NMR paper NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expres

		BioNMR > Educational resources > Journal club
[NMR paper] NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expres

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-21-2010, 11:53 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expres

NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expressed in humoral hypercalcemia of malignancy.

Related Articles NMR solution structure of the [Ala26]parathyroid-hormone-related protein(1-34) expressed in humoral hypercalcemia of malignancy.

Eur J Biochem. 1993 Jan 15;211(1-2):205-11

Authors: Ray FR, Barden JA, Kemp BE

The structure of the biologically active N-terminal domain of human parathyroid-hormone-related protein (residues 1-34) containing an Ala substituted for a His in position 26 was studied by two-dimensional proton NMR spectroscopy. Unambiguous NMR assignments of all backbone and side-chain hydrogens were made with the aid of totally correlated spectroscopy experiments, which provided through-bond 1H-1H connectivities, and NOE spectroscopy, which provided through-space and sequential backbone connectivities. The NMR data were utilized in distance-geometry algorithms to generate a family of structures. The major structural features include two segments of alpha-helix extending from Glu4 to Lys13 and from Leu27 to Thr33, with two turns from Gln16 to Arg19 and Phe22 to His25. A salt-bridge appears likely between Arg20 and Glu30 which may be critical for holding the receptor-binding domain together.

PMID: 8425530 [PubMed - indexed for MEDLINE]

Source: PubMed

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational analysis of antagonists by CD, NMR, and distance geometry calculations. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational analysis of antagonists by CD, NMR, and distance geometry calculations. Biochemistry. 1997 Mar 18;36(11):3300-7 Authors: Maretto S, Mammi S, Bissacco E, Peggion E, Bisello A, Rosenblatt M, Chorev M, Mierke DF ...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] NMR solution structure of the pathogenesis-related protein P14a. NMR solution structure of the pathogenesis-related protein P14a. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR solution structure of the pathogenesis-related protein P14a. J Mol Biol. 1997 Feb 28;266(3):576-93 Authors: Fernández C, Szyperski T, Bruyère T, Ramage P, Mösinger E, Wüthrich K The nuclear magnetic resonance (NMR) structure of the 15 kDa pathogenesis-related protein P14a, which displays antifungicidal activity and is induced in tomato leaves as a...	nmrlearner	Journal club	0	08-22-2010 03:31 PM
[NMR paper] Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational analysis of antagonists by CD, NMR, and distance geometry calculations. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Mono- and bicyclic analogs of parathyroid hormone-related protein. 2. Conformational analysis of antagonists by CD, NMR, and distance geometry calculations. Biochemistry. 1997 Mar 18;36(11):3300-7 Authors: Maretto S, Mammi S, Bissacco E, Peggion E, Bisello A, Rosenblatt M, Chorev M, Mierke DF ...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] NMR solution structure of the pathogenesis-related protein P14a. NMR solution structure of the pathogenesis-related protein P14a. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR solution structure of the pathogenesis-related protein P14a. J Mol Biol. 1997 Feb 28;266(3):576-93 Authors: Fernández C, Szyperski T, Bruyère T, Ramage P, Mösinger E, Wüthrich K The nuclear magnetic resonance (NMR) structure of the 15 kDa pathogenesis-related protein P14a, which displays antifungicidal activity and is induced in tomato leaves as a...	nmrlearner	Journal club	0	08-22-2010 03:03 PM
[NMR paper] Structure of recombinant human parathyroid hormone in solution using multidimensional Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy. Related Articles Structure of recombinant human parathyroid hormone in solution using multidimensional NMR spectroscopy. Biol Chem Hoppe Seyler. 1996 Mar;377(3):175-86 Authors: Gronwald W, Schomburg D, Harder MP, Mayer H, Paulsen J, Wingender E, Wray V The solution structure of human parathyroid hormone, in the form of recombinant prolyl-hPTH(1-84), has been investigated by multidimensional NMR spectroscopy under conditions (aqueous...	nmrlearner	Journal club	0	08-22-2010 02:27 PM
[NMR paper] Solution structure of horse heart ferricytochrome c and detection of redox-related st Solution structure of horse heart ferricytochrome c and detection of redox-related structural changes by high-resolution 1H NMR. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Solution structure of horse heart ferricytochrome c and detection of redox-related structural changes by high-resolution 1H NMR. Biochemistry. 1996 Sep 24;35(38):12275-86 Authors: Qi PX, Beckman RA, Wand AJ A model for the solution structure of horse heart ferricytochrome c has been determined by nuclear magnetic...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
[NMR paper] Conformation of parathyroid hormone antagonists by CD, NMR, and molecular dynamics si Conformation of parathyroid hormone antagonists by CD, NMR, and molecular dynamics simulations. Related Articles Conformation of parathyroid hormone antagonists by CD, NMR, and molecular dynamics simulations. Biopolymers. 1995 Oct;36(4):485-95 Authors: Chorev M, Behar V, Yang Q, Rosenblatt M, Mammi S, Maretto S, Pellegrini M, Peggion E The conformation of two highly potent parathyroid hormone (PTH) antagonists was investigated in water/2,2,2-trifluoroethanol mixtures. The two peptides are derived from the sequence (7-34) of PTH and of...	nmrlearner	Journal club	0	08-22-2010 03:50 AM
[NMR paper] Stabilized NMR structure of human parathyroid hormone(1-34). Stabilized NMR structure of human parathyroid hormone(1-34). http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_FREE_120x27.gif Related Articles Stabilized NMR structure of human parathyroid hormone(1-34). Eur J Biochem. 1993 Jul 15;215(2):315-21 Authors: Barden JA, Cuthbertson RM The structure of the biologically-active N-terminal region of human parathyroid hormone, PTH(1-34), was investigated in the presence of 10% trifluoroethanol using two-dimensional proton...	nmrlearner	Journal club	0	08-22-2010 03:01 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off