NMR paper NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain:

		BioNMR > Educational resources > Journal club
[NMR paper] NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain:

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-17-2010, 11:06 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain:

NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction.

Related Articles NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction.

Biochemistry. 1998 Jun 2;37(22):7929-40

Authors: Wang H, Kurochkin AV, Pang Y, Hu W, Flynn GC, Zuiderweg ER

The solution structure of the 21 kDa substrate-binding domain of the Escherichia coli Hsp70-chaperone protein DnaK (DnaK 386-561) has been determined to a precision of 1.00 A (backbone of the beta-domain) from 1075 experimental restraints obtained from multinuclear, multidimensional NMR experiments. The domain is observed to bind to its own C-terminus and offers a preview of the interaction of this chaperone with other proteins. The bound protein region is tightly held at a single amino acid position (a leucyl residue) that is buried in a deep pocket lined with conserved hydrophobic residues. A second hydrophobic binding site was identified using paramagnetically labeled peptides. It is located in a region close to the N-terminus of the domain and may constitute the allosteric region that links substrate-binding affinity with nucleotide binding in the Hsp70 chaperones.

PMID: 9609686 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes. Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes. Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: pertinence for determining magnetic axes in paramagnetic substrate complexes. J Inorg Biochem. 2010 Oct;104(10):1063-70 Authors: Du Z, Unno M, Matsui T, Ikeda-Saito M, La Mar GN Proton 2D NMR was used to confirm in solution a highly conserved portion of the molecular structure upon substrate loss for the...	nmrlearner	Journal club	0	02-10-2011 03:51 PM
[NMR paper] Folding and domain-domain interactions of the chaperone PapD measured by 19F NMR. Folding and domain-domain interactions of the chaperone PapD measured by 19F NMR. Related Articles Folding and domain-domain interactions of the chaperone PapD measured by 19F NMR. Biochemistry. 2004 Nov 2;43(43):13775-86 Authors: Bann JG, Frieden C The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the...	nmrlearner	Journal club	0	11-24-2010 10:03 PM
[NMR paper] NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells. Related Articles NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells. Biochemistry. 2004 Feb 24;43(7):1854-61 Authors: Rosal R, Pincus MR, Brandt-Rauf PW, Fine RL, Michl J, Wang H We have recently found that a peptide from the mdm-2 binding domain of the p53 protein induced rapid membranolytic necrosis of a variety of different human...	nmrlearner	Journal club	0	11-24-2010 09:25 PM
[NMR paper] Solvent interaction of a Hsp70 chaperone substrate-binding domain investigated with w Solvent interaction of a Hsp70 chaperone substrate-binding domain investigated with water-NOE NMR experiments. Related Articles Solvent interaction of a Hsp70 chaperone substrate-binding domain investigated with water-NOE NMR experiments. Biochemistry. 2003 Sep 30;42(38):11100-8 Authors: Cai S, Stevens SY, Budor AP, Zuiderweg ER The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic...	nmrlearner	Journal club	0	11-24-2010 09:16 PM
[NMR paper] NMR solution structure of the receptor binding domain of human alpha(2)-macroglobulin NMR solution structure of the receptor binding domain of human alpha(2)-macroglobulin. Related Articles NMR solution structure of the receptor binding domain of human alpha(2)-macroglobulin. J Biol Chem. 2000 Jan 14;275(2):1089-94 Authors: Huang W, Dolmer K, Liao X, Gettins PG Human alpha(2)-macroglobulin-proteinase complexes bind to their receptor, the low density lipoprotein receptor-related protein (LRP), through a discrete 138-residue C-terminal receptor binding domain (RBD), which also binds to the beta-amyloid peptide. We have used NMR...	nmrlearner	Journal club	0	11-18-2010 09:15 PM
[NMR paper] NMR solution structure of the periplasmic chaperone FimC. NMR solution structure of the periplasmic chaperone FimC. Related Articles NMR solution structure of the periplasmic chaperone FimC. Nat Struct Biol. 1998 Oct;5(10):885-90 Authors: Pellecchia M, Güntert P, Glockshuber R, Wüthrich K The NMR structure of the 205-residue periplasmic chaperone FimC is presented. This protein consists of two globular domains with immunoglobulin-like folds connected by a 15-residue linker peptide. The relative orientation of the two domains is defined by hydrophobic contacts and an interdomain salt bridge. FimC...	nmrlearner	Journal club	0	11-17-2010 11:15 PM
[NMR paper] NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reve NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR solution structure of a dsRNA binding domain from Drosophila staufen protein reveals homology to the N-terminal domain of ribosomal protein S5. EMBO J. 1995 Jul 17;14(14):3563-71 Authors: Bycroft M, GrÃ¼nert S, Murzin AG, Proctor M, St Johnston D The...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spec Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spectroscopy. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Solution structure of the LexA repressor DNA binding domain determined by 1H NMR spectroscopy. EMBO J. 1994 Sep 1;13(17):3936-44 Authors: Fogh RH, Ottleben G, RÃ¼terjans H, Schnarr M, Boelens R, Kaptein R The structure of the 84 residue DNA binding domain of the Escherichia coli LexA repressor has been determined from...	nmrlearner	Journal club	0	08-22-2010 03:29 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off