Abstract
Sulfated fucans, sulfated galactans and glycosaminoglycans are extensively studied worldwide in terms of both structure and biomedical functions. Liquid-state nuclear magnetic resonance (NMR) spectroscopy is the most employed analytical technique in structural analysis of these sulfated glycans. This is due to the fact that NMR-based analyses enable a series of achievements such as (i) accurate structure characterization/determination; (ii) measurements of parameters regarding molecular motion (dynamics); (iii) assessment of the 3D structures (usually assisted by computational techniques of Molecular Modeling and/or Molecular Dynamics) of the composing monosaccharides (ring conformers) and the overall conformational states of the glycan chains either free in solution or bound to proteins; and (iv) analysis of the resultant intermolecular complexes with functional proteins through either the protein or the carbohydrate perspective. In this review, after a general introduction about the principal NMR parameters utilized for achieving this set of structural information, discussion is given on NMR-based studies of some representative sulfated fucans, sulfated galactans and glycosaminoglycans. Due to the growing number of studies concerning both structure and function of sulfated glycans in the field and the widely use of NMR spectroscopy in such studies, a review paper discussing (i) the most experiments employed for analysis, (ii) procedures used in data interpretation, and (iii) the general aspects of the sulfated glycans, is timely in the literature.
PMID: 27166778 [PubMed - as supplied by publisher]
[NMR paper] Structural Analysis of N- and O-glycans Using ZIC-HILIC/DIALYSIS Coupled to NMR Detection.
Structural Analysis of N- and O-glycans Using ZIC-HILIC/DIALYSIS Coupled to NMR Detection.
Related Articles Structural Analysis of N- and O-glycans Using ZIC-HILIC/DIALYSIS Coupled to NMR Detection.
Fungal Genet Biol. 2014 Aug 9;
Authors: Qu Y, Feng J, Deng S, Cao L, Zhang Q, Zhao R, Zhang Z, Jiang Y, Zink EM, Baker SE, Lipton MS, Paša-Toli? L, Hu JZ, Wu S
Abstract
Protein glycosylation, an important and complex post-translational modification (PTM), is involved in various biological processes, including the receptor-ligand and...
nmrlearner
Journal club
0
08-15-2014 12:53 PM
Postoctoral position in Structural Biology
Postoctoral position in Structural Biology
UNIVERSITY OF OXFORD
DEPARTMENT OF BIOCHEMISTRY
Postdoctoral Research Associate in Structural Biology
We are seeking to appoint a highly motivated Postdoctoral Research
Associate in the laboratory of Dr. Ioannis Vakonakis for studies of
proteins involved in centriole assembly. The successful candidate will
nmrlearner
Job marketplace
0
01-27-2012 04:34 PM
[Question from NMRWiki Q&A forum] 13C NMR of sulfated galactose
13C NMR of sulfated galactose
Hi everybody, Has anyone ever seen a C-6 at 64 ppm in the 13C NMR spectrum of a sulfated galactose?
Check if somebody has answered this question on NMRWiki QA forum
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States
Research Scientist Position in the Cryo-Electron Microscopy Facility at the New York Structural Biol - New York Structural Biology Center - New York, NY, United States
The New York Structural Biology Center (NYSBC) seeks an experienced electron microscopist to join the staff of its Cryo-Electron Microscope Facility (http://cryoem.nysbc.org). The NYSBC is shared center that supports state-of-the-art research in cryo-EM, NMR, and X-ray. Cryo-EM facilities include four transmission electron microscopes and a new d...
More...
nmrlearner
Job marketplace
0
01-26-2011 04:21 AM
[NMR paper] An account of NMR in structural biology.
An account of NMR in structural biology.
Related Articles An account of NMR in structural biology.
Nat Struct Biol. 1997 Oct;4 Suppl:841-4
Authors: Wagner G
With the ability to determine atomic resolution structures of biological macromolecules in semi-physiological conditions, nuclear magnetic resonance spectroscopy (NMR) has become an eminent tool in structural biology. NMR provides a means for studying critical biological phenomena including protein structure, dynamics and folding as well as a practical approach to drug design.
nmrlearner
Journal club
0
08-22-2010 05:08 PM
[NMR paper] Structure determination by 1H NMR spectroscopy of (sulfated) sialylated N-linked carb
Structure determination by 1H NMR spectroscopy of (sulfated) sialylated N-linked carbohydrate chains released from porcine thyroglobulin by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase-F.
Related Articles Structure determination by 1H NMR spectroscopy of (sulfated) sialylated N-linked carbohydrate chains released from porcine thyroglobulin by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase-F.
J Biol Chem. 1991 Mar 5;266(7):4237-43
Authors: de Waard P, Koorevaar A, Kamerling JP, Vliegenthart JF
The N-linked carbohydrate...
nmrlearner
Journal club
0
08-21-2010 11:16 PM
Prospects for lanthanides in structural biology by NMR
Prospects for lanthanides in structural biology by NMR
Gottfried Otting
Journal of Biomolecular NMR; 2008; 42(1); pp 1-9
Abstract:
The advent of different lanthanide-binding reagents has made site-specific labelling of proteins with paramagnetic lanthanides a viable proposition. This brings many powerful techniques originally established and demonstrated for paramagnetic metalloproteins into the mainstream of structural biology. The promise is that, by exploiting the long-range effects of paramagnetism, lanthanide labelling will allow the study of larger proteins and protein–ligand...
NMR Structural Biology of Sulfated Glycans.
Linear Mode
