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NMR processing:
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MARS
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Side-chains:
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NOEs:
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UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
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Fragment-based:
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GeNMR
I-TASSER
Refinement:
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Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
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CS23D
Simshift
Torsion angles from chemical shifts:
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TALOS
Promega- Proline
Secondary structure from chemical shifts:
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MICS caps, β-turns
d2D
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Flexibility from chemical shifts:
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Interactions from chemical shifts:
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Vasco
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SAVES2 or SAVES4
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MetaMQAPII
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Methyl S2
B-factor
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From structure:
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Sparta+
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CH3shift- Methyl
ArShift- Aromatic
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Proshift
PPM
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From sequence:
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Disordered proteins:
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From NMR-STAR 3.1
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NMR sample preparation:
Protein disorder:
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Protein solubility:
camLILA
ccSOL
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camGroEL
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Isotope labeling:
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Solid-state NMR:
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Old 09-10-2011, 06:51 PM
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Default NMR structure of an acyl-carrier protein from Borrelia burgdorferi.

NMR structure of an acyl-carrier protein from Borrelia burgdorferi.

NMR structure of an acyl-carrier protein from Borrelia burgdorferi.

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt 9):1137-40

Authors: Barnwal RP, Van Voorhis WC, Varani G

Abstract
Nearly complete resonance assignment and the high-resolution NMR structure of the acyl-carrier protein from Borrelia burgdorferi, a target of the Seattle Structural Genomics Center for Infectious Disease (SSGCID) structure-determination pipeline, are reported. This protein was chosen as a potential target for drug-discovery efforts because of its involvement in fatty-acid biosynthesis, an essential metabolic pathway, in bacteria. It was possible to assign >98% of backbone resonances and >92% of side-chain resonances using multidimensional NMR spectroscopy. The NMR structure was determined to a backbone r.m.s.d. of 0.4 Å and contained four ?-helices and two 3(10)-helices. A structure-homology search revealed that this protein is highly similar to the acyl-carrier protein from Aquifex aeolicus.


PMID: 21904063 [PubMed - in process]



Source: PubMed
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