Related ArticlesNMR structures of ?-proteobacterial ATPase-regulating ?-subunits.
J Mol Biol. 2014 May 13;
Authors: Serrano P, Geralt M, Mohanty B, Wüthrich K
Abstract
NMR structures of ?-subunits, which are recently discovered ?-proteobacterial F1F0-ATPase regulatory proteins representing a Pfam protein family of 246 sequences from 219 species (PF07345), exhibit a four-helix bundle, which is different from all other known F1F0-ATPase inhibitors. Chemical shift mapping reveals a conserved ADP/ATP-binding site in ?-subunit, which mediates long-range conformational changes related to function, as revealed by the structure of the Paracoccus denitrificans ?-subunit in complex with ADP. These structural data suggest a new mechanism of F1F0-ATPase regulation in ?-proteobacteria.
PMID: 24838125 [PubMed - as supplied by publisher]
[NMR paper] LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.
LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-2250-98-WileyOnlineLibrary-Button_120x27px_FullText.gif Related Articles LEGO-NMR Spectroscopy: A Method to Visualize Individual Subunits in Large Heteromeric Complexes.
Angew Chem Int Ed Engl. 2013 Aug 14;
Authors: Mund M, Overbeck JH, Ullmann J, Sprangers R
Abstract
Seeing the big picture: Asymmetric macromolecular complexes that are NMR active in...
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[NMR paper] Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae.
Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-standard-jbc_final.gif Related Articles Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae.
J Biol Chem. 2013 Apr 26;288(17):11930-9
Authors: Basak S, Lim J, Manimekalai MS, Balakrishna AM, Grüber G
...
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Modular protein-RNA interactions regulating mRNA metabolism: a role for NMR.
Modular protein-RNA interactions regulating mRNA metabolism: a role for NMR.
Modular protein-RNA interactions regulating mRNA metabolism: a role for NMR.
Eur Biophys J. 2011 Apr 7;
Authors: Cukier CD, Ramos A
Here we review the role played by transient interactions between multi-functional proteins and their RNA targets in the regulation of mRNA metabolism, and we describe the important function of NMR spectroscopy in the study of these systems. We place emphasis on a general approach for the study of different features of modular multi-domain...
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[NMR paper] Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy.
Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy.
Related Articles Structural characterization of the interaction of the delta and alpha subunits of the Escherichia coli F1F0-ATP synthase by NMR spectroscopy.
Biochemistry. 2005 Sep 6;44(35):11786-94
Authors: Wilkens S, Borchardt D, Weber J, Senior AE
A critical point of interaction between F(1) and F(0) in the bacterial F(1)F(0)-ATP synthase is formed by the alpha and delta subunits. Previous work has...
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[NMR paper] Overexpression, purification, and characterization of recombinant Ca-ATPase regulator
Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
Related Articles Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
Protein Expr Purif. 2003 Aug;30(2):253-61
Authors: Buck B, Zamoon J, Kirby TL, DeSilva TM, Karim C, Thomas D, Veglia G
Phospholamban (PLB) and Sarcolipin (SLN) are integral membrane proteins that regulate muscle contractility via direct...
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[NMR paper] Conformational analysis of a mitochondrial presequence derived from the F1-ATPase bet
Conformational analysis of a mitochondrial presequence derived from the F1-ATPase beta-subunit by CD and NMR spectroscopy.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Conformational analysis of a mitochondrial presequence derived from the F1-ATPase beta-subunit by CD and NMR spectroscopy.
Biochim Biophys Acta. 1992 Sep 4;1159(1):81-93
Authors: Bruch MD, Hoyt DW
Previous studies on mitochondrial targeting presequences have indicated that formation of an amphiphillic...
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[NMR paper] 19F and 31P NMR spectroscopy of G protein alpha subunits. Mechanism of activation by
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J Biol Chem. 1991 Feb 25;266(6):3396-401
Authors: Higashijima T, Graziano MP, Suga H, Kainosho M, Gilman AG
19F and 31P NMR spectroscopy was used to study the mechanism of activation of the alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) by Al3+, Mg2+, and F-. 19F NMR spectra of solutions containing Al3+,...
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[NMR paper] An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Related Articles An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Kurume Med J. 1990;37(3):153-7
Authors: Chinami M, Shingu M
The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial...
NMR structures of ?-proteobacterial ATPase-regulating ?-subunits.
Linear Mode
