Related ArticlesNMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
J Mol Biol. 1997 Mar 7;266(4):649-55
Authors: Jelinek R, Terry TD, Gesell JJ, Malik P, Perham RN, Opella SJ
An NMR approach for structure determination of short peptides displayed on the surface of filamentous bacteriophage virions is demonstrated using the hexapeptide GPGRAF that constitutes the principal neutralizing determinant of HIV-1. This peptide was inserted near the N terminus of the major coat protein of bacteriophage fd. NMR studies of the recombinant protein solubilized in detergent micelles showed that the inserted peptide adopts a double bend S-shaped conformation that is similar to the antibody-bound structure determined by X-ray crystallography. This indicates that a peptide displayed on the bacteriophage coat protein has an enhanced propensity to adopt a conformation similar to that found in the native protein from which it is derived. This approach may be generally applicable to the structure determination of peptide epitopes and other small peptides.
Principal Scientist - Protein Crystallography - Pfizer - Groton, CT, United States
Principal Scientist - Protein Crystallography - Pfizer - Groton, CT, United States
Please apply on-line at http://www.pfizer.com/careers and search for job ID 953226.
Role Description
The successful candidate for this position will join Groton Structural Biology and Biophysics (SB&B), a multi-disciplinary group with well-established expertise in molecular biology, protein biochemistry, biological mass spectrometry, NMR,...
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08-03-2011 01:00 AM
Senior Principal Scientist | Roche
Senior Principal Scientist | Roche
US - Nutley, NJ, USA, - Minimum of a PhD in Analytical Chemistry (or equivalent in related experience) with track history of managing teams- Recognized strong scientific background in analytical chemistry and NMR and/or
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07-20-2011 10:24 PM
[NMR paper] NMR structural analysis of alpha-bungarotoxin and its complex with the principal alph
NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor.
Related Articles NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor.
J Biol Chem. 2002 Apr 5;277(14):12406-17
Authors: Moise L, Piserchio A, Basus VJ, Hawrot E
We report a new, higher resolution NMR structure of...
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11-24-2010 08:49 PM
[NMR paper] Principal components analysis of protein structure ensembles calculated using NMR dat
Principal components analysis of protein structure ensembles calculated using NMR data.
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J Biomol NMR. 2001 May;20(1):61-70
Authors: Howe PW
One important problem when calculating structures of biomolecules from NMR data is distinguishing converged structures from outlier structures. This paper describes how Principal Components Analysis (PCA) has the potential to classify calculated structures automatically, according to correlated...
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11-19-2010 08:32 PM
[NMR paper] Structure of a malaria parasite antigenic determinant displayed on filamentous bacter
Structure of a malaria parasite antigenic determinant displayed on filamentous bacteriophage determined by NMR spectroscopy: implications for the structure of continuous peptide epitopes of proteins.
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Protein Sci. 2001 Jun;10(6):1150-9
Authors: Monette M, Opella SJ, Greenwood J, Willis AE, Perham RN
The NANP repeating sequence of...
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Principal Geoscientist NMR at Weatherford International (Houston, TX)
Principal Geoscientist NMR at Weatherford International (Houston, TX)
RESPONSIBILITIES: * Starting with a sound understanding of NMR theory, tool measurements and principles and combined ... * Experience in numerical modeling and/or experimental measurements of NMR tools * Excellent numeric and analytical...
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09-12-2010 02:01 AM
[NMR paper] NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamen
NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR structure of the principal neutralizing determinant of HIV-1 displayed in filamentous bacteriophage coat protein.
J Mol Biol. 1997 Mar 7;266(4):649-55
Authors: Jelinek R, Terry TD, Gesell JJ, Malik P, Perham RN, Opella SJ
An NMR approach for structure determination of short peptides displayed on the...
nmrlearner
Journal club
0
08-22-2010 03:31 PM
[NMR paper] The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of
The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR.
Biochemistry. 1996 Aug 13;35(32):10256-61
Authors: Grzesiek S, Stahl SJ, Wingfield PT, Bax A
Using heteronuclear NMR spectroscopy, we demonstrate that a 13-residue peptide (MS-QIKRLLSEKKT) from the...