NMR paper The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplote

		BioNMR > Educational resources > Journal club
[NMR paper] The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplote

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-22-2010, 02:20 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplote

The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi.

Related Articles The NMR solution structure of the pheromone Er-11 from the ciliated protozoan Euplotes raikovi.

Protein Sci. 1996 Aug;5(8):1512-22

Authors: LuginbÃ¼hl P, Wu J, Zerbe O, Ortenzi C, Luporini P, WÃ¼thrich K

The NMR solution structure of the pheromone Er-11, a 39-residue protein from the ciliated protozoan Euplotes raikovi, was calculated with the distance geometry program DIANA from 449 NOE upper distance constraints and 97 dihedral angle constraints, and the program OPAL was employed for structure refinement by molecular mechanics energy minimization in a water bath. For a group of 20 conformers used to characterize the solution structure, the average of the pairwise RMS deviations from the mean structure calculated for the backbone heavy atoms N, C alpha, and C' of residues 2-38 was 0.30 A. The molecular architecture is dominated by an up-down-up bundle of three short helices with residues 2-9, 12-19, and 22-32, which is closely similar to the previously determined structures of the homologous pheromones Er-1, Er-2, and Er-10. This finding provides structural evidence for the capability shown by these pheromones to compete with each other in binding reactions to their cell-surface receptors.

PMID: 8844842 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] NMR solution structure of attractin, a water-borne protein pheromone from the mollusk NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. Related Articles NMR solution structure of attractin, a water-borne protein pheromone from the mollusk Aplysia californica. Biochemistry. 2003 Aug 26;42(33):9970-9 Authors: Garimella R, Xu Y, Schein CH, Rajarathnam K, Nagle GT, Painter SD, Braun W Water-borne protein pheromones are essential for coordination of reproductive activities in many marine organisms. In this paper, we describe the first structure of a pheromone protein from a...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiologica NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. Related Articles NMR structure of the unliganded Bombyx mori pheromone-binding protein at physiological pH. FEBS Lett. 2002 Nov 6;531(2):314-8 Authors: Lee D, Damberger FF, Peng G, Horst R, Güntert P, Nikonova L, Leal WS, Wüthrich K The nuclear magnetic resonance structure of the unliganded pheromone-binding protein (PBP) from Bombyx mori at pH above 6.5, BmPBP(B), consists of seven helices with residues 3-8, 16-22, 29-32, 46-59, 70-79, 84-100, and...	nmrlearner	Journal club	0	11-24-2010 08:58 PM
[NMR paper] NMR structure reveals intramolecular regulation mechanism for pheromone binding and r NMR structure reveals intramolecular regulation mechanism for pheromone binding and release. Related Articles NMR structure reveals intramolecular regulation mechanism for pheromone binding and release. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14374-9 Authors: Horst R, Damberger F, Luginbühl P, Güntert P, Peng G, Nikonova L, Leal WS, Wüthrich K Odorants are transmitted by small hydrophobic molecules that cross the aqueous sensillar lymph surrounding the dendrites of the olfactory neurons to stimulate the olfactory receptors. In insects, the...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds. Related Articles NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds. J Mol Biol. 2001 Nov 9;313(5):923-31 Authors: Zahn R, Damberger F, Ortenzi C, Luporini P, Wüthrich K The NMR solution structure of the 51 residue pheromone Er-23 from the ciliated protozoan Euplotes raikovi (Er) was calculated with the torsion angle dynamics program DYANA from 582 nuclear Overhauser enhancement (NOE) upper...	nmrlearner	Journal club	0	11-19-2010 08:44 PM
[NMR paper] NMR assignment of the A form of the pheromone-binding protein of Bombyx mori. NMR assignment of the A form of the pheromone-binding protein of Bombyx mori. Related Articles NMR assignment of the A form of the pheromone-binding protein of Bombyx mori. J Biomol NMR. 2001 Jan;19(1):79-80 Authors: Horst R, Damberger F, Peng G, Nikonova L, Leal WS, Wüthrich K	nmrlearner	Journal club	0	11-19-2010 08:32 PM
NMR structure note: solution structure of the core domain of MESD that is essential f NMR structure note: solution structure of the core domain of MESD that is essential for proper folding of LRP5/6. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR structure note: solution structure of the core domain of MESD that is essential for proper folding of LRP5/6. J Biomol NMR. 2010 Aug;47(4):283-8 Authors: Chen J, Li Q, Liu CC, Zhou B, Bu G, Wang J	nmrlearner	Journal club	0	09-24-2010 03:50 AM
[NMR paper] The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes raikovi. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles The NMR solution structure of the pheromone Er-1 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1994 Sep;3(9):1527-36 Authors: Mronga S, LuginbÃ¼hl P, Brown LR, Ortenzi C,...	nmrlearner	Journal club	0	08-22-2010 03:29 AM
[NMR paper] The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif The NMR solution structure of the pheromone Er-2 from the ciliated protozoan Euplotes raikovi. Protein Sci. 1994 Sep;3(9):1515-26 Authors: Ottiger M, Szyperski T, LuginbÃ¼hl P, Ortenzi C, Luporini P,...	nmrlearner	Journal club	0	08-22-2010 03:29 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off