[NMR paper] NMR Spectroscopy and Electron Microscopy Identification of Metabolic and Ultrastructural Changes to the Kidney Following Ischemia Reperfusion Injury.
NMR Spectroscopy and Electron Microscopy Identification of Metabolic and Ultrastructural Changes to the Kidney Following Ischemia Reperfusion Injury.
Am J Physiol Renal Physiol. 2017 Oct 04;:ajprenal.00363.2017
Authors: Chihanga T, Ma Q, Nicholson JD, Ruby HN, Edelmann RE, Devarajan P, Kennedy MA
Abstract
Cellular, molecular, and ultrastructural nephron changes associated with ischemia reperfusion injury-induced acute kidney injury (IRI-AKI) are not completely understood. Here, a multidisciplinary study was used to identify nephron changes in a mouse model of IRI-AKI. Histological analyses indicated distended Bowman's glomerular spaces and proximal and distal tubules. Increased filtrate volume in nephrons was caused by reduced water reabsorption by severely damaged proximal tubule brush borders and blocked flow of filtrate into collecting tubules by mucoprotein casts in distal tubules. Immunohistochemistry revealed protein AKI biomarkers in proximal tubules and glomeruli but not in distal tubules. Nuclear magnetic resonance spectroscopy revealed several metabolites that increased such as valine alanine and lactate. Other metabolites such as trigonelline, succinate, 2-oxoisocaproate, and 1- methyl-nicotinamide decreased or were absent in urine following IRI due to altered kidney function or metabolism. Urinary glucose increased due to reduced reabsorption by damaged proximal tubule brush borders. Scanning electron microscopy revealed flattening of podocytes and pedicals surrounding glomerular capillaries and transmission electron microscopy (TEM) revealed effacement of podocyte pedicals, both consistent with increased hydrostatic pressure in nephrons following IRI-AKI. TEM revealed shortened proximal tubule microvilli in IRI kidneys with diminished lamina propia. TEM showed dramatic loss of mitochondria in distal tubule epithelia of IRI kidneys and emergence of multivesicular bodies of endosomes indicating ongoing cellular death. Collectively, the data define ultrastructural changes to nephrons and altered kidney metabolism associated with IRI-AKI.
PMID: 28978534 [PubMed - as supplied by publisher]
Cryo-electron microscopy: Alzheimer's fibrils
Cryo-electron microscopy: Alzheimer's fibrils
http://www.spectroscopynow.com/common/images/thumbnails/15ec800ad6f.jpgA technique that can augment X-ray crystallographic and spectroscopic studies has been demonstrated to provide an atomic resolution in imaging of the amyloid fibrils present in Alzheimer's disease, thanks to European research.
Read the rest at Spectroscopynow.com
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10-01-2017 06:06 AM
Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model
From The DNP-NMR Blog:
Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model
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Baligand, C., et al., Hyperpolarized 13 C magnetic resonance evaluation of renal ischemia reperfusion injury in a murine model. NMR Biomed, 2017. 30(10): p. e3765-n/a.
https://www.ncbi.nlm.nih.gov/pubmed/28708304
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09-27-2017 04:26 PM
[NMR paper] Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Salecan protected against concanavalin A-induced acute liver injury by modulating T cell immune responses and NMR-based metabolic profiles.
Toxicol Appl Pharmacol. 2017 Feb 15;317:63-72
Authors: Sun Q, Xu X, Yang X, Weng D, Wang J, Zhang J
Abstract
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06-01-2017 03:02 PM
Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury
From The DNP-NMR Blog:
Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury
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Nielsen, P.M., et al., Fumarase activity: an in vivo and in vitro biomarker for acute kidney injury. Scientific Reports, 2017. 7: p. 40812.
http://dx.doi.org/10.1038/srep40812
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05-09-2017 12:37 AM
[NMR paper] Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy.
Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full_free.gif Related Articles Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy.
Proc Natl Acad Sci U S A. 2015 Oct 13;
Authors: Sborgi L, Ravotti F, Dandey VP, Dick MS, Mazur A, Reckel S, Chami M, Scherer S, Huber M, Böckmann A, Egelman EH,...
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10-16-2015 01:44 PM
[NMR images] Other methods are Cryo-EM(electron microscopy),
http://3.bp.blogspot.com/_H3VvAQred7I/SSRk1W97JBI/AAAAAAAAAII/G32vZntGdTE/s400/NMR.jpg
7/06/2014 7:13:57 PM GMT
Other methods are Cryo-EM(electron microscopy),
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NMR Spectroscopy and Electron Microscopy Identification of Metabolic and Ultrastructural Changes to the Kidney Following Ischemia Reperfusion Injury.
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