Related ArticlesNMR spectroscopy and protein structure determination: applications to drug discovery and development.
Curr Pharm Biotechnol. 2005 Apr;6(2):105-20
Authors: Wishart D
Recent technological advances in NMR methods and instrumentation are having a significant impact in structural biology. These innovations are also impacting pharmaceutical biotechnology as it is now possible to use NMR spectroscopy to rapidly characterize a growing number of prospective protein drugs and protein drug targets. This review provides a general summary of how solution-state NMR can be used to determine protein structures. It also focuses on exploring how advances in solution state NMR are changing the way in which protein structures can be determined and protein-ligand interactions can be characterized. Recent innovations in protein sample preparation, in instrumentation and data collection, in spectral assignment and in structure generation are highlighted. The impact of solution-state NMR on pharmaceutical biotechnology is also discussed, with a special emphasis on describing how NMR has been used to study a number of pharmaceutically important proteins and how NMR is currently being used to rapidly screen and to map the binding sites of small molecules to a range of protein targets.
Researchers Look at Averting Drug Resistance - Drug Discovery & Development
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Researchers Look at Averting Drug Resistance
Drug Discovery & Development
Kern and her team studied the protein using nuclear magnetic resonance spectroscopy, adding a drug mimetic in order to learn how the structure EmrE was designed and how it functioned as it was transportingâ??moving the drug from the inside of the cell ...
Researchers Look at Averting Drug Resistance - Drug Discovery & Development
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02-02-2012 09:43 PM
Application of NMR and Molecular Docking in Structure-Based Drug Discovery.
Application of NMR and Molecular Docking in Structure-Based Drug Discovery.
Application of NMR and Molecular Docking in Structure-Based Drug Discovery.
Top Curr Chem. 2011 Sep 14;
Authors: Stark JL, Powers R
Abstract
Drug discovery is a complex and costly endeavor, where few drugs that reach the clinical testing phase make it to market. High-throughput screening (HTS) is the primary method used by the pharmaceutical industry to identify initial lead compounds. Unfortunately, HTS has a high failure rate and is not particularly efficient at...
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09-15-2011 08:31 PM
[NMR paper] NMR in the acceleration of drug discovery.
NMR in the acceleration of drug discovery.
Related Articles NMR in the acceleration of drug discovery.
Curr Opin Drug Discov Devel. 2001 Jul;4(4):479-92
Authors: Sem DS, Pellecchia M
The field of NMR spectroscopy has grown beyond expectations from the first historic observation of nuclear magnetic resonance in 1946 by Bloch and Purcell, to the first generation of protein structures 20 years ago, to the present where NMR structures now represent 15 to 20% of those submitted to the Protein Data Bank (PDB). However, the applications of NMR go far...
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11-19-2010 08:44 PM
[NMR paper] NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural produc
NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural products, and metabolism research.
Related Articles NMR spectroscopy in drug discovery: tools for combinatorial chemistry, natural products, and metabolism research.
Prog Drug Res. 2000;55:137-211
Authors: Keifer PA
NMR spectroscopy has enjoyed many advances recently, and the pace of development shows no signs of slowing. This article focuses on advances that have affected solution-state NMR. These advances fall into three general categories: new experimental...
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11-18-2010 09:15 PM
[NMR paper] Applications of NMR in drug discovery.
Applications of NMR in drug discovery.
Related Articles Applications of NMR in drug discovery.
Drug Discov Today. 2000 Jun;5(6):230-240
Authors: Roberts GC
In the half-century since its discovery, nuclear magnetic resonance (NMR) has become the single most powerful form of spectroscopy in both chemistry and structural biology. The dramatic technical advances over the past 10-15 years, which continue apace, have markedly increased the range of applications for NMR in the study of protein-ligand interactions. These form the basis for its most...
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11-18-2010 09:15 PM
[NMR paper] Flow NMR spectroscopy in drug discovery.
Flow NMR spectroscopy in drug discovery.
Related Articles Flow NMR spectroscopy in drug discovery.
Curr Opin Drug Discov Devel. 2000 May;3(3):269-74
Authors: Stockman BJ
Flow NMR spectroscopy techniques are becoming increasingly utilized in drug discovery and development. LC-NMR has become a routine method to resolve and identify mixture components. It has broad applications in natural products biochemistry, and drug metabolism and toxicology studies. The rapid throughput of direct-injection NMR of biofluids, combinatorial chemistry samples...
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11-18-2010 09:15 PM
Application of NMR Spectroscopy in Medicinal Chemistry and Drug Discovery.
Application of NMR Spectroscopy in Medicinal Chemistry and Drug Discovery.
Related Articles Application of NMR Spectroscopy in Medicinal Chemistry and Drug Discovery.
Curr Top Med Chem. 2010 Sep 2;
Authors: Ohno A, Inomata K, Tochio H, Shirakawa M
"In-cell nuclear magnetic resonance (NMR)" is a unique method for characterization of conformation, interaction and dynamics of proteins inside living cells at atomic level. Since the method was proposed by Dötch and co-workers in 2001 , its application had been limited to bacterial cells and...
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09-03-2010 02:30 PM
[NMR paper] NMR screening in drug discovery.
NMR screening in drug discovery.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR screening in drug discovery.
Curr Opin Biotechnol. 1999 Feb;10(1):54-8
Authors: Moore JM
NMR methods in drug discovery have traditionally been used to obtain structural information for drug targets or target-ligand complexes. Recently, it has been shown that NMR may be used as an alternative approach for identification of ligands that bind to protein drug targets, shifting the emphasis...
NMR spectroscopy and protein structure determination: applications to drug discovery
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