NMR paper NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-rece

		BioNMR > Educational resources > Journal club
[NMR paper] NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-rece

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-24-2010, 08:49 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,791

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-rece

NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction.

Related Articles NMR studies of CCK-8/CCK1 complex support membrane-associated pathway for ligand-receptor interaction.

Can J Physiol Pharmacol. 2002 May;80(5):383-7

Authors: Giragossian C, Pellegrini M, Mierke DF

The interaction of peptide ligands with their associated G-protein-coupled receptors has been examined by a number of different experimental approaches over the years. We have been developing an approach utilizing high-resolution NMR to determine the structural features of the peptide ligand, well-designed fragments of the receptor, and the ligand-receptor complexes formed upon titration of the peptide hormone. The results from these investigations provide evidence for a membrane-associated pathway for the initial interaction of peptide ligands with the receptor. Here, our results from the investigation of the interaction of CCK-8 with the CCK1 receptor are described. Our spectroscopic results clearly show that both CCK-8 and the regions of CCK1 with which it interacts are closely associated with the zwitterionic interface of the lipids utilized in our solution spectroscopic studies.

PMID: 12056543 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction. Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction. Portrayal of complex dynamic properties of sugarcane defensin 5 by NMR: multiple motions associated with membrane interaction. Structure. 2011 Jan 12;19(1):26-36 Authors: de Paula VS, Razzera G, Barreto-Bergter E, Almeida FC, Valente AP Defensins are essentially ancient natural antibiotics with potent activity extending from lower organisms to humans. Sd5 is a recently described antifungal defensin that appears to be the...	nmrlearner	Journal club	0	04-19-2011 11:01 PM
Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB. Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB. Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB. J Am Chem Soc. 2011 Mar 4; Authors: Tang M, Sperling LJ, Berthold DA, Nesbitt AE, Gennis RB, Rienstra CM Ubiquinone (Coenzyme Q) plays an important role in the mitochondrial respiratory chain and also acts as an antioxidant in its reduced form, protecting cellular membranes from peroxidation....	nmrlearner	Journal club	0	03-08-2011 01:40 PM
Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB Solid-State NMR Study of the Charge-Transfer Complex between Ubiquinone-8 and Disulfide Bond Generating Membrane Protein DsbB Ming Tang, Lindsay J. Sperling, Deborah A. Berthold, Anna E. Nesbitt, Robert B. Gennis and Chad M. Rienstra http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja107775w/aop/images/medium/ja-2010-07775w_0004.gif Journal of the American Chemical Society DOI: 10.1021/ja107775w http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/WdFsSgH1V7w	nmrlearner	Journal club	0	03-05-2011 02:44 AM
[NMR paper] Bead-linked proteoliposomes: a reconstitution method for nmr analyses of membrane protein-ligand interactions. Bead-linked proteoliposomes: a reconstitution method for nmr analyses of membrane protein-ligand interactions. Related Articles Bead-linked proteoliposomes: a reconstitution method for nmr analyses of membrane protein-ligand interactions. J Am Chem Soc. 2005 Aug 31;127(34):12021-7 Authors: Yokogawa M, Takeuchi K, Shimada I Structural information about the interactions between membrane proteins and their ligands provides insights into the membrane protein functions. A variety of surfactants have been used for structural analyses of membrane...	nmrlearner	Journal club	0	12-01-2010 06:56 PM
[NMR paper] NMR analysis of the transient complex between membrane photosystem I and soluble cyto NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6. Related Articles NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6. J Biol Chem. 2005 Mar 4;280(9):7925-31 Authors: Díaz-Moreno I, Díaz-Quintana A, Molina-Heredia FP, Nieto PM, Hansson O, De la Rosa MA, Karlsson BG A structural analysis of the surface areas of cytochrome c(6), responsible for the transient interaction with photosystem I, was performed by NMR transverse relaxation-optimized spectroscopy....	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] Studies of protein-ligand interactions by NMR. Studies of protein-ligand interactions by NMR. Related Articles Studies of protein-ligand interactions by NMR. Biochem Soc Trans. 2003 Oct;31(Pt 5):1006-9 Authors: Clarkson J, Campbell ID Solution-state NMR has become an accepted method for studying the structure of small proteins in solution. This has resulted in over 3000 NMR-based co-ordinate sets being deposited in the Protein Databank. It is becoming increasingly apparent, however, that NMR is also a very powerful tool for accessing interactions between macromolecules and various ligands....	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] Backbone NMR assignments of a high molecular weight protein (47 kDa), cyclic AMP rece Backbone NMR assignments of a high molecular weight protein (47 kDa), cyclic AMP receptor protein (apo-CRP) Related Articles Backbone NMR assignments of a high molecular weight protein (47 kDa), cyclic AMP receptor protein (apo-CRP) J Biomol NMR. 2000 Jan;16(1):79-80 Authors: Won HS, Yamazaki T, Lee TW, Jee JG, Yoon MK, Park SH, Otomo T, Aiba H, Kyogoku Y, Lee BJ	nmrlearner	Journal club	0	11-18-2010 09:15 PM
Ligand-membrane protein binding by solid-state NMR Selective Interface Detection: Mapping Binding Site Contacts in Membrane Proteins by NMR Spectroscopy Suzanne R. Kiihne, Alain F. L. Creemers, Willem J. de Grip, Petra H. M. Bovee-Geurts, Johan Lugtenburg, and Huub J. M. de Groot J. Am. Chem. Soc.; 2005; 127(16) pp 5734 - 5735 ABSTRACT: Intermolecular contact surfaces are important regions where specific interactions mediate biological function. We introduce a new magic angle spinning solid state NMR technique, dubbed "selective interface detection spectroscopy" (SIDY). In this technique, 13C-attached protons (1Hlig) are dephased by...	nmrlearner	Journal club	0	05-19-2005 09:04 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off