This review highlights recent advancements in using high resolution nuclear magnetic resonance (NMR) spectroscopy as a characterization tool to expedite biologics formulation development, meeting a current need in the biopharmaceutical industry. Conformational changes of protein therapeutics during formulation development can result in various protein-protein and protein-excipient interactions, which can lead to physical aggregation and/or chemical degradation. Innovative analytical techniques...
[ASAP] Epitope Mapping and Binding Assessment by Solid-State NMR Provide a Way for the Development of Biologics under the Quality by Design Paradigm
Epitope Mapping and Binding Assessment by Solid-State NMR Provide a Way for the Development of Biologics under the Quality by Design Paradigm
Domenico Rizzo, Linda Cerofolini, Stefano Giuntini, Luisa Iozzino, Carlo Pergola, Francesca Sacco, Angelo Palmese, Enrico Ravera, Claudio Luchinat, Fabio Baroni, and Marco Fragai
https://pubs.acs.org/cms/10.1021/jacs.2c03232/asset/images/medium/ja2c03232_0010.gif
Journal of the American Chemical Society
DOI: 10.1021/jacs.2c03232
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05-27-2022 07:04 AM
[NMR paper] Epitope Mapping and Binding Assessment by Solid-State NMR Provide a Way for the Development of Biologics under the Quality by Design Paradigm
Epitope Mapping and Binding Assessment by Solid-State NMR Provide a Way for the Development of Biologics under the Quality by Design Paradigm
Multispecific biologics are an emerging class of drugs, in which antibodies and/or proteins designed to bind pharmacological targets are covalently linked or expressed as fusion proteins to increase both therapeutic efficacy and safety. Epitope mapping on the target proteins provides key information to improve the affinity and also to monitor the manufacturing process and drug stability. Solid-state NMR has been here used to identify the pattern of...
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05-27-2022 07:04 AM
[NMR paper] Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy
Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy
Protein therapeutics carry inherent limitations of membrane impermeability and structural instability, despite their predominant role in the modern pharmaceutical market. Effective formulations are needed to overcome physiological and physicochemical barriers, respectively, for improving bioavailability and stability. Knowledge of membrane affinity, cellular internalization, encapsulation, and release of drug-loaded carrier vehicles uncover the structural basis for designing and optimizing...
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02-24-2021 05:50 AM
[NMR paper] Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy.
Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy.
Related Articles Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy.
Adv Drug Deliv Rev. 2021 Feb 17;:
Authors: Phyo P, Zhao X, Templeton AC, Xu W, Cheung JK, Su Y
Abstract
Protein therapeutics carry inherent limitations of membrane impermeability and structural instability, despite their predominant role in the modern pharmaceutical market. Effective formulations are needed to overcome...
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02-21-2021 01:14 PM
NMR: an essential structural tool for integrative studies of T cell development, pMHC ligand recognition and TCR mechanobiology
NMR: an essential structural tool for integrative studies of T cell development, pMHC ligand recognition and TCR mechanobiology
Abstract
Early studies of T cell structural biology using X-ray crystallography, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) focused on a picture of the αβT cell receptor (αβTCR) component domains and their cognate ligands (peptides bound to MHC molecules, i.e. pMHCs) as static interaction partners. Moving forward requires integrating this corpus of data with dynamic technologies such as...
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03-24-2019 10:41 PM
[NMR paper] Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
J Pharm Biomed Anal. 2017 Feb 20;135:126-132
Authors: Bednarek E, Sitkowski J, Bocian W, Borowicz P, P?ucienniczak G, Stadnik D, Surmacz-Chwedoruk W,...
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05-26-2017 08:36 PM
Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy
From The DNP-NMR Blog:
Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy
Smith, A.N. and J.R. Long, Dynamic Nuclear Polarization as an Enabling Technology for Solid State Nuclear Magnetic Resonance Spectroscopy. Analytical Chemistry, 2016. 88(1): p. 122-132.
http://dx.doi.org/10.1021/acs.analchem.5b04376
NMR spectroscopy as a characterization tool enabling biologics formulation development
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