Related ArticlesNMR studies of Azotobacter vinelandii and Pseudomonas putida seven-iron ferredoxins. Direct assignment of beta-cysteinyl carbon NMR resonances and further proton NMR assignments of cysteinyl and aromatic resonances.
J Biol Chem. 1992 Apr 25;267(12):8073-80
Authors: Cheng H, Grohmann K, Sweeney W
Ferredoxins are proteins which contain iron and inorganic sulfide and are capable of electron transport. They are found in a wide range of organisms, from anaerobic bacteria, to plants and mammals. Although NMR spectroscopy has been used to study ferredoxins since the 1970s, little important structural or biochemical information has resulted from these investigations. The major difficulty has been the effect of the paramagnetic iron-sulfur clusters on the peptide resonances, hindering nuclear Overhauser effect (NOE) studies and causing broad line widths. These effects are most pronounced on resonances arising from the nuclei closest to the iron-sulfur center. Unfortunately, these are likely to be the most interesting nuclei, as they report the events and geometry in the vicinity of the active sites. In this paper, the first direct assignment of beta-cysteinyl 13C resonances for any iron-sulfur protein is reported for the spectrum of Pseudomonas putida ferredoxin. These resonances are of special significance, as they arise from the atoms on the protein closest to the iron centers, with the exception of the directly bound cysteinyl sulfur atoms. In addition, cysteinyl and ring system 1H NMR resonance assignments are made for the spectra of P. putida ferredoxin and Azotobacter vinelandii ferredoxin I.
[NMR paper] Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by
Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by NMR spectroscopy and solution X-ray scattering and interactions with rubredoxin reductase.
Related Articles Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by NMR spectroscopy and solution X-ray scattering and interactions with rubredoxin reductase.
Biochemistry. 2004 Mar 23;43(11):3167-82
Authors: Perry A, Tambyrajah W, Grossmann JG, Lian LY, Scrutton NS
Here we provide insights into the molecular structure of the two-iron...
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[NMR paper] Structural rearrangements of the two domains of Azotobacter vinelandii rhodanese upon
Structural rearrangements of the two domains of Azotobacter vinelandii rhodanese upon sulfane sulfur release: essential molecular dynamics, 15N NMR relaxation and deuterium exchange on the uniformly labeled protein.
Related Articles Structural rearrangements of the two domains of Azotobacter vinelandii rhodanese upon sulfane sulfur release: essential molecular dynamics, 15N NMR relaxation and deuterium exchange on the uniformly labeled protein.
Int J Biol Macromol. 2003 Dec;33(4-5):193-201
Authors: Cicero DO, Melino S, Orsale M, Brancato G, Amadei A,...
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[NMR paper] Two-iron rubredoxin of Pseudomonas oleovorans: production, stability and characteriza
Two-iron rubredoxin of Pseudomonas oleovorans: production, stability and characterization of the individual iron-binding domains by optical, CD and NMR spectroscopies.
Related Articles Two-iron rubredoxin of Pseudomonas oleovorans: production, stability and characterization of the individual iron-binding domains by optical, CD and NMR spectroscopies.
Biochem J. 2001 Feb 15;354(Pt 1):89-98
Authors: Perry A, Lian LY, Scrutton NS
A minigene encoding the C-terminal domain of the 2Fe rubredoxin of Pseudomonas oleovorans was created from the...
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[NMR paper] Iron uptake by ferritin: NMR relaxometry studies at low iron loads.
Iron uptake by ferritin: NMR relaxometry studies at low iron loads.
Related Articles Iron uptake by ferritin: NMR relaxometry studies at low iron loads.
J Inorg Biochem. 1998 Sep;71(3-4):153-7
Authors: Vymazal J, Brooks RA, Bulte JW, Gordon D, Aisen P
Twenty ferritin samples were prepared at pH 6.5 with average loadings of 0-89 Fe atoms per molecule. Nuclear magnetic relaxation times T1 and T2 were measured at 3 degrees C, 23 degrees C, and at 37 degrees C and at field strength from 0.025 to 1.5 T. The field dependence, temperature dependence,...
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[NMR paper] Recombinant two-iron rubredoxin of Pseudomonas oleovorans: overexpression, purificati
Recombinant two-iron rubredoxin of Pseudomonas oleovorans: overexpression, purification and characterization by optical, CD and 113Cd NMR spectroscopies.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.biochemj.org-images-bj_pubmed.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Recombinant two-iron rubredoxin of Pseudomonas oleovorans: overexpression, purification and characterization by optical, CD and 113Cd NMR spectroscopies.
Biochem J. 1997 Nov 15;328 ( Pt...
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[NMR paper] In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
Related Articles In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
J Biol Chem. 1991 Jun 25;266(18):11705-13
Authors: Jones JG, Bellion E
Pseudomonas species MA was grown with methylamine as a sole source of carbon and nitrogen enabling the total flow of carbon and nitrogen into this organism to be simultaneously monitored in vivo using 13C and 15N NMR. Methylamine was rapidly and extensively incorporated into the methyl...
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[NMR paper] NMR studies on p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens and salicyl
NMR studies on p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens and salicylate hydroxylase from Pseudomonas putida.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles NMR studies on p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens and salicylate hydroxylase from Pseudomonas putida.
Eur J Biochem. 1991 Sep 15;200(3):731-8
Authors: Vervoort J, Van Berkel WJ, Müller F, Moonen CT
p-Hydroxybenzoate hydroxylase from...
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[NMR paper] The downfield resonances in the 1H NMR spectra of Azotobacter vinelandii and Pseudomo
The downfield resonances in the 1H NMR spectra of Azotobacter vinelandii and Pseudomonas putida seven-iron ferredoxins.
Related Articles The downfield resonances in the 1H NMR spectra of Azotobacter vinelandii and Pseudomonas putida seven-iron ferredoxins.
J Biol Chem. 1990 Jul 25;265(21):12388-92
Authors: Cheng H, Grohmann K, Sweeney W
Pseudomonas putida and Azotobacter vinelandii ferredoxins each contain one cluster and one cluster. Their polypeptide chains are nearly identical, differing by only 15 residues out of a total of 106. T1...