In the formulation of efficient drug delivery systems, it is essential to unravel the structural and dynamical aspects of the drug's interaction with biological membranes. This has been done for the anticancer drug-membrane system comprising doxorubicin hydrochloride (DOX), a water-soluble anticancer drug, and the micellar sodium dodecyl sulfate (SDS), the latter serving as a useful mimic for membrane proteins. Using a multimodal NMR approach involving ¹H, ²H, and ^(13)C as probe nuclei and...
[NMR paper] Biochemical and NMR studies reveal specific interaction between STIMATE C-tail and PI(4,5)P(2) or PI(3,4,5)P(3)-containing membrane
Biochemical and NMR studies reveal specific interaction between STIMATE C-tail and PI(4,5)P(2) or PI(3,4,5)P(3)-containing membrane
STIMATE is an endoplasmic reticulum (ER) resident membrane protein that plays key roles in regulating calcium signaling occurring at ER-plasma membrane (PM) junctions. It is also involved in the regulation of ER-PM junction maintenance. STIMATE contains multiple putative transmembrane domains with a polybasic C tail (STIMATE-CT) that directly interacts with stromal interaction molecule 1 (STIM1) to promote STIM1 conformational switch. Here using liposome...
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02-07-2022 02:46 PM
Membrane Protein Structure in Live Cells: Methodology for Studying Drug Interaction by Mass Spectrometry-Based Footprinting
Membrane Protein Structure in Live Cells: Methodology for Studying Drug Interaction by Mass Spectrometry-Based Footprinting
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00874/20171220/images/medium/bi-2017-00874m_0009.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00874
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/m9uAQk5ldxQ
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12-20-2017 10:12 PM
An AdaptablePhospholipid Membrane Mimetic Systemfor Solution NMR Studies of Membrane Proteins
An AdaptablePhospholipid Membrane Mimetic Systemfor Solution NMR Studies of Membrane Proteins
Chih-Ta Henry Chien, Lukas R. Helfinger, Mark J. Bostock, Andras Solt, Yi Lei Tan and Daniel Nietlispach
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/jacs.7b06730/20171010/images/medium/ja-2017-067305_0005.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.7b06730
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/tJ3_oI8Z-O8
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10-11-2017 10:37 AM
[NMR paper] An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.
An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.
An adaptable phospholipid membrane mimetic system for solution NMR studies of membrane proteins.
J Am Chem Soc. 2017 Oct 09;:
Authors: Chien CH, Helfinger LR, Bostock MJ, Solt A, Tan YL, Nietlispach D
Abstract
Based on the saposin-A (SapA) scaffold protein we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins under close-to-native conditions. The Salipro...
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10-11-2017 10:37 AM
[NMR paper] NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
Related Articles NMR studies of the interaction between inner membrane-associated and periplasmic cytochromes from Geobacter sulfurreducens.
FEBS Lett. 2017 May 24;:
Authors: Dantas JM, Brausemann A, Einsle O, Salgueiro CA
Abstract
Geobacter sulfurreducens is a dissimilatory metal reducing bacterium with notable properties and significance in biotechnological applications. Biochemical studies suggest that...
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05-26-2017 08:36 PM
Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments.
Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments.
Comparative NMR analysis of an 80-residue G protein-coupled receptor fragment in two membrane mimetic environments.
Biochim Biophys Acta. 2011 Jul 23;
Authors: Cohen LS, Arshava B, Neumoin A, Becker JM, Güntert P, Zerbe O, Naider F
Fragments of integral membrane proteins have been used to study the physical chemical properties of regions of transporters and receptors. Ste2p(G31-T110) is an 80-residue polypeptide which contains a...
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07-28-2011 10:51 AM
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Transferred NOESY NMR studies of biotin mimetic peptide (FSHPQNT) bound to streptavidin: A structural model for studies of peptide-protein interactions.
Chem Biol Drug Des. 2011 Feb 5;
Authors: Gizachew D, Dratz E
Protein-protein interactions control signaling, specific adhesion and many other biological functions. The three dimensional structures of the interfaces and bound ligand can be...
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02-08-2011 06:28 PM
[NMR paper] NMR studies of the antibody-bound conformation of a carbohydrate-mimetic peptide.
NMR studies of the antibody-bound conformation of a carbohydrate-mimetic peptide.
Related Articles NMR studies of the antibody-bound conformation of a carbohydrate-mimetic peptide.
Biochemistry. 2002 Feb 19;41(7):2149-57
Authors: Johnson MA, Rotondo A, Pinto BM
Transferred nuclear Overhauser enhancement (TRNOE) experiments have been performed at 800 MHz to investigate the bound conformation of the hexapeptide DRPVPY, a functional molecular mimic of the group A Streptococcus cell-wall polysaccharide. The hexapeptide binds to the monoclonal...
NMR Studies on the Interaction of Anticancer Drug Doxorubicin with Membrane Mimetic SDS
Linear Mode
