Related ArticlesAn NMR spectroscopic identity test for the control of the capsular polysaccharide from Haemophilus influenzae type b.
Biologicals. 2000 Sep;28(3):175-83
Authors: Lemercinier X, Jones C
We describe the use of Nuclear Magnetic Resonance (NMR) spectroscopy to control the identity of purified bulk capsular polysaccharide [called poly(ribosylribitolphosphate) or PRP] from Haemophilus influenzae type b (Hib), and derivatised forms, used in the production of Hib polysaccharide-protein conjugate vaccines. We describe the approaches we have developed to validate this test.
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10-13-2011 01:26 AM
The use of 1Hâ??31P GHMBC and covariance NMR to unambiguously determine phosphate ester linkages in complex polysaccharide mixtures
The use of 1Hâ??31P GHMBC and covariance NMR to unambiguously determine phosphate ester linkages in complex polysaccharide mixtures
Abstract Poly- and oligo-saccharides are commonly employed as antigens in many vaccines. These antigens contain phosphoester structural elements that are crucial to the antigenicity, and hence the effectiveness of the vaccine. Nuclear Magnetic Resonance (NMR) is a powerful tool for the site-specific identification of phosphoesters in saccharides. We describe here two advances in the characterization of phosphoesters in saccharides: (1) the use of 1Hâ??31P...
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09-19-2011 04:45 AM
[NMR paper] Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharide
Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production.
Related Articles Full 1H NMR assignment and detailed O-acetylation patterns of capsular polysaccharides from Neisseria meningitidis used in vaccine production.
Carbohydr Res. 1996 Dec 24;296:83-96
Authors: Lemercinier X, Jones C
We report essentially complete 1H NMR assignments for the capsular polysaccharides from Neisseria meningitidis serotypes A, C, W-135, and Y. These polysaccharides are components...
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