Related ArticlesNMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
Growth Factors. 1994;11(4):271-6
Authors: Maurer T, Smith DK, Owczarek CM, Layton MJ, Zhang JG, Nicola NA, Norton RS
Leukaemia inhibitory factor (LIF) is a polyfunctional cytokine active on many cell types. We present here 1H NMR studies on the solution properties and stability of MH35, a chimera of murine and human LIF which can be expressed at high levels in Escherichia coli, thus enabling efficient labelling of the protein with the stable isotopes 13C and 15N. MH35 has 85% sequence identity with human LIF and similar activity in biological assays. The 1H chemical shifts of the 12 conserved aromatic residues and the pKa values of the five conserved histidine residues in MH35 and human LIF are very similar. Temperature dependence studies indicate that both proteins are stable, with significant conformational changes occurring only above 70 degrees C. These results show that these proteins have a similar overall structure and stability and that MH35 is therefore a suitable analogue of human LIF for structural studies in solution.
[NMR paper] Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine
Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine leukemia virus (MoMLV) capsid protein major-homology-region peptide analogs by NMR spectroscopy.
Related Articles Solution structures of human immunodeficiency virus type 1 (HIV-1) and moloney murine leukemia virus (MoMLV) capsid protein major-homology-region peptide analogs by NMR spectroscopy.
Eur J Biochem. 1998 Oct 1;257(1):69-77
Authors: Clish CB, Peyton DH, Barklis E
The capsid domain of retroviral Gag proteins possesses a single highly conserved...
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[NMR paper] NMR solution structure of the 32-kDa platelet factor 4 ELR-motif N-terminal chimera:
NMR solution structure of the 32-kDa platelet factor 4 ELR-motif N-terminal chimera: a symmetric tetramer.
NMR solution structure of the 32-kDa platelet factor 4 ELR-motif N-terminal chimera: a symmetric tetramer.
Biochemistry. 1995 Sep 12;34(36):11399-409
Authors: Mayo KH, Roongta V, Ilyina E, Milius R, Barker S, Quinlan C, La Rosa G, Daly TJ
Native human platelet factor 4 (PF4) is a homotetrameric protein (70 residues/subunit) known for its anticoagulant heparin binding activity. 2D 15N--1H HSQC NMR experiments of native PF4 in solution...
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[NMR paper] NMR studies of the solution properties of recombinant murine interleukin-6.
NMR studies of the solution properties of recombinant murine interleukin-6.
Related Articles NMR studies of the solution properties of recombinant murine interleukin-6.
Biochim Biophys Acta. 1995 Jun 12;1249(2):189-203
Authors: Morton CJ, Bai H, Zhang JG, Hammacher A, Norton RS, Simpson RJ, Mabbutt BC
The effects of solvent, pH and temperature on the 1H-NMR spectra of recombinant murine interleukin-6 (IL-6) are described. Assignments made from two-dimensional homonuclear spectra are presented for resonances of the fifteen aromatic amino-acid...
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[NMR paper] NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Compariso
NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
Related Articles NMR studies of a murine-human chimera of leukaemia inhibitory factor (LIF). Comparison with human LIF.
Growth Factors. 1994;11(4):271-6
Authors: Maurer T, Smith DK, Owczarek CM, Layton MJ, Zhang JG, Nicola NA, Norton RS
Leukaemia inhibitory factor (LIF) is a polyfunctional cytokine active on many cell types. We present here 1H NMR studies on the solution properties and stability of MH35, a chimera of murine and human LIF...
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08-22-2010 03:33 AM
[NMR paper] Comparative studies of the interaction of human and bovine platelet factor 4 with hep
Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes.
Related Articles Comparative studies of the interaction of human and bovine platelet factor 4 with heparin using histidine NMR resonances as spectroscopic probes.
J Protein Chem. 1993 Jun;12(3):303-9
Authors: Talpas CJ, Lee L
The pKa values of His-38 and His-50 of the heparin-binding protein, bovine platelet factor 4, are 5.6 and 6.5, respectively, as determined by 1H NMR spectroscopy. The 1H NMR...
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08-21-2010 11:53 PM
[NMR paper] 1H-NMR assignment and secondary structure of human insulin-like growth factor-I (IGF-
1H-NMR assignment and secondary structure of human insulin-like growth factor-I (IGF-I) in solution.
Related Articles 1H-NMR assignment and secondary structure of human insulin-like growth factor-I (IGF-I) in solution.
J Biochem. 1992 Apr;111(4):529-36
Authors: Sato A, Nishimura S, Ohkubo T, Kyogoku Y, Koyama S, Kobayashi M, Yasuda T, Kobayashi Y
Human insulin-like growth factor-I (IGF-I) was studied by two-dimensional 1H-NMR spectroscopy. Resonance assignments were obtained for all the backbone protons and almost all of the sidechain protons...
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[NMR paper] Solution structure of murine epidermal growth factor determined by NMR spectroscopy a
Solution structure of murine epidermal growth factor determined by NMR spectroscopy and refined by energy minimization with restraints.
Related Articles Solution structure of murine epidermal growth factor determined by NMR spectroscopy and refined by energy minimization with restraints.
Biochemistry. 1992 Jan 14;31(1):236-49
Authors: Montelione GT, Wüthrich K, Burgess AW, Nice EC, Wagner G, Gibson KD, Scheraga HA
The solution structure of murine epidermal growth factor (mEGF) at pH 3.1 and a temperature of 28 degrees C has been determined...