NMR paper NMR spectroscopy-based metabolomics of Drosophila model of Huntington's disease suggests altered cell energetics.

		BioNMR > Educational resources > Journal club
[NMR paper] NMR spectroscopy-based metabolomics of Drosophila model of Huntington's disease suggests altered cell energetics.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

09-06-2017, 04:13 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR spectroscopy-based metabolomics of Drosophila model of Huntington's disease suggests altered cell energetics.

NMR spectroscopy-based metabolomics of Drosophila model of Huntington's disease suggests altered cell energetics.

Related Articles NMR spectroscopy-based metabolomics of Drosophila model of Huntington's disease suggests altered cell energetics.

J Proteome Res. 2017 Sep 05;:

Authors: Singh V, Sharma RK, Athilingam T, Sinha P, Sinha N, Thakur AK

Abstract
Huntington's disease (HD) is a neurodegenerative disorder induced by aggregation of the pathological form of Huntingtin protein that has expanded polyglutamine (polyQ) repeats. In the Drosophila model, for instance, expression of transgenes with polyQ repeats induce HD-like pathologies progressively correlating with the increasing lengths of these repeats. Previous studies on both animal models and clinical samples have revealed metabolite imbalances during HD progression. To further explore the physiological processes linked to metabolite imbalances during HD, we have investigated 1D (1)H NMR spectroscopy-based metabolomics profile of Drosophila HD model. Using multivariate analysis (PCA and PLS-DA) of metabolites obtained from methanolic extracts of fly heads displaying retinal deformations due to polyQ overexpression, we show that the metabolite imbalance during HD are likely to affect cell energetics. Six out of the 35 metabolites analyzed, namely, nicotinamide adenine dinucleotide (NAD), lactate, pyruvate, succinate, sarcosine, and acetoin displayed segregation with progressive severity of HD. Specifically, HD progression was seen associated with reduction in NAD and increase in lactate-to-pyruvate ratio. Further, comparative analysis of fly HD metabolome with those of mouse HD model, and HD human patients, revealed comparable metabolite imbalances suggesting altered cellular energy homeostasis. These findings thus raise the possibility of therapeutic interventions for HD via modulation of cellular energetics.

PMID: 28871787 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Scientists Observe Structure of Protein That Plays Major Role in Huntington's Disease, Opening Door to Finding Cause ... - Huntington's Disease News Scientists Observe Structure of Protein That Plays Major Role in Huntington's Disease, Opening Door to Finding Cause ... - Huntington's Disease News http://www.bionmr.com//t0.gstatic.com/images?q=tbn:ANd9GcTwPt0kkc7URsZZYlq-KqB_9CkWqZ-RqmOnDrrW5h7hGiXSmY3KB8xsPpoKBTVsqkUarvvUCVGj Huntington's Disease News <img alt="" height="1" width="1"> Scientists Observe Structure of Protein That Plays Major Role in Huntington's Disease, Opening Door to Finding Cause ... Huntington's Disease News Leibniz-Institut für Molekulare Pharmakologie (FMP) researchers used a combination of nuclear magnetic...	nmrlearner	Online News	0	02-10-2016 09:28 PM
Protein Plaques in Huntington's Patients Have Distinct Structures That Hint at Disease Mechanisms - Huntington's Disease News <img alt="" height="1" width="1"> Protein Plaques in Huntington's Patients Have Distinct Structures That Hint at Disease Mechanisms Huntington's Disease News Researchers at the University of Pittsburgh School of Medicine used advanced nuclear magnetic resonance spectroscopy to directly assess and characterize huntingtin fibrils and other polyglutamine aggregates. Results showed that, unlike what previous ... Protein Plaques in Huntington's Patients Have Distinct Structures That Hint at Disease Mechanisms - Huntington's Disease News More...	nmrlearner	Online News	0	02-06-2016 03:05 AM
Study finding could shed light on molecular mechanisms underlying Huntington's disease - News-Medical.net http://www.bionmr.com//t3.gstatic.com/images?q=tbn:ANd9GcQ4bPSiC2GxHh2tSrBv-yw0PP4aV7KUqSJA__VTst6Y-abi8OKMJ34YPc5XvnrcWZZ7f9dPIKE Science World Report <img alt="" height="1" width="1"> Study finding could shed light on molecular mechanisms underlying Huntington's disease News-Medical.net ... the process," he said. "Using advanced nuclear magnetic resonance spectroscopy, we were able to provide an unprecedented view of the internal structure of the protein clumps that form in the disease, which we hope will one day lead to new therapies ... Researchers Describe Brain Plaques Involved In...	nmrlearner	Online News	0	02-04-2016 11:46 AM
[NMR paper] In vivo high-resolution magic angle spinning proton NMR spectroscopy of Drosophila melanogaster flies as a model system to investigate mitochondrial dysfunction in Drosophila GST2 mutants. In vivo high-resolution magic angle spinning proton NMR spectroscopy of Drosophila melanogaster flies as a model system to investigate mitochondrial dysfunction in Drosophila GST2 mutants. Related Articles In vivo high-resolution magic angle spinning proton NMR spectroscopy of Drosophila melanogaster flies as a model system to investigate mitochondrial dysfunction in Drosophila GST2 mutants. Int J Mol Med. 2014 Jul;34(1):327-33 Authors: Righi V, Apidianakis Y, Psychogios N, Rahme LG, Tompkins RG, Tzika AA Abstract In vivo nuclear...	nmrlearner	Journal club	0	12-31-2014 06:44 PM
[NMR paper] (1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease. (1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease. (1)H NMR-Based Metabolite Profiling of Plasma in a Rat Model of Chronic Kidney Disease. PLoS One. 2014 Jan 20;9(1):e85445 Authors: Kim JA, Choi HJ, Kwon YK, Ryu do H, Kwon TH, Hwang GS Abstract Chronic kidney disease (CKD) is characterized by the gradual loss of the kidney function to excrete wastes and fluids from the blood. (1)H NMR-based metabolomics was exploited to investigate the altered metabolic pattern in rats with CKD induced by surgical...	nmrlearner	Journal club	0	01-28-2014 11:53 AM
[NMR paper] NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers. NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers. Related Articles NMR-based metabolomics of urine in a mouse model of Alzheimer's disease: identification of oxidative stress biomarkers. J Clin Biochem Nutr. 2013 Mar;52(2):133-8 Authors: Fukuhara K, Ohno A, Ota Y, Senoo Y, Maekawa K, Okuda H, Kurihara M, Okuno A, Niida S, Saito Y, Takikawa O Abstract Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia among elderly patients. A biomarker...	nmrlearner	Journal club	0	03-26-2013 01:30 PM
NMR-based metabolomics of mammalian cell and tissue cultures NMR-based metabolomics of mammalian cell and tissue cultures Abstract NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a quantum mechanical total line shape analysis were utilized to quantify 30 metabolites such as amino acids, Krebs cycle intermediates, activated sugars, cofactors, and others in both media and cell extracts. The impact of bioreactor scale and addition of...	nmrlearner	Journal club	0	03-09-2011 04:19 AM
NMR-based metabolomics of mammalian cell and tissue cultures. NMR-based metabolomics of mammalian cell and tissue cultures. NMR-based metabolomics of mammalian cell and tissue cultures. J Biomol NMR. 2011 Mar 4; Authors: Aranibar N, Borys M, Mackin NA, Ly V, Abu-Absi N, Abu-Absi S, Niemitz M, Schilling B, Li ZJ, Brock B, Russell RJ, Tymiak A, Reily MD NMR spectroscopy was used to evaluate growth media and the cellular metabolome in two systems of interest to biomedical research. The first of these was a Chinese hamster ovary cell line engineered to express a recombinant protein. Here, NMR spectroscopy and a...	nmrlearner	Journal club	0	03-05-2011 01:02 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off