Related ArticlesNMR studies on the 46-kDa dimeric protein, 3,4-dihydroxy-2-butanone 4-phosphate synthase, using 2H, 13C, and 15N-labelling.
Eur J Biochem. 1999 Apr;261(1):57-65
Authors: Richter G, Kelly M, Krieger C, Yu Y, Bermel W, Karlsson G, Bacher A, Oschkinat H
3,4-Dihydroxy-2-butanone 4-phosphate synthase catalyses the release of C-4 from the substrate, ribulose phosphate, via a complex series of rearrangement reactions. The cognate ribB gene of Escherichia coli was hyperexpressed in a recombinant E. coli strain. The protein was shown to be a 46-kDa homodimer by hydrodynamic analysis. A variety of protein samples labelled with different grades of 13C, 15N and 2H, i.e. one with 100% 2H and 15N, one with 75% 2H, 99% 13C, 15N, and one with 100% 2H, 99% 13C,15N were prepared. Despite the large molecular size, 2- and 3-dimensional NMR spectra of reasonable quality were obtained. Attempts at the assignment of individual 13C, 15N and 1H signals show, in principle, the feasibility of structure determination. The number of NMR signals shows unequivocally that the homodimeric protein obeys strict C2 symmetry.
NMR Studies of the Stability, Protonation States, and Tautomerism of 13C- and 15N-Labeled Aldimines of the Coenzyme Pyridoxal 5?-Phosphate in Water
NMR Studies of the Stability, Protonation States, and Tautomerism of 13C- and 15N-Labeled Aldimines of the Coenzyme Pyridoxal 5?-Phosphate in Water
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/bi101061m/aop/images/medium/bi-2010-01061m_0010.gif
Biochemistry
DOI: 10.1021/bi101061m
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/bichaw/~4/YIwxDgj3pEA
More...
nmrlearner
Journal club
0
12-07-2010 09:54 AM
[NMR paper] Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dy
Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Related Articles Protein stabilization by compatible solutes. Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR.
Eur J Biochem. 2003 Dec;270(23):4606-14
Authors: Lamosa P, Turner DL, Ventura R, Maycock C, Santos H
Heteronuclear NMR relaxation measurements and hydrogen exchange data have been used to characterize protein dynamics in the presence or absence of...
nmrlearner
Journal club
0
11-24-2010 09:16 PM
[NMR paper] Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P
Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization.
Related Articles Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization.
Calcif Tissue Int. 2003 May;72(5):610-26
Authors: Wu Y, Ackerman JL, Strawich ES, Rey C, Kim HM, Glimcher MJ
Previous 31P cross-polarization and differential cross-polarization magic angle spinning (CP/MAS and DCP/MAS) solid-state NMR...
nmrlearner
Journal club
0
11-24-2010 09:01 PM
[NMR paper] The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate s
The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site.
Related Articles The NMR structure of the 47-kDa dimeric enzyme 3,4-dihydroxy-2-butanone-4-phosphate synthase and ligand binding studies reveal the location of the active site.
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13025-30
Authors: Kelly MJ, Ball LJ, Krieger C, Yu Y, Fischer M, Schiffmann S, Schmieder P, Kühne R, Bermel W, Bacher A, Richter G, Oschkinat H
Recent developments in...
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[NMR paper] NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyri
NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyribose conformational exchange in DNA.
Related Articles NMR evidence for mechanical coupling of phosphate B(I)-B(II) transitions with deoxyribose conformational exchange in DNA.
J Mol Biol. 2001 Aug 3;311(1):149-60
Authors: Isaacs RJ, Spielmann HP
The conformational exchange of the phosphate and deoxyribose groups of the DNA oligomers d(GCGTACGC)(2) and d(CGCTAGCG)(2) have been investigated using a combination of homonuclear and heteronuclear NMR techniques....
nmrlearner
Journal club
0
11-19-2010 08:44 PM
[NMR paper] A compact monomeric intermediate identified by NMR in the denaturation of dimeric tri
A compact monomeric intermediate identified by NMR in the denaturation of dimeric triose phosphate isomerase.
Related Articles A compact monomeric intermediate identified by NMR in the denaturation of dimeric triose phosphate isomerase.
J Mol Biol. 2000 Jun 30;300(1):11-6
Authors: Morgan CJ, Wilkins DK, Smith LJ, Kawata Y, Dobson CM
The denaturation of triose phosphate isomerase (TIM) from Saccharomyces cerevisiae by guanidine hydrochlorids at pH 7.2 has been monitored by NMR spectroscopy in conjunction with optical spectroscopy. In the...
nmrlearner
Journal club
0
11-18-2010 09:15 PM
[NMR paper] 31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotro
31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles 31P NMR studies of phosphate metabolism in the ovulatory process induced by gonadotropins in perfused rabbit ovary.
NMR Biomed. 1999 Jun;12(4):249-55
Authors: Tanaka K, Mizukami A, Kojima N, Ishikawa M
The concentrations of phosphate metabolites were measured in perfused...