1699-704
Authors: Horita DA, Hajduk PJ, Goetinck PF, Lerner LE
Previous work has implicated sequences within the tandem repeats of cartilage link protein in the interaction of link protein with hyaluronan. This conclusion was based on competitive inhibition experiments using synthetic peptides (Goetinck, P. F., Stirpe, N. S., Tsonis, P. A., and Carlone, D. (1987) J. Cell Biol. 105, 2403-2407). Further investigation of this system using high resolution nuclear magnetic resonance, circular dichroism, and competitive inhibition with other peptides indicates that the previously observed inhibition of link protein-hyaluronan binding was not caused by peptide-hyaluronan interactions. Instead, nonspecific aggregation of the peptides with link protein is proposed to account for all of the experimental data. Consequently, there is no direct experimental evidence to support the conclusion that these sequences in the tandem repeats of link protein are responsible for the link protein-hyaluronan interaction. If these peptides do represent the hyaluronan binding regions of link protein, these results imply a highly structure-dependent interaction between link protein and hyaluronan. Conformational analysis of the peptides using two-dimensional nuclear Overhauser spectroscopy indicates that the linear peptides do not adopt any stable secondary structure. However, several residues in the disulfide-looped peptides exhibit connectivities, suggesting a relatively long-lived extended chain conformation, consistent with predictions of secondary structure based on sequence analysis.
[NMR paper] NMR structures of anti-HIV D-peptides derived from the N-terminus of viral chemokine vMIP-II.
NMR structures of anti-HIV D-peptides derived from the N-terminus of viral chemokine vMIP-II.
Related Articles NMR structures of anti-HIV D-peptides derived from the N-terminus of viral chemokine vMIP-II.
Biochem Biophys Res Commun. 2005 Sep 30;335(3):651-8
Authors: Mori M, Liu D, Kumar S, Huang Z
The viral macrophage inflammatory protein-II (vMIP-II) encoded by Kaposi's sarcoma-associated herpesvirus has unique biological activities in that it blocks the cell entry by several different human immunodeficiency virus type 1 (HIV-1) strains via...
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12-01-2010 06:56 PM
[NMR paper] Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protei
Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protein kinases. An isolated but fully functional Gbeta binding domain from Ste20p is only partially folded as shown by heteronuclear NMR spectroscopy.
Related Articles Molecular interactions of the Gbeta binding domain of the Ste20p/PAK family of protein kinases. An isolated but fully functional Gbeta binding domain from Ste20p is only partially folded as shown by heteronuclear NMR spectroscopy.
J Biol Chem. 2001 Nov 2;276(44):41205-12
Authors: Song J, Chen Z, Xu P, Gingras R,...
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11-19-2010 08:44 PM
[NMR paper] 600 MHz NMR studies of human articular cartilage keratan sulfates.
600 MHz NMR studies of human articular cartilage keratan sulfates.
Related Articles 600 MHz NMR studies of human articular cartilage keratan sulfates.
Eur J Biochem. 1999 Dec;266(3):1174-83
Authors: Huckerby TN, Nieduszynski IA, Bayliss MT, Brown GM
The use of high-field two-dimensional 1H-correlation data is described for the detailed comparison of intact keratan sulfate polymer chains derived from human articular cartilage sources as a function of age. For fetal material the nonreducing chain termini are shown to be sparsely capped by sialyl...
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11-18-2010 08:31 PM
[NMR paper] Helical and coiled-coil-forming properties of peptides derived from and inhibiting hu
Helical and coiled-coil-forming properties of peptides derived from and inhibiting human immunodeficiency virus type 1 integrase assessed by 1H-NMR--use of NH temperature coefficients to probe coiled-coil structures.
Related Articles Helical and coiled-coil-forming properties of peptides derived from and inhibiting human immunodeficiency virus type 1 integrase assessed by 1H-NMR--use of NH temperature coefficients to probe coiled-coil structures.
Eur J Biochem. 1998 Apr 1;253(1):236-44
Authors: Krebs D, Maroun RG, Sourgen F, Troalen F, Davoust D,...
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11-17-2010 11:06 PM
[NMR paper] NMR and circular dichroism studies of synthetic peptides derived from the third intra
NMR and circular dichroism studies of synthetic peptides derived from the third intracellular loop of the beta-adrenoceptor.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR and circular dichroism studies of synthetic peptides derived from the third intracellular loop of the beta-adrenoceptor.
FEBS Lett. 1995 Jan 23;358(2):133-6
Authors: Jung H, Windhaber R, Palm D, Schnackerz KD
The C-terminal part of the third intracellular loop of the beta-adrenoceptor is capable...
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08-22-2010 03:41 AM
[NMR paper] NMR observation of substrate in the binding site of an active sugar-H+ symport protei
NMR observation of substrate in the binding site of an active sugar-H+ symport protein in native membranes.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR observation of substrate in the binding site of an active sugar-H+ symport protein in native membranes.
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3877-81
Authors: Spooner PJ, Rutherford NG, Watts A, Henderson PJ
NMR methods have been adopted to observe directly the characteristics of substrate...
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08-22-2010 03:33 AM
[NMR paper] NMR studies of peptides derived from the putative binding regions of cartilage protei
NMR studies of peptides derived from the putative binding regions of cartilage proteins. No evidence for binding to hyaluronan.
Related Articles NMR studies of peptides derived from the putative binding regions of cartilage proteins. No evidence for binding to hyaluronan.
J Biol Chem. 1994 Jan 21;269(3):1699-704
Authors: Horita DA, Hajduk PJ, Goetinck PF, Lerner LE
Previous work has implicated sequences within the tandem repeats of cartilage link protein in the interaction of link protein with hyaluronan. This conclusion was based on...
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08-22-2010 03:33 AM
[NMR paper] Conformational preferences of synthetic peptides derived from the immunodominant site
Conformational preferences of synthetic peptides derived from the immunodominant site of the circumsporozoite protein of Plasmodium falciparum by 1H NMR.
Related Articles Conformational preferences of synthetic peptides derived from the immunodominant site of the circumsporozoite protein of Plasmodium falciparum by 1H NMR.
Biochemistry. 1990 Aug 28;29(34):7828-37
Authors: Dyson HJ, Satterthwait AC, Lerner RA, Wright PE
Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational...