[NMR paper] NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel
We describe approaches for the preparation of membrane proteins in detergent micelles and lipid bilayers for solution and magic angle spinning NMR studies, respectively, as exemplified by the human voltage dependent anion channel 1 (hVDAC1). Here, we report protocols for the preparation of homogenous samples of recombinant hVDAC1 in detergent micelles and lipid two-dimensional crystals yielding high resolution NMR spectra. Procedures are described for the recombinant production of stable-isotope...
[NMR paper] NMR studies of lipid regulation of the K+ channel KcsA.
NMR studies of lipid regulation of the K+ channel KcsA.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif NMR studies of lipid regulation of the K+ channel KcsA.
Biochim Biophys Acta Biomembr. 2020 Oct 13;:183491
Authors: Zhang D, Howarth GS, Parkin LA, McDermott AE
Abstract
The membrane environment, including specific lipid characteristics, plays important roles in the folding, stability, and gating of the prokaryotic potassium channel KcsA. Here we...
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[NMR paper] Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Strategies for Efficient Sample Preparation for Dynamic Nuclear Polarization Solid-State NMR of Biological Macromolecules.
Methods Mol Biol. 2018;1688:133-154
Authors: Itin B, Sergeyev IV
Abstract
Solid-state NMR (SSNMR) is a powerful tool for the elucidation...
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[NMR paper] Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.
Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.
Related Articles Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR.
Methods Mol Biol. 2017;1635:345-358
Authors: Lacabanne D, Kunert B, Gardiennet C, Meier BH, Bo Ckmann A
Abstract
Conformational studies of membrane proteins remain a challenge in the field of structural biology, and in particular the investigation of the proteins in a native-like lipid environment. Solid-state NMR presents a valuable opportunity for...
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[NMR paper] Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Related Articles Probing the Interaction of the Potassium Channel Modulating KCNE1 in Lipid Bilayers via Solid-State NMR Spectroscopy.
Magn Reson Chem. 2017 Feb 23;:
Authors: Zhang R, Sahu ID, Comer RG, Maltsev S, Dabney-Smith C, Lorigan GA
Abstract
KCNE1 is known to modulate the voltage-gated potassium channel ? subunit KCNQ1 to generate slowly activating potassium currents. This potassium channel is essential...
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[NMR paper] Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Related Articles Fluorine-19 NMR and computational quantification of isoflurane binding to the voltage-gated sodium channel NaChBac.
Proc Natl Acad Sci U S A. 2016 Nov 15;:
Authors: Kinde MN, Bondarenko V, Granata D, Bu W, Grasty KC, Loll PJ, Carnevale V, Klein ML, Eckenhoff RG, Tang P, Xu Y
Abstract
Voltage-gated sodium channels (NaV) play an important role in general anesthesia. Electrophysiology measurements...
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[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
J Biomol NMR. 2013 Dec 20;
Authors: Lu GJ, Opella SJ
Abstract
Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...
[NMR paper] 1H-NMR spectroscopy as a means of monitoring nephrotoxicity as exemplified by studies
1H-NMR spectroscopy as a means of monitoring nephrotoxicity as exemplified by studies with cephaloridine.
Related Articles 1H-NMR spectroscopy as a means of monitoring nephrotoxicity as exemplified by studies with cephaloridine.
Hum Exp Toxicol. 1992 Jan;11(1):35-41
Authors: Murgatroyd LB, Pickford RJ, Smith IK, Wilson ID, Middleton BJ
1. Male albino rats were dosed intravenously with either 0.9% saline or cephaloridine in saline at doses of 650, 750 or 950 mg kg-1 d-1 for 7 d. 2. Urine analysis on day 3, after two doses of cephaloridine...
NMR Spectroscopic Studies of Ion Channels in Lipid Bilayers: Sample Preparation Strategies Exemplified by the Voltage Dependent Anion Channel
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